Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares
The quarter horse is a breed that stands out for its strength, explosiveness, versatility, and use in several equestrian sports. For equines, assisted reproduction presents challenges for artificial insemination and/or in vitro fertilization. This study aimed to characterize a portion of the eFSHR g...
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| Format: | Article |
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University of Mosul, College of Veterinary Medicine
2025-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_187259_729810f70864d49830699f3b43672b49.pdf |
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| author | Roberta A. SIlva Moises L. Moreira Elem }ز Barra Priscilla C. Ramos Lawrence O. Barros Gustavo F. Carneiro Elizabeth M. Barbosa Sebastião T. Rolim Filho Ednaldo S. Filho |
| author_facet | Roberta A. SIlva Moises L. Moreira Elem }ز Barra Priscilla C. Ramos Lawrence O. Barros Gustavo F. Carneiro Elizabeth M. Barbosa Sebastião T. Rolim Filho Ednaldo S. Filho |
| author_sort | Roberta A. SIlva |
| collection | DOAJ |
| description | The quarter horse is a breed that stands out for its strength, explosiveness, versatility, and use in several equestrian sports. For equines, assisted reproduction presents challenges for artificial insemination and/or in vitro fertilization. This study aimed to characterize a portion of the eFSHR gene promoter in Quarter Horses and associate it with the number of embryos produced in the 2021 season. Peripheral blood samples were collected from 92 inseminated mares for several embryos produced, DNA extraction, followed by conventional PCR, purification, and sequencing using the Sanger method. Genetic population parameters and potential binding factor sites were determined. The association of haplotypes with the number of embryos produced was analyzed using the PROC MIXED of SAS (onDemand) with a significance level of 0.05. Three single nucleotide polymorphisms (SNPs) were identified: -217G>T, -195G>A, and 127A>G. Four haplotypes were determined: G/G/A, G/G/G, G/G/GA, and G/GA/G. The wild-type sequence had 74 binding sites, with Sp1 and TFAP-2alpha binding at position -217 and GR binding at position 195. The mutant sequence had 71 identified sites. The eFSHR gene promoter demonstrated polymorphism in Quarter Horses, but there was no association with the number of produced embryos. However, it could be used for other reproductive traits in mares. |
| format | Article |
| id | doaj-art-c3c894c4792b4a5d8e034c9f23f40c78 |
| institution | OA Journals |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-c3c894c4792b4a5d8e034c9f23f40c782025-08-20T01:47:26ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-04-0139233534010.33899/ijvs.2025.148920.3619187259Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse maresRoberta A. SIlva0Moises L. Moreira1Elem }ز Barra2Priscilla C. Ramos3Lawrence O. Barros4Gustavo F. Carneiro5Elizabeth M. Barbosa6Sebastião T. Rolim Filho7Ednaldo S. Filho8Animal Health and Production Institute, Molecular Biology Laboratory, Federal Rural University of the Amazon, Belem, Brazil.Animal Health and Production Institute, Molecular Biology Laboratory, Federal Rural University of the Amazon, Belem, Brazil.Animal Health and Production Institute, Federal Rural University of the Amazon, Belem, BrazilAnimal Health and Production Institute, Molecular Biology Laboratory, Federal Rural University of the Amazon, Belem, Brazil.Department of Veterinary Medicine. Federal Rural University of Pernambuco, Recife, Brazil.Department of Veterinary Medicine. Federal Rural University of Pernambuco, Recife, Brazil.Department of the Collegiate Degree in Education, Federal University of Amapa, Mazagao, BrazilAnimal Health and Production Institute, Molecular Biology Laboratory, Federal Rural University of the Amazon, Belem, Brazil.Animal Health and Production Institute, Federal Rural University of the Amazon, Belem, BrazilThe quarter horse is a breed that stands out for its strength, explosiveness, versatility, and use in several equestrian sports. For equines, assisted reproduction presents challenges for artificial insemination and/or in vitro fertilization. This study aimed to characterize a portion of the eFSHR gene promoter in Quarter Horses and associate it with the number of embryos produced in the 2021 season. Peripheral blood samples were collected from 92 inseminated mares for several embryos produced, DNA extraction, followed by conventional PCR, purification, and sequencing using the Sanger method. Genetic population parameters and potential binding factor sites were determined. The association of haplotypes with the number of embryos produced was analyzed using the PROC MIXED of SAS (onDemand) with a significance level of 0.05. Three single nucleotide polymorphisms (SNPs) were identified: -217G>T, -195G>A, and 127A>G. Four haplotypes were determined: G/G/A, G/G/G, G/G/GA, and G/GA/G. The wild-type sequence had 74 binding sites, with Sp1 and TFAP-2alpha binding at position -217 and GR binding at position 195. The mutant sequence had 71 identified sites. The eFSHR gene promoter demonstrated polymorphism in Quarter Horses, but there was no association with the number of produced embryos. However, it could be used for other reproductive traits in mares.https://www.vetmedmosul.com/article_187259_729810f70864d49830699f3b43672b49.pdfembryoequineefshr genepolymorphismssnp |
| spellingShingle | Roberta A. SIlva Moises L. Moreira Elem }ز Barra Priscilla C. Ramos Lawrence O. Barros Gustavo F. Carneiro Elizabeth M. Barbosa Sebastião T. Rolim Filho Ednaldo S. Filho Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares Iraqi Journal of Veterinary Sciences embryo equine efshr gene polymorphisms snp |
| title | Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares |
| title_full | Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares |
| title_fullStr | Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares |
| title_full_unstemmed | Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares |
| title_short | Novel polymorphisms in the equine FSH receptor (eFSHR) gene and their association with embryo production following artificial insemination in quarter horse mares |
| title_sort | novel polymorphisms in the equine fsh receptor efshr gene and their association with embryo production following artificial insemination in quarter horse mares |
| topic | embryo equine efshr gene polymorphisms snp |
| url | https://www.vetmedmosul.com/article_187259_729810f70864d49830699f3b43672b49.pdf |
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