Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments
Objective: To screen and identify the key active molecules, signaling pathways, and therapeutic targets of Shuxuening (SXN) injection for treating liver cirrhosis (LC) and to evaluate its therapeutic potential using a mouse model. Methods: Target genes of SXN and LC were retrieved from public databa...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754824001030 |
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| author | Qiyao Liu Tingyu Zhang Yongan Ye Xin Sun Huan Xia Xu Cao Xiaoke Li Wenying Qi Yue Chen Xiaobin Zao |
| author_facet | Qiyao Liu Tingyu Zhang Yongan Ye Xin Sun Huan Xia Xu Cao Xiaoke Li Wenying Qi Yue Chen Xiaobin Zao |
| author_sort | Qiyao Liu |
| collection | DOAJ |
| description | Objective: To screen and identify the key active molecules, signaling pathways, and therapeutic targets of Shuxuening (SXN) injection for treating liver cirrhosis (LC) and to evaluate its therapeutic potential using a mouse model. Methods: Target genes of SXN and LC were retrieved from public databases, and enrichment analysis was performed. A protein–protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and hub genes were identified using Molecular Complex Detection (MCODE). LC was induced in rats and mice via intraperitoneal injections of diethylnitrosamine and carbon tetrachloride (CCl4) for 12 weeks. Starting at week 7, SXN was administered intraperitoneally to the mice in the treatment group. Serum and liver tissues of the mice were collected for the detection of indicators, pathological staining, and expression analysis of hub targets using quantitative real-time polymerase chain reaction (qRT-PCR). Results: We identified 368 overlapping genes (OLGs) between SXN and LC targets. These OLGs were subsequently used to build a PPI network and to screen for hub genes. Enrichment analysis showed that these genes were associated with cancer-related pathways, including phosphoinositide-3-kinase/Akt and mitogen-activated protein kinase signaling and various cellular processes, such as responses to chemicals and metabolic regulation. In vivo experiments demonstrated that SXN treatment significantly improved liver function and pathology in CCl4-induced LC mice by reducing inflammation and collagen deposition. Furthermore, qRT-PCR demonstrated that SXN regulated the expression of MAPK8, AR and CASP3 in the livers of LC mice. Conclusion: This study highlighted the therapeutic effects of SXN in alleviating LC using both bioinformatics and experimental methods. The observed effect was associated with modulation of hub gene expression, particularly MAPK8, and CASP3. |
| format | Article |
| id | doaj-art-c308dca9ea664fe18dad2f839070db11 |
| institution | Kabale University |
| issn | 2095-7548 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj-art-c308dca9ea664fe18dad2f839070db112025-01-25T04:11:13ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482025-01-01121112124Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experimentsQiyao Liu0Tingyu Zhang1Yongan Ye2Xin Sun3Huan Xia4Xu Cao5Xiaoke Li6Wenying Qi7Yue Chen8Xiaobin Zao9Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China; Corresponding author.Objective: To screen and identify the key active molecules, signaling pathways, and therapeutic targets of Shuxuening (SXN) injection for treating liver cirrhosis (LC) and to evaluate its therapeutic potential using a mouse model. Methods: Target genes of SXN and LC were retrieved from public databases, and enrichment analysis was performed. A protein–protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and hub genes were identified using Molecular Complex Detection (MCODE). LC was induced in rats and mice via intraperitoneal injections of diethylnitrosamine and carbon tetrachloride (CCl4) for 12 weeks. Starting at week 7, SXN was administered intraperitoneally to the mice in the treatment group. Serum and liver tissues of the mice were collected for the detection of indicators, pathological staining, and expression analysis of hub targets using quantitative real-time polymerase chain reaction (qRT-PCR). Results: We identified 368 overlapping genes (OLGs) between SXN and LC targets. These OLGs were subsequently used to build a PPI network and to screen for hub genes. Enrichment analysis showed that these genes were associated with cancer-related pathways, including phosphoinositide-3-kinase/Akt and mitogen-activated protein kinase signaling and various cellular processes, such as responses to chemicals and metabolic regulation. In vivo experiments demonstrated that SXN treatment significantly improved liver function and pathology in CCl4-induced LC mice by reducing inflammation and collagen deposition. Furthermore, qRT-PCR demonstrated that SXN regulated the expression of MAPK8, AR and CASP3 in the livers of LC mice. Conclusion: This study highlighted the therapeutic effects of SXN in alleviating LC using both bioinformatics and experimental methods. The observed effect was associated with modulation of hub gene expression, particularly MAPK8, and CASP3.http://www.sciencedirect.com/science/article/pii/S2095754824001030Shuxuening injectionGinkgo biloba extractLiver cirrhosisNetwork pharmacologyAnimal experiments |
| spellingShingle | Qiyao Liu Tingyu Zhang Yongan Ye Xin Sun Huan Xia Xu Cao Xiaoke Li Wenying Qi Yue Chen Xiaobin Zao Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments Journal of Traditional Chinese Medical Sciences Shuxuening injection Ginkgo biloba extract Liver cirrhosis Network pharmacology Animal experiments |
| title | Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments |
| title_full | Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments |
| title_fullStr | Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments |
| title_full_unstemmed | Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments |
| title_short | Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments |
| title_sort | determining the mechanism of shuxuening injection against liver cirrhosis through network pharmacology and animal experiments |
| topic | Shuxuening injection Ginkgo biloba extract Liver cirrhosis Network pharmacology Animal experiments |
| url | http://www.sciencedirect.com/science/article/pii/S2095754824001030 |
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