Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation
Human gingiva-derived mesenchymal stem cells (GMSCs) are isolated from the gingival propria with promising regenerative, immunomodulatory, and anti-inflammatory properties. Recently, several studies, including ours, have found that GMSCs have the therapeutic potentials of nerve regeneration and skin...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/6544514 |
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| author | Ziyu Ye Yanfang Liang Bihua Lin Yanyun Li Xingxing Chai Jiachun Lian Xueying Zhang Zhengping Che Jincheng Zeng |
| author_facet | Ziyu Ye Yanfang Liang Bihua Lin Yanyun Li Xingxing Chai Jiachun Lian Xueying Zhang Zhengping Che Jincheng Zeng |
| author_sort | Ziyu Ye |
| collection | DOAJ |
| description | Human gingiva-derived mesenchymal stem cells (GMSCs) are isolated from the gingival propria with promising regenerative, immunomodulatory, and anti-inflammatory properties. Recently, several studies, including ours, have found that GMSCs have the therapeutic potentials of nerve regeneration and skin disorders in various types such as the cell itself, cell-free conditioned medium, or extracellular vesicles (EVs). However, the mechanobiological behavior of GMSCs is closely related to the culture conditions. Therefore, the purpose of this study was to evaluate the function of human GMSCs on imiquimod- (IMQ-) induced murine psoriasis-like skin inflammation in two-dimensional (2D) and three-dimensional (3D) culture conditions. Here, we isolated and characterized GMSCs in 2D and 3D culture conditions and found that GMSCs in 2D and 3D infusion can significantly ameliorate the IMQ-induced murine psoriasis-like skin inflammation, reduce the levels of Th1- and Th17-related cytokines IFN-γ, TNF-α, IL-6, IL-17A, IL-17F, IL-21, and IL-22, and upregulate the percentage of spleen CD25+CD3+ T cells while downregulate the percentage of spleen IL-17+CD3+ T cells. In summary, our novel findings reveal that GMSCs in 2D and 3D infusion may possess therapeutic effects in the treatment of psoriasis. |
| format | Article |
| id | doaj-art-c356882b5f0443a19c79e4726ef3d571 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c356882b5f0443a19c79e4726ef3d5712025-02-03T05:51:01ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/6544514Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin InflammationZiyu Ye0Yanfang Liang1Bihua Lin2Yanyun Li3Xingxing Chai4Jiachun Lian5Xueying Zhang6Zhengping Che7Jincheng Zeng8Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDepartment of PathologyDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational ResearchHuman gingiva-derived mesenchymal stem cells (GMSCs) are isolated from the gingival propria with promising regenerative, immunomodulatory, and anti-inflammatory properties. Recently, several studies, including ours, have found that GMSCs have the therapeutic potentials of nerve regeneration and skin disorders in various types such as the cell itself, cell-free conditioned medium, or extracellular vesicles (EVs). However, the mechanobiological behavior of GMSCs is closely related to the culture conditions. Therefore, the purpose of this study was to evaluate the function of human GMSCs on imiquimod- (IMQ-) induced murine psoriasis-like skin inflammation in two-dimensional (2D) and three-dimensional (3D) culture conditions. Here, we isolated and characterized GMSCs in 2D and 3D culture conditions and found that GMSCs in 2D and 3D infusion can significantly ameliorate the IMQ-induced murine psoriasis-like skin inflammation, reduce the levels of Th1- and Th17-related cytokines IFN-γ, TNF-α, IL-6, IL-17A, IL-17F, IL-21, and IL-22, and upregulate the percentage of spleen CD25+CD3+ T cells while downregulate the percentage of spleen IL-17+CD3+ T cells. In summary, our novel findings reveal that GMSCs in 2D and 3D infusion may possess therapeutic effects in the treatment of psoriasis.http://dx.doi.org/10.1155/2022/6544514 |
| spellingShingle | Ziyu Ye Yanfang Liang Bihua Lin Yanyun Li Xingxing Chai Jiachun Lian Xueying Zhang Zhengping Che Jincheng Zeng Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation Stem Cells International |
| title | Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation |
| title_full | Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation |
| title_fullStr | Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation |
| title_full_unstemmed | Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation |
| title_short | Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation |
| title_sort | gingiva derived mesenchymal stem cells attenuate imiquimod imq induced murine psoriasis like skin inflammation |
| url | http://dx.doi.org/10.1155/2022/6544514 |
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