Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury
The inactivation and sublethal injury of two strains of Listeria innocua (one collection strain and one wild strain isolated from beetroot juice) suspended in beetroot juice and in model solutions, after high hydrostatic pressure (HHP) were investigated. Changes within the population assessed by p...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2019-01-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.journalssystem.com/pjfns/,100427,0,2.html |
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| author | Justyna Nasiłowska Barbara Sokołowska Monika Fonberg-Broczek |
| author_facet | Justyna Nasiłowska Barbara Sokołowska Monika Fonberg-Broczek |
| author_sort | Justyna Nasiłowska |
| collection | DOAJ |
| description | The inactivation and sublethal injury of two strains of Listeria innocua (one collection strain and one wild strain isolated from beetroot juice) suspended in beetroot juice and in model solutions, after high hydrostatic pressure (HHP) were investigated. Changes within the population assessed by plating count methods of both L. innocua strains suspended in a buffer pH 4.0 were more noticeable than in the natural beetroot juice environment. In beetroot juice the lethal effect was reported after 1 min of pressure treatment at 400 MPa for the collection strain. In the case of the wild type strain, exposure to the maximal parameters of the compression process (400 MPa, 10 min) decreased the population number below 1 log (CFU/mL) but did not cause complete injury. The collection strain of L. innocua was easier to inactivate in beetroot juice than the strain isolated from this environment. The maximum level of sublethal injury was observed when the cells were suspended in a buffer pH 7.0. Structural damage in cell membranes after HHP processing was observed using a transmission electron microscope (TEM). |
| format | Article |
| id | doaj-art-c3986558c4404edeacf99e3eb4fd7101 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-c3986558c4404edeacf99e3eb4fd71012025-02-03T03:14:30ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072019-01-01691455210.31883/pjfns-2019-0004100427Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal InjuryJustyna Nasiłowska0Barbara Sokołowska1Monika Fonberg-Broczek2Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Fruit and Vegetable Product Technology, 36 Rakowiecka str., 02-532 Warsaw, PolandProf. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Fruit and Vegetable Product Technology, 36 Rakowiecka str., 02-532 Warsaw, PolandInstitute of High Pressure Physics, Polish Academy of Sciences, Laboratory of Biological Materials, 29/37 Sokołowska str., 01-142 Warsaw, PolandThe inactivation and sublethal injury of two strains of Listeria innocua (one collection strain and one wild strain isolated from beetroot juice) suspended in beetroot juice and in model solutions, after high hydrostatic pressure (HHP) were investigated. Changes within the population assessed by plating count methods of both L. innocua strains suspended in a buffer pH 4.0 were more noticeable than in the natural beetroot juice environment. In beetroot juice the lethal effect was reported after 1 min of pressure treatment at 400 MPa for the collection strain. In the case of the wild type strain, exposure to the maximal parameters of the compression process (400 MPa, 10 min) decreased the population number below 1 log (CFU/mL) but did not cause complete injury. The collection strain of L. innocua was easier to inactivate in beetroot juice than the strain isolated from this environment. The maximum level of sublethal injury was observed when the cells were suspended in a buffer pH 7.0. Structural damage in cell membranes after HHP processing was observed using a transmission electron microscope (TEM).http://www.journalssystem.com/pjfns/,100427,0,2.htmllisteria innocuabeetroot juicehigh hydrostatic pressuresublethal injurytransmission electron microscopy |
| spellingShingle | Justyna Nasiłowska Barbara Sokołowska Monika Fonberg-Broczek Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury Polish Journal of Food and Nutrition Sciences listeria innocua beetroot juice high hydrostatic pressure sublethal injury transmission electron microscopy |
| title | Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury |
| title_full | Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury |
| title_fullStr | Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury |
| title_full_unstemmed | Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury |
| title_short | Behavior of Listeria innocua Strains Under Pressure Treatment – Inactivation and Sublethal Injury |
| title_sort | behavior of listeria innocua strains under pressure treatment inactivation and sublethal injury |
| topic | listeria innocua beetroot juice high hydrostatic pressure sublethal injury transmission electron microscopy |
| url | http://www.journalssystem.com/pjfns/,100427,0,2.html |
| work_keys_str_mv | AT justynanasiłowska behavioroflisteriainnocuastrainsunderpressuretreatmentinactivationandsublethalinjury AT barbarasokołowska behavioroflisteriainnocuastrainsunderpressuretreatmentinactivationandsublethalinjury AT monikafonbergbroczek behavioroflisteriainnocuastrainsunderpressuretreatmentinactivationandsublethalinjury |