Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’
Abstract Background Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated duri...
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2025-01-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-025-02606-9 |
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| author | Shraddha Maitra Bruce Dien Kristen Eilts Nurzhan Kuanyshev Yoel R. Cortes-Pena Yong-Su Jin Jeremy S. Guest Vijay Singh |
| author_facet | Shraddha Maitra Bruce Dien Kristen Eilts Nurzhan Kuanyshev Yoel R. Cortes-Pena Yong-Su Jin Jeremy S. Guest Vijay Singh |
| author_sort | Shraddha Maitra |
| collection | DOAJ |
| description | Abstract Background Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated during the processing of lignocellulosic biomass to produce fermentable sugars is a major challenge in developing scalable processes for second-generation drop-in fuel production. To this end, hydrolysates generated from engineered sugarcane—‘oilcane’ bagasse contain added phenolics and fatty acids that further restrict the growth of fermenting microorganisms and necessitate nutrient supplementation and/or detoxification of hydrolysate which makes the fermentation process expensive. Herein, we propose a resourceful and economical approach for growing lab and commercial strains of S. cerevisiae on unrefined cellulosic sugars aerobically and fermentatively. Results An equal ratio of hydrolysate and juice was found optimum for growth and fermentation by lab and commercial strains of Saccharomyces cerevisiae engineered for xylose fermentation. The industrial strain grew and fermented efficiently under low aeration conditions having an ethanol titer, yield, specific and volumetric productivities of 46.96 ± 0.19 g/l, 0.51 ± 0.00 g/g, 0.27 ± 0.02 g/g.h and 1.95 ± 0.01 g/l.h, respectively, while the lab strain grew better under higher aeration conditions having the ethanol titer, yield, specific and volumetric productivities of 24.93 ± 0.09, 0.27 ± 0.00 g/g, 0.17 ± 0.00 g/g.h and 1.04 ± 0.00 g/l.h, respectively. Acclimation of cultures in a blended medium significantly improved the performance of the yeast strains. Conclusions The addition of transgenic oilcane juice, which is inedible and rich in amino acids, to the hydrolysate averted the need for expensive nutrient supplementation and detoxification steps of hydrolysate. The approach provides an economical solution to reduce the cost of fermentation at an industrial scale for second-generation drop-in fuel production. Graphical Abstract |
| format | Article |
| id | doaj-art-c3114d16e0db4d9e956259282fee00e5 |
| institution | Kabale University |
| issn | 2731-3654 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj-art-c3114d16e0db4d9e956259282fee00e52025-02-02T12:12:40ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542025-01-0118111610.1186/s13068-025-02606-9Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’Shraddha Maitra0Bruce Dien1Kristen Eilts2Nurzhan Kuanyshev3Yoel R. Cortes-Pena4Yong-Su Jin5Jeremy S. Guest6Vijay Singh7Department of Agricultural and Biological Engineering, University of Illinois Urbana-ChampaignUnited States Department of Agriculture (USDA), Bioenergy Research Unit, Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR)Department of Agricultural and Biological Engineering, University of Illinois Urbana-ChampaignCarl R.Woese Institute for Genomic Biology, University of Illinois Urbana-ChampaignDepartment of Civil and Environmental Engineering, University of Illinois Urbana-ChampaignCarl R.Woese Institute for Genomic Biology, University of Illinois Urbana-ChampaignDepartment of Civil and Environmental Engineering, University of Illinois Urbana-ChampaignDepartment of Agricultural and Biological Engineering, University of Illinois Urbana-ChampaignAbstract Background Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated during the processing of lignocellulosic biomass to produce fermentable sugars is a major challenge in developing scalable processes for second-generation drop-in fuel production. To this end, hydrolysates generated from engineered sugarcane—‘oilcane’ bagasse contain added phenolics and fatty acids that further restrict the growth of fermenting microorganisms and necessitate nutrient supplementation and/or detoxification of hydrolysate which makes the fermentation process expensive. Herein, we propose a resourceful and economical approach for growing lab and commercial strains of S. cerevisiae on unrefined cellulosic sugars aerobically and fermentatively. Results An equal ratio of hydrolysate and juice was found optimum for growth and fermentation by lab and commercial strains of Saccharomyces cerevisiae engineered for xylose fermentation. The industrial strain grew and fermented efficiently under low aeration conditions having an ethanol titer, yield, specific and volumetric productivities of 46.96 ± 0.19 g/l, 0.51 ± 0.00 g/g, 0.27 ± 0.02 g/g.h and 1.95 ± 0.01 g/l.h, respectively, while the lab strain grew better under higher aeration conditions having the ethanol titer, yield, specific and volumetric productivities of 24.93 ± 0.09, 0.27 ± 0.00 g/g, 0.17 ± 0.00 g/g.h and 1.04 ± 0.00 g/l.h, respectively. Acclimation of cultures in a blended medium significantly improved the performance of the yeast strains. Conclusions The addition of transgenic oilcane juice, which is inedible and rich in amino acids, to the hydrolysate averted the need for expensive nutrient supplementation and detoxification steps of hydrolysate. The approach provides an economical solution to reduce the cost of fermentation at an industrial scale for second-generation drop-in fuel production. Graphical Abstracthttps://doi.org/10.1186/s13068-025-02606-9Fermentation media developmentFermentationDrop-in-fuelLignocellulosic hydrolysateTransgenic oilcaneC6/C5 metabolizing yeasts |
| spellingShingle | Shraddha Maitra Bruce Dien Kristen Eilts Nurzhan Kuanyshev Yoel R. Cortes-Pena Yong-Su Jin Jeremy S. Guest Vijay Singh Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ Biotechnology for Biofuels and Bioproducts Fermentation media development Fermentation Drop-in-fuel Lignocellulosic hydrolysate Transgenic oilcane C6/C5 metabolizing yeasts |
| title | Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ |
| title_full | Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ |
| title_fullStr | Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ |
| title_full_unstemmed | Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ |
| title_short | Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ |
| title_sort | resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock transgenic oilcane |
| topic | Fermentation media development Fermentation Drop-in-fuel Lignocellulosic hydrolysate Transgenic oilcane C6/C5 metabolizing yeasts |
| url | https://doi.org/10.1186/s13068-025-02606-9 |
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