Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae)
Abstract Obligatory parthenogenesis in vertebrates is restricted to squamate reptiles and evolved through hybridisation. Parthenogens can hybridise with sexual species, resulting in individuals with increased ploidy levels. We describe two successive hybridisations of the parthenogenetic butterfly l...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83300-y |
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| author | Eduard Galoyan Roman Nazarov Marie Altmanová Sergey Matveevsky Ivan Kropachev Dmitrij Dedukh Eugene Iryshkov Mark Pankin Natalia Sopilko Oleg Nikolaev Nikolai Orlov Marine Arakelyan Jiří Klíma Evgeniya Solovyeva Tao Nguyen Lukáš Kratochvíl |
| author_facet | Eduard Galoyan Roman Nazarov Marie Altmanová Sergey Matveevsky Ivan Kropachev Dmitrij Dedukh Eugene Iryshkov Mark Pankin Natalia Sopilko Oleg Nikolaev Nikolai Orlov Marine Arakelyan Jiří Klíma Evgeniya Solovyeva Tao Nguyen Lukáš Kratochvíl |
| author_sort | Eduard Galoyan |
| collection | DOAJ |
| description | Abstract Obligatory parthenogenesis in vertebrates is restricted to squamate reptiles and evolved through hybridisation. Parthenogens can hybridise with sexual species, resulting in individuals with increased ploidy levels. We describe two successive hybridisations of the parthenogenetic butterfly lizards (genus Leiolepis) in Vietnam with a parental sexual species. Contrary to previous proposals, we document that parthenogenetic L. guentherpetersi has mitochondrial DNA and two haploid sets from L. guttata and one from L. reevesii, suggesting that it is the result of a backcross of a parthenogenetic L. guttata × L. reevesii hybrid with a L. guttata male increasing ploidy from 2n to 3n. Within the range of L. guentherpetersi, we found an adult tetraploid male with three L. guttata and one L. reevesii haploid genomes. It probably originated from fertilisation of an unreduced triploid L. guentherpetersi egg by a L. guttata sperm. Although its external morphology resembles that of the maternal species, it possessed exceptionally large erythrocytes and was likely sterile. As increased ploidy level above triploidy or tetraploidy appears to be harmful for amniotes, all-female asexual lineages should evolve a strategy to prevent incorporation of other haploid genomes from a sexual species by avoiding fertilisation by sexual males. |
| format | Article |
| id | doaj-art-c3468b48065648789818b4f507b9e09d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-c3468b48065648789818b4f507b9e09d2025-01-26T12:28:03ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-024-83300-yNatural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae)Eduard Galoyan0Roman Nazarov1Marie Altmanová2Sergey Matveevsky3Ivan Kropachev4Dmitrij Dedukh5Eugene Iryshkov6Mark Pankin7Natalia Sopilko8Oleg Nikolaev9Nikolai Orlov10Marine Arakelyan11Jiří Klíma12Evgeniya Solovyeva13Tao Nguyen14Lukáš Kratochvíl15Severtsov Institute of Ecology and Evolution of the Russian Academy of SciencesZoological Museum of Moscow State UniversityLaboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of SciencesVavilov Institute of General Genetics of the Russian Academy of SciencesSevertsov Institute of Ecology and Evolution of the Russian Academy of SciencesLaboratory of Non-Mendelian Evolution, Institute of Animal Physiology and Genetics, Czech Academy of SciencesSevertsov Institute of Ecology and Evolution of the Russian Academy of SciencesSevertsov Institute of Ecology and Evolution of the Russian Academy of SciencesSevertsov Institute of Ecology and Evolution of the Russian Academy of SciencesSevertsov Institute of Ecology and Evolution of the Russian Academy of SciencesZoological Institute, Russian Academy of SciencesDepartment of Zoology, Biological Faculty of Yerevan State UniversityLaboratory of Cell Regeneration and Plasticity, Institute of Animal Physiology and Genetics, Czech Academy of SciencesZoological Museum of Moscow State UniversityVietnam Academy of Science and TechnologyDepartment of Ecology, Faculty of Science, Charles UniversityAbstract Obligatory parthenogenesis in vertebrates is restricted to squamate reptiles and evolved through hybridisation. Parthenogens can hybridise with sexual species, resulting in individuals with increased ploidy levels. We describe two successive hybridisations of the parthenogenetic butterfly lizards (genus Leiolepis) in Vietnam with a parental sexual species. Contrary to previous proposals, we document that parthenogenetic L. guentherpetersi has mitochondrial DNA and two haploid sets from L. guttata and one from L. reevesii, suggesting that it is the result of a backcross of a parthenogenetic L. guttata × L. reevesii hybrid with a L. guttata male increasing ploidy from 2n to 3n. Within the range of L. guentherpetersi, we found an adult tetraploid male with three L. guttata and one L. reevesii haploid genomes. It probably originated from fertilisation of an unreduced triploid L. guentherpetersi egg by a L. guttata sperm. Although its external morphology resembles that of the maternal species, it possessed exceptionally large erythrocytes and was likely sterile. As increased ploidy level above triploidy or tetraploidy appears to be harmful for amniotes, all-female asexual lineages should evolve a strategy to prevent incorporation of other haploid genomes from a sexual species by avoiding fertilisation by sexual males.https://doi.org/10.1038/s41598-024-83300-yHybridisationLeiolepisParthenogenesisTetraploidyMeiosisVietnam |
| spellingShingle | Eduard Galoyan Roman Nazarov Marie Altmanová Sergey Matveevsky Ivan Kropachev Dmitrij Dedukh Eugene Iryshkov Mark Pankin Natalia Sopilko Oleg Nikolaev Nikolai Orlov Marine Arakelyan Jiří Klíma Evgeniya Solovyeva Tao Nguyen Lukáš Kratochvíl Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) Scientific Reports Hybridisation Leiolepis Parthenogenesis Tetraploidy Meiosis Vietnam |
| title | Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) |
| title_full | Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) |
| title_fullStr | Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) |
| title_full_unstemmed | Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) |
| title_short | Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) |
| title_sort | natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards leiolepis agamidae |
| topic | Hybridisation Leiolepis Parthenogenesis Tetraploidy Meiosis Vietnam |
| url | https://doi.org/10.1038/s41598-024-83300-y |
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