Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis
Background. Oral squamous cell carcinoma (OSCC) is the most common oral malignancy that preferentially spreads to the cervical lymph node which, when involved, complicates the anticancer therapy and threatens the patient life. It was suggested that lymph node metastasis may be facilitated by lymphan...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2017/7285656 |
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| author | Manar A. Abdul-Aziz Amina K. Amin Dalia H. El-Rouby Olfat G. Shaker |
| author_facet | Manar A. Abdul-Aziz Amina K. Amin Dalia H. El-Rouby Olfat G. Shaker |
| author_sort | Manar A. Abdul-Aziz |
| collection | DOAJ |
| description | Background. Oral squamous cell carcinoma (OSCC) is the most common oral malignancy that preferentially spreads to the cervical lymph node which, when involved, complicates the anticancer therapy and threatens the patient life. It was suggested that lymph node metastasis may be facilitated by lymphangiogenesis. VEGF-C is one of the most important lymphangiogenic inducers that promotes the lymphatic vessels growth and supports the survival of adult lymphatic endothelial cells. Methods. Lymphatic vessels density (LVD) and LV morphometry were digitally evaluated using D2-40. The expression of VEGF-C was also assessed using immunohistochemistry and real-time polymerase chain reaction in 6 normal oral mucosa cases and 72 cases of OSCC. The correlation between LVD and LV morphometry, VEGF-C, and lymph node metastasis was statistically assessed. Results. A positive cytoplasmic expression of VEGF-C was detected in both epithelial and connective tissue cells in 97% of OSCC, while all normal tissues reacted negatively. A greater expression of VEGF-C was associated with larger and more dilated LV and lymph node metastasis but not with LVD. Conclusion. VEGF-C is actively involved in the invasion and metastasis of OSCC via inducing morphological changes in LV. VEGF-C may be a promising target for anticancer therapy. |
| format | Article |
| id | doaj-art-c32f0ff24a4542b096366c700bda9bed |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-c32f0ff24a4542b096366c700bda9bed2025-02-03T01:00:21ZengWileyInternational Journal of Dentistry1687-87281687-87362017-01-01201710.1155/2017/72856567285656Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node MetastasisManar A. Abdul-Aziz0Amina K. Amin1Dalia H. El-Rouby2Olfat G. Shaker3Department of Oral and Maxillofacial Pathology, Faculty of Oral & Dental Medicine, Cairo University, Giza, EgyptDepartment of Oral and Maxillofacial Pathology, Faculty of Oral & Dental Medicine, Cairo University, Giza, EgyptDepartment of Oral and Maxillofacial Pathology, Faculty of Oral & Dental Medicine, Cairo University, Giza, EgyptDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, EgyptBackground. Oral squamous cell carcinoma (OSCC) is the most common oral malignancy that preferentially spreads to the cervical lymph node which, when involved, complicates the anticancer therapy and threatens the patient life. It was suggested that lymph node metastasis may be facilitated by lymphangiogenesis. VEGF-C is one of the most important lymphangiogenic inducers that promotes the lymphatic vessels growth and supports the survival of adult lymphatic endothelial cells. Methods. Lymphatic vessels density (LVD) and LV morphometry were digitally evaluated using D2-40. The expression of VEGF-C was also assessed using immunohistochemistry and real-time polymerase chain reaction in 6 normal oral mucosa cases and 72 cases of OSCC. The correlation between LVD and LV morphometry, VEGF-C, and lymph node metastasis was statistically assessed. Results. A positive cytoplasmic expression of VEGF-C was detected in both epithelial and connective tissue cells in 97% of OSCC, while all normal tissues reacted negatively. A greater expression of VEGF-C was associated with larger and more dilated LV and lymph node metastasis but not with LVD. Conclusion. VEGF-C is actively involved in the invasion and metastasis of OSCC via inducing morphological changes in LV. VEGF-C may be a promising target for anticancer therapy.http://dx.doi.org/10.1155/2017/7285656 |
| spellingShingle | Manar A. Abdul-Aziz Amina K. Amin Dalia H. El-Rouby Olfat G. Shaker Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis International Journal of Dentistry |
| title | Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis |
| title_full | Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis |
| title_fullStr | Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis |
| title_full_unstemmed | Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis |
| title_short | Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis |
| title_sort | lymphangiogenesis in oral squamous cell carcinoma correlation with vegf c expression and lymph node metastasis |
| url | http://dx.doi.org/10.1155/2017/7285656 |
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