Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow
Genome RNA was extracted from bone marrow of pig (Duroc × Landrace × Yorkshire) and PR-39 mRNA amplified through PT-PCR with special primers. A DNA fragment about 349 bp in length was obtained. The PCR product was cloned into pGEM-T vector and sequedced. Sequence analysis suggested that this fragmen...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2002-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0685 |
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| Summary: | Genome RNA was extracted from bone marrow of pig (Duroc × Landrace × Yorkshire) and PR-39 mRNA amplified through PT-PCR with special primers. A DNA fragment about 349 bp in length was obtained. The PCR product was cloned into pGEM-T vector and sequedced. Sequence analysis suggested that this fragment was partial sequence of pr-39 gene cDNA and coded 113 amino acid residues, which constituted the major part of pr-39 mature peptide. The gene fragment gained in this study was consistent with the porcine pr-39 cDNA partial sequence in Genebank. |
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| ISSN: | 1008-9209 2097-5155 |