Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol
Clioquinol has been shown to have anticancer activity in several carcinoma cells. In this study, we preliminarily examined the effect of clioquinol in human SMMC-7721 hepatoma and QSG-7701 normal hepatic cells. Our results indicated that clioquinol did not significantly affect survival of QSG-7701 c...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2015-0034 |
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| author | Huang Zhiwei Wang Lianqiu Chen Lifeng Zhang Yifei Shi Ping |
| author_facet | Huang Zhiwei Wang Lianqiu Chen Lifeng Zhang Yifei Shi Ping |
| author_sort | Huang Zhiwei |
| collection | DOAJ |
| description | Clioquinol has been shown to have anticancer activity in several carcinoma cells. In this study, we preliminarily examined the effect of clioquinol in human SMMC-7721 hepatoma and QSG-7701 normal hepatic cells. Our results indicated that clioquinol did not significantly affect survival of QSG-7701 cells, whereas it reduced cell viability in a concentration- and time-dependent manner in SMMC-7721 cells. Clioquinol did not trigger autophagy and apoptosis, while it induced cell cycle arrest in the S-phase in SMMC- 7721 cells. Additionally, down-regulation of cyclin D1, A2, E1, Cdk2 and up-regulation of p21, p27 were detected after the treatment with clioquinol. The results demonstrated for the first time that clioquinol suppressed cell cycle progression in the S-phase in SMMC-7721 cells via the p21, p27-cyclin E,A/Cdk2 pathway. This suggests that clioquinol may have a therapeutic potential as an anticancer drug for certain malignances. |
| format | Article |
| id | doaj-art-c366976cf2e3476db1225bea27e81b41 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-c366976cf2e3476db1225bea27e81b412025-02-02T09:57:48ZengSciendoActa Pharmaceutica1846-95582015-12-0165446347110.1515/acph-2015-0034acph-2015-0034Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinolHuang Zhiwei0Wang Lianqiu1Chen Lifeng2Zhang Yifei3Shi Ping4Key Lab of Eco-Textile (Ministry of Education), College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620, ChinaKey Lab of Eco-Textile (Ministry of Education), College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620, ChinaKey Lab of Eco-Textile (Ministry of Education), College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237, ChinaClioquinol has been shown to have anticancer activity in several carcinoma cells. In this study, we preliminarily examined the effect of clioquinol in human SMMC-7721 hepatoma and QSG-7701 normal hepatic cells. Our results indicated that clioquinol did not significantly affect survival of QSG-7701 cells, whereas it reduced cell viability in a concentration- and time-dependent manner in SMMC-7721 cells. Clioquinol did not trigger autophagy and apoptosis, while it induced cell cycle arrest in the S-phase in SMMC- 7721 cells. Additionally, down-regulation of cyclin D1, A2, E1, Cdk2 and up-regulation of p21, p27 were detected after the treatment with clioquinol. The results demonstrated for the first time that clioquinol suppressed cell cycle progression in the S-phase in SMMC-7721 cells via the p21, p27-cyclin E,A/Cdk2 pathway. This suggests that clioquinol may have a therapeutic potential as an anticancer drug for certain malignances.https://doi.org/10.1515/acph-2015-0034clioquinolhuman smmc-7721 hepatoma cellscell cycle s-phase arrestcyclin e/a-cdk2 |
| spellingShingle | Huang Zhiwei Wang Lianqiu Chen Lifeng Zhang Yifei Shi Ping Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol Acta Pharmaceutica clioquinol human smmc-7721 hepatoma cells cell cycle s-phase arrest cyclin e/a-cdk2 |
| title | Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol |
| title_full | Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol |
| title_fullStr | Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol |
| title_full_unstemmed | Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol |
| title_short | Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol |
| title_sort | induction of cell cycle arrest via the p21 p27 cyclin e a cdk2 pathway in smmc 7721 hepatoma cells by clioquinol |
| topic | clioquinol human smmc-7721 hepatoma cells cell cycle s-phase arrest cyclin e/a-cdk2 |
| url | https://doi.org/10.1515/acph-2015-0034 |
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