Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes
Plant growth-promoting bacteria (PGPB) are free-living microorganisms that actively reside in the rhizosphere and affect plant growth and development. These bacteria employ their own metabolic system to fix nitrogen, solubilize phosphate, and secrete hormones to directly impact the metabolism of pla...
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2025-04-01
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| author | Priya Katiyar Neha Pandey Boby Varghese Keshav Kant Sahu |
| author_facet | Priya Katiyar Neha Pandey Boby Varghese Keshav Kant Sahu |
| author_sort | Priya Katiyar |
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| description | Plant growth-promoting bacteria (PGPB) are free-living microorganisms that actively reside in the rhizosphere and affect plant growth and development. These bacteria employ their own metabolic system to fix nitrogen, solubilize phosphate, and secrete hormones to directly impact the metabolism of plants. Generating sustainable agricultural production under various environmental stresses requires a detailed understanding of mechanisms that bacteria use to promote plant growth. In the present study, <i>Pseudomonas aeruginosa</i> (MW843625), a PGP soil bacterium with a minimum inhibitory concentration (MIC) of 150 mM against fluoride (F), was isolated from agricultural fields of Chhattisgarh, India, and was assessed for remedial and PGP potential. This study concentrated on biomass accumulation, nutrient absorption, and oxidative stress tolerance in plants involving antioxidative enzymes. By determining MDA accumulation and ROS (O<sub>2</sub>•<sup>−</sup> and H<sub>2</sub>O<sub>2</sub>) in <i>Oryza sativa</i> L. under F (50 ppm) stress, oxidative stress tolerance was assessed. The results showed that inoculation with <i>P. aeruginosa</i> enhanced the ability of <i>O. sativa</i> L. seedlings to absorb nutrients and increased the amounts of total chlorophyll (Chl), total soluble protein, and biomass. In contrast to plants cultivated under F-stress alone, those inoculated with <i>P. aeruginosa</i> along with F showed considerably reduced concentrations of F in their roots, shoots, and grains. The alleviation of deleterious effects of F-stress on plants owing to <i>P. aeruginosa</i> inoculation has been associated with improved activity/upregulation of antioxidative genes (SOD, CAT, and APX) in comparison to only F-subjected plants, which resulted in lower O<sub>2</sub>•<sup>−</sup>, H<sub>2</sub>O<sub>2</sub>, and MDA content. Additionally, it has also been reflected from our study that <i>P. aeruginosa</i> has the potential to increase the activities of soil enzymes such as urease, phosphatase, dehydrogenase, nitrate reductase, and cellulase. Accordingly, the findings of the conducted study suggest that <i>P. aeruginosa</i> can be exploited not only as an ideal candidate for bioremediation but also for enhancing soil fertility and the promotion of growth and development of <i>O. sativa</i> L. under F contamination. |
| format | Article |
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| spelling | doaj-art-c33dbadaf3274db38ee8df8e825c5eb22025-08-20T02:28:19ZengMDPI AGPlants2223-77472025-04-01148122310.3390/plants14081223Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric EnzymesPriya Katiyar0Neha Pandey1Boby Varghese2Keshav Kant Sahu3School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, IndiaSchool of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, IndiaCentre for Academic Success in Science and Engineering, University of KwaZulu-Natal, Durban 4001, South AfricaSchool of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, IndiaPlant growth-promoting bacteria (PGPB) are free-living microorganisms that actively reside in the rhizosphere and affect plant growth and development. These bacteria employ their own metabolic system to fix nitrogen, solubilize phosphate, and secrete hormones to directly impact the metabolism of plants. Generating sustainable agricultural production under various environmental stresses requires a detailed understanding of mechanisms that bacteria use to promote plant growth. In the present study, <i>Pseudomonas aeruginosa</i> (MW843625), a PGP soil bacterium with a minimum inhibitory concentration (MIC) of 150 mM against fluoride (F), was isolated from agricultural fields of Chhattisgarh, India, and was assessed for remedial and PGP potential. This study concentrated on biomass accumulation, nutrient absorption, and oxidative stress tolerance in plants involving antioxidative enzymes. By determining MDA accumulation and ROS (O<sub>2</sub>•<sup>−</sup> and H<sub>2</sub>O<sub>2</sub>) in <i>Oryza sativa</i> L. under F (50 ppm) stress, oxidative stress tolerance was assessed. The results showed that inoculation with <i>P. aeruginosa</i> enhanced the ability of <i>O. sativa</i> L. seedlings to absorb nutrients and increased the amounts of total chlorophyll (Chl), total soluble protein, and biomass. In contrast to plants cultivated under F-stress alone, those inoculated with <i>P. aeruginosa</i> along with F showed considerably reduced concentrations of F in their roots, shoots, and grains. The alleviation of deleterious effects of F-stress on plants owing to <i>P. aeruginosa</i> inoculation has been associated with improved activity/upregulation of antioxidative genes (SOD, CAT, and APX) in comparison to only F-subjected plants, which resulted in lower O<sub>2</sub>•<sup>−</sup>, H<sub>2</sub>O<sub>2</sub>, and MDA content. Additionally, it has also been reflected from our study that <i>P. aeruginosa</i> has the potential to increase the activities of soil enzymes such as urease, phosphatase, dehydrogenase, nitrate reductase, and cellulase. Accordingly, the findings of the conducted study suggest that <i>P. aeruginosa</i> can be exploited not only as an ideal candidate for bioremediation but also for enhancing soil fertility and the promotion of growth and development of <i>O. sativa</i> L. under F contamination.https://www.mdpi.com/2223-7747/14/8/1223antioxidative defensefluorideplant growth-promoting bacteria (PGPB)<i>O. sativa</i> L.<i>Pseudomonas aeruginosa</i>reactive oxygen species (ROS) |
| spellingShingle | Priya Katiyar Neha Pandey Boby Varghese Keshav Kant Sahu Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes Plants antioxidative defense fluoride plant growth-promoting bacteria (PGPB) <i>O. sativa</i> L. <i>Pseudomonas aeruginosa</i> reactive oxygen species (ROS) |
| title | Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes |
| title_full | Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes |
| title_fullStr | Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes |
| title_full_unstemmed | Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes |
| title_short | Biopriming of <i>Pseudomonas aeruginosa</i> Abates Fluoride Toxicity in <i>Oryza sativa</i> L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes |
| title_sort | biopriming of i pseudomonas aeruginosa i abates fluoride toxicity in i oryza sativa i l by restricting fluoride accumulation enhancing antioxidative system and boosting activities of rhizospheric enzymes |
| topic | antioxidative defense fluoride plant growth-promoting bacteria (PGPB) <i>O. sativa</i> L. <i>Pseudomonas aeruginosa</i> reactive oxygen species (ROS) |
| url | https://www.mdpi.com/2223-7747/14/8/1223 |
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