Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics
Nowadays, there is an increasing need for sensitive real-time measurements of various analytes and monitoring of industrial products and environmental processes. Herein, we describe a fluorescence spectrometer in continuous flow mode in which the sample is fed to the flow cell using a peristaltic pu...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/2921417 |
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| author | Nader Shokoufi Maryam Vosough Mona Rahimzadegan-Asl Atefeh Abbasi-Ahd Mahsa Khatibeghdami |
| author_facet | Nader Shokoufi Maryam Vosough Mona Rahimzadegan-Asl Atefeh Abbasi-Ahd Mahsa Khatibeghdami |
| author_sort | Nader Shokoufi |
| collection | DOAJ |
| description | Nowadays, there is an increasing need for sensitive real-time measurements of various analytes and monitoring of industrial products and environmental processes. Herein, we describe a fluorescence spectrometer in continuous flow mode in which the sample is fed to the flow cell using a peristaltic pump. The excitation beam is introduced to the sample chamber by an optical fiber. The fluorescence emitted upon excitation is collected at the right angle using another optical fiber and then transmitted to the fluorescence spectrometer which utilizes an array detector. The array detection, as a key factor in process analytical chemistry, made the fluorescence spectrometer suited for multiwavelength detection of the fluorescence spectrum of the analytes. After optimization of the experimental parameters, the system has been successfully employed for sensitive determination of four fluoroquinolone antibiotics such as ciprofloxacin, ofloxacin, levofloxacin, and moxifloxacin. The linear dynamic ranges of four fluoroquinolones were between 0.25 and 20 μg·mL−1, and the detection limit of the method for ciprofloxacin, ofloxacin, levofloxacin, and moxifloxacin were 81, 36, 35, and 93 ng·mL−1, respectively. Finally, the proposed system is carried out for determination of fluoroquinolones in some pharmaceutical formulations. |
| format | Article |
| id | doaj-art-c3c5f5157148443999822d134f5924f5 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-c3c5f5157148443999822d134f5924f52025-02-03T06:46:36ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/29214172921417Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone AntibioticsNader Shokoufi0Maryam Vosough1Mona Rahimzadegan-Asl2Atefeh Abbasi-Ahd3Mahsa Khatibeghdami4Analytical Instrumentation and Spectroscopy Laboratory, Department of Green Technologies, Chemistry & Chemical Engineering Research Center of Iran, Tehran 14968-13151, IranAnalytical Instrumentation and Spectroscopy Laboratory, Department of Green Technologies, Chemistry & Chemical Engineering Research Center of Iran, Tehran 14968-13151, IranAnalytical Instrumentation and Spectroscopy Laboratory, Department of Green Technologies, Chemistry & Chemical Engineering Research Center of Iran, Tehran 14968-13151, IranAnalytical Instrumentation and Spectroscopy Laboratory, Department of Green Technologies, Chemistry & Chemical Engineering Research Center of Iran, Tehran 14968-13151, IranCancer Center, University of Illinois, Chicago, IL 60612, USANowadays, there is an increasing need for sensitive real-time measurements of various analytes and monitoring of industrial products and environmental processes. Herein, we describe a fluorescence spectrometer in continuous flow mode in which the sample is fed to the flow cell using a peristaltic pump. The excitation beam is introduced to the sample chamber by an optical fiber. The fluorescence emitted upon excitation is collected at the right angle using another optical fiber and then transmitted to the fluorescence spectrometer which utilizes an array detector. The array detection, as a key factor in process analytical chemistry, made the fluorescence spectrometer suited for multiwavelength detection of the fluorescence spectrum of the analytes. After optimization of the experimental parameters, the system has been successfully employed for sensitive determination of four fluoroquinolone antibiotics such as ciprofloxacin, ofloxacin, levofloxacin, and moxifloxacin. The linear dynamic ranges of four fluoroquinolones were between 0.25 and 20 μg·mL−1, and the detection limit of the method for ciprofloxacin, ofloxacin, levofloxacin, and moxifloxacin were 81, 36, 35, and 93 ng·mL−1, respectively. Finally, the proposed system is carried out for determination of fluoroquinolones in some pharmaceutical formulations.http://dx.doi.org/10.1155/2020/2921417 |
| spellingShingle | Nader Shokoufi Maryam Vosough Mona Rahimzadegan-Asl Atefeh Abbasi-Ahd Mahsa Khatibeghdami Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics International Journal of Analytical Chemistry |
| title | Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics |
| title_full | Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics |
| title_fullStr | Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics |
| title_full_unstemmed | Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics |
| title_short | Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics |
| title_sort | fiberoptic coupled spectrofluorometer with array detection as a process analytical chemistry tool for continuous flow monitoring of fluoroquinolone antibiotics |
| url | http://dx.doi.org/10.1155/2020/2921417 |
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