Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model
Objective. Diabetic nephropathy (DN) is a common microvascular complication of diabetes mellitus, and insulin therapy has many side effects in the treatment of DN. Islet transplantation has emerged as a promising therapy for diabetic patients. This study was established to investigate its advantageo...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4157313 |
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| author | Yunqiang He Ziqiang Xu Mingshi Zhou Minmin Wu Xuehai Chen Silu Wang Kaiyan Qiu Yong Cai Hongxing Fu Bicheng Chen Mengtao Zhou |
| author_facet | Yunqiang He Ziqiang Xu Mingshi Zhou Minmin Wu Xuehai Chen Silu Wang Kaiyan Qiu Yong Cai Hongxing Fu Bicheng Chen Mengtao Zhou |
| author_sort | Yunqiang He |
| collection | DOAJ |
| description | Objective. Diabetic nephropathy (DN) is a common microvascular complication of diabetes mellitus, and insulin therapy has many side effects in the treatment of DN. Islet transplantation has emerged as a promising therapy for diabetic patients. This study was established to investigate its advantageous effects in a rat model of early DN. Methods. Streptozotocin was administered to the rats to induce diabetes. Twelve weeks later, the diabetic rats were divided into 3 groups: the islet-transplanted group (IT group), the insulin-treated group (IN group), and the untreated group (DN group). Renal injury and kidney structure were assessed by urinalysis and transmission electron microscopy (TEM) detection. Immunohistochemical staining and western blotting were performed to assess renal fibrosis levels. Results. The early DN features were reversed and the glomerular filtration barrier and basement membrane structures were improved at 4 weeks after islet transplantation. The urine microalbumin-to-creatinine ratio (ACR), protein-to-creatinine ratio, and mean thickness of the glomerular basement membrane (GBM) were significantly decreased in the IT group. The expression of renal fibrotic factors was also significantly decreased. Conclusions. These data suggest that early DN can be reversed after islet transplantation, and they may facilitate the development of a clinical therapeutic strategy for human diabetes mellitus. |
| format | Article |
| id | doaj-art-c323d0c45e044162991bd91b69de5abb |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-c323d0c45e044162991bd91b69de5abb2025-02-03T01:30:56ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/41573134157313Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat ModelYunqiang He0Ziqiang Xu1Mingshi Zhou2Minmin Wu3Xuehai Chen4Silu Wang5Kaiyan Qiu6Yong Cai7Hongxing Fu8Bicheng Chen9Mengtao Zhou10Key Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaDepartment of Transplantation, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, ChinaKey Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaKey Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaKey Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaKey Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaSchool of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, ChinaDepartment of Transplantation, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, ChinaSchool of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, ChinaKey Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaDepartment of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaObjective. Diabetic nephropathy (DN) is a common microvascular complication of diabetes mellitus, and insulin therapy has many side effects in the treatment of DN. Islet transplantation has emerged as a promising therapy for diabetic patients. This study was established to investigate its advantageous effects in a rat model of early DN. Methods. Streptozotocin was administered to the rats to induce diabetes. Twelve weeks later, the diabetic rats were divided into 3 groups: the islet-transplanted group (IT group), the insulin-treated group (IN group), and the untreated group (DN group). Renal injury and kidney structure were assessed by urinalysis and transmission electron microscopy (TEM) detection. Immunohistochemical staining and western blotting were performed to assess renal fibrosis levels. Results. The early DN features were reversed and the glomerular filtration barrier and basement membrane structures were improved at 4 weeks after islet transplantation. The urine microalbumin-to-creatinine ratio (ACR), protein-to-creatinine ratio, and mean thickness of the glomerular basement membrane (GBM) were significantly decreased in the IT group. The expression of renal fibrotic factors was also significantly decreased. Conclusions. These data suggest that early DN can be reversed after islet transplantation, and they may facilitate the development of a clinical therapeutic strategy for human diabetes mellitus.http://dx.doi.org/10.1155/2016/4157313 |
| spellingShingle | Yunqiang He Ziqiang Xu Mingshi Zhou Minmin Wu Xuehai Chen Silu Wang Kaiyan Qiu Yong Cai Hongxing Fu Bicheng Chen Mengtao Zhou Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model Journal of Diabetes Research |
| title | Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model |
| title_full | Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model |
| title_fullStr | Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model |
| title_full_unstemmed | Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model |
| title_short | Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model |
| title_sort | reversal of early diabetic nephropathy by islet transplantation under the kidney capsule in a rat model |
| url | http://dx.doi.org/10.1155/2016/4157313 |
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