The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia
Stroke mostly including ischemic stroke is the second leading mortality and disability worldwide. Oxidative stress injury occurred during ischemic stroke treatment generally. A high amount of reactive oxygen species (ROS) is involved in oxidative stress induction. Transient receptor potential vanill...
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Wiley
2021-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2021/7955791 |
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| author | Tingting Huang Yao Lin Qiongyi Pang Weimin Shen Xiang Chen Fengxia Tu |
| author_facet | Tingting Huang Yao Lin Qiongyi Pang Weimin Shen Xiang Chen Fengxia Tu |
| author_sort | Tingting Huang |
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| description | Stroke mostly including ischemic stroke is the second leading mortality and disability worldwide. Oxidative stress injury occurred during ischemic stroke treatment generally. A high amount of reactive oxygen species (ROS) is involved in oxidative stress induction. Transient receptor potential vanilloid 1 (TRPV1) has been shown to regulate oxidative stress and apoptosis in microglia; however, the detailed mechanisms remain unclear. We aimed to explore whether autophagy-regulated oxidative stress and apoptosis are associated with TRPV1. The model of oxygen and glucose deprivation (OGD/R) in microglia was established. The siRNA of Atg5 and inhibitors and agonists of both autophagy and TRPV1 were involved in our study. Autophagy-related markers Atg5, LC3II/LC3I, and Beclin-1 were measured, and the autophagosome was observed under a transmission electron microscope (TEM). Caspase 3 was detected using ELISA. ROS and JC-1 were detected using flow cytometry. Apoptosis was observed by TUNEL. The results indicated that oxidative stress-induced injury and apoptosis may be impeded by the increasing autophagy, and TRPV1 inhibition could suppress the OGD/R-induced autophagy of microglia. However, the effect of TRPV1’s inhibitor on oxidative stress and apoptosis was not obvious when the autophagy was blocked. These findings suggested that TRPV1 may exhibit antioxidative and antiapoptosis effect on OGD/R-induced microglia. However, the experimental results do not fully demonstrate that the TRPV1-mediated antioxidative and antiapoptosis effect is through the affecting autophagy entirely. |
| format | Article |
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| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Analytical Cellular Pathology |
| spelling | doaj-art-c3d37ceaabd44967846b29995982bb1a2025-02-03T01:08:52ZengWileyAnalytical Cellular Pathology2210-71772210-71852021-01-01202110.1155/2021/79557917955791The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in MicrogliaTingting Huang0Yao Lin1Qiongyi Pang2Weimin Shen3Xiang Chen4Fengxia Tu5Yuying Children’s Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Wenzhou, Zhejiang, ChinaYuying Children’s Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Wenzhou, Zhejiang, ChinaYuying Children’s Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Wenzhou, Zhejiang, ChinaYuying Children’s Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Wenzhou, Zhejiang, ChinaYuying Children’s Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Wenzhou, Zhejiang, ChinaYuying Children’s Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Wenzhou, Zhejiang, ChinaStroke mostly including ischemic stroke is the second leading mortality and disability worldwide. Oxidative stress injury occurred during ischemic stroke treatment generally. A high amount of reactive oxygen species (ROS) is involved in oxidative stress induction. Transient receptor potential vanilloid 1 (TRPV1) has been shown to regulate oxidative stress and apoptosis in microglia; however, the detailed mechanisms remain unclear. We aimed to explore whether autophagy-regulated oxidative stress and apoptosis are associated with TRPV1. The model of oxygen and glucose deprivation (OGD/R) in microglia was established. The siRNA of Atg5 and inhibitors and agonists of both autophagy and TRPV1 were involved in our study. Autophagy-related markers Atg5, LC3II/LC3I, and Beclin-1 were measured, and the autophagosome was observed under a transmission electron microscope (TEM). Caspase 3 was detected using ELISA. ROS and JC-1 were detected using flow cytometry. Apoptosis was observed by TUNEL. The results indicated that oxidative stress-induced injury and apoptosis may be impeded by the increasing autophagy, and TRPV1 inhibition could suppress the OGD/R-induced autophagy of microglia. However, the effect of TRPV1’s inhibitor on oxidative stress and apoptosis was not obvious when the autophagy was blocked. These findings suggested that TRPV1 may exhibit antioxidative and antiapoptosis effect on OGD/R-induced microglia. However, the experimental results do not fully demonstrate that the TRPV1-mediated antioxidative and antiapoptosis effect is through the affecting autophagy entirely.http://dx.doi.org/10.1155/2021/7955791 |
| spellingShingle | Tingting Huang Yao Lin Qiongyi Pang Weimin Shen Xiang Chen Fengxia Tu The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia Analytical Cellular Pathology |
| title | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia |
| title_full | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia |
| title_fullStr | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia |
| title_full_unstemmed | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia |
| title_short | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia |
| title_sort | synergistic effect of trpv1 on oxidative stress induced autophagy and apoptosis in microglia |
| url | http://dx.doi.org/10.1155/2021/7955791 |
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