Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines
Breast cancer is a common form of cancer that affects women all over the world. Cancer patients have relied on surgery and radiotherapy, chemically derived medications for treatment, which result in several unpleasant side effects. To overcome these issues, medicinal plants would be used as modern p...
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Elsevier
2025-05-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625001997 |
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| author | Abdullah F. Aldairi Sarah A. Alharbi Moodi S. Alharbi Ahmad Alghamdi Anmar Khan Mazen Ghaith Hani Alnadwi Abdullah Alshehri Asma Alsubaihi Ayman Alhazmi |
| author_facet | Abdullah F. Aldairi Sarah A. Alharbi Moodi S. Alharbi Ahmad Alghamdi Anmar Khan Mazen Ghaith Hani Alnadwi Abdullah Alshehri Asma Alsubaihi Ayman Alhazmi |
| author_sort | Abdullah F. Aldairi |
| collection | DOAJ |
| description | Breast cancer is a common form of cancer that affects women all over the world. Cancer patients have relied on surgery and radiotherapy, chemically derived medications for treatment, which result in several unpleasant side effects. To overcome these issues, medicinal plants would be used as modern pharmaceutical agents to treat various diseases, such as cancer. The study aims to evaluate the antiproliferative activities of Commiphora gileadensis (L.) C.Chr extracts against breast cancer cell lines MCF-7 and MDA-MB-231. Methods: Commiphora gileadensis (L.) C.Chr was harvested and cleaned; chemical extraction was applied using acetone and methanol, then cell viability was measured. The active extract was characterised via untargeted lipidomics analysis. Results: The acetone extract of Commiphora gileadensis (L.) C.Chr showed growth inhibition against MCF-7 and MDA-MB-231 cell lines with IC50 (57.32 μg/mL) and (49.94 μg/mL), respectively. The methanolic extract showed antiproliferative activities against the MCF-7 cell line with IC50 (306.7 μg/mL) and the IC50 (273.6 μg/mL) for MDA-MB-231. Untargeted lipidomic analysis revealed the presence of various lipid classes in each extract. In the acetone extract, the most abundant lipid compounds are hexosylceramide (39.1 %) and ceramide (18.6 %). On the other hand, the lipidomics analysis of the methanolic extract showed the composition consists primarily of ceramide (69 %). In conclusion, the acetone and methanol extracts of Commiphora gileadensis (L.) C.Chr exhibited cytotoxic properties against breast cancer cell lines. |
| format | Article |
| id | doaj-art-c3594eef7b8742ffab0a6da785bcb78c |
| institution | OA Journals |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-c3594eef7b8742ffab0a6da785bcb78c2025-08-20T02:09:18ZengElsevierResults in Chemistry2211-71562025-05-011510221610.1016/j.rechem.2025.102216Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell linesAbdullah F. Aldairi0Sarah A. Alharbi1Moodi S. Alharbi2Ahmad Alghamdi3Anmar Khan4Mazen Ghaith5Hani Alnadwi6Abdullah Alshehri7Asma Alsubaihi8Ayman Alhazmi9Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Al Abdeyah, P.O. Box 7607, Makkah 21961, Saudi Arabia; Corresponding author.Laboratory Department, Prince Mohammed Bin Abdulaziz Hospital, Ministry of National Guard-Health Affairs, Al Madinah 41511, Saudi ArabiaLaboratory Department, King Abdulaziz Hospital, Ministry of Health, Makkah, Saudi ArabiaDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Al Abdeyah, P.O. Box 7607, Makkah 21961, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Al Abdeyah, P.O. Box 7607, Makkah 21961, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Al Abdeyah, P.O. Box 7607, Makkah 21961, Saudi ArabiaDepartment of Physiology, Neuroscience Unit, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi ArabiaFaculty of Medicine, Biochemistry Department, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBreast cancer is a common form of cancer that affects women all over the world. Cancer patients have relied on surgery and radiotherapy, chemically derived medications for treatment, which result in several unpleasant side effects. To overcome these issues, medicinal plants would be used as modern pharmaceutical agents to treat various diseases, such as cancer. The study aims to evaluate the antiproliferative activities of Commiphora gileadensis (L.) C.Chr extracts against breast cancer cell lines MCF-7 and MDA-MB-231. Methods: Commiphora gileadensis (L.) C.Chr was harvested and cleaned; chemical extraction was applied using acetone and methanol, then cell viability was measured. The active extract was characterised via untargeted lipidomics analysis. Results: The acetone extract of Commiphora gileadensis (L.) C.Chr showed growth inhibition against MCF-7 and MDA-MB-231 cell lines with IC50 (57.32 μg/mL) and (49.94 μg/mL), respectively. The methanolic extract showed antiproliferative activities against the MCF-7 cell line with IC50 (306.7 μg/mL) and the IC50 (273.6 μg/mL) for MDA-MB-231. Untargeted lipidomic analysis revealed the presence of various lipid classes in each extract. In the acetone extract, the most abundant lipid compounds are hexosylceramide (39.1 %) and ceramide (18.6 %). On the other hand, the lipidomics analysis of the methanolic extract showed the composition consists primarily of ceramide (69 %). In conclusion, the acetone and methanol extracts of Commiphora gileadensis (L.) C.Chr exhibited cytotoxic properties against breast cancer cell lines.http://www.sciencedirect.com/science/article/pii/S2211715625001997Commiphora gileadensis (L.) C.ChrBreast cancerMCF-7MDA-MB-231 |
| spellingShingle | Abdullah F. Aldairi Sarah A. Alharbi Moodi S. Alharbi Ahmad Alghamdi Anmar Khan Mazen Ghaith Hani Alnadwi Abdullah Alshehri Asma Alsubaihi Ayman Alhazmi Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines Results in Chemistry Commiphora gileadensis (L.) C.Chr Breast cancer MCF-7 MDA-MB-231 |
| title | Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines |
| title_full | Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines |
| title_fullStr | Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines |
| title_full_unstemmed | Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines |
| title_short | Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines |
| title_sort | characterisation and purification of commiphora gileadensis extracts with antiproliferative activities on breast cancer cell lines |
| topic | Commiphora gileadensis (L.) C.Chr Breast cancer MCF-7 MDA-MB-231 |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625001997 |
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