Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD
Background. Optimal management of pediatric patients with inflammatory bowel disease (IBD) requires early diagnosis. Aim of the study is to compare fecal lactoferrin (FL) as biomarker of intestinal inflammation to CRP in pediatric patients with new-onset IBD. Methods. FL was measured by ELISA in sto...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2015/578527 |
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| author | Stephan Buderus James H. Boone Michael J. Lentze |
| author_facet | Stephan Buderus James H. Boone Michael J. Lentze |
| author_sort | Stephan Buderus |
| collection | DOAJ |
| description | Background. Optimal management of pediatric patients with inflammatory bowel disease (IBD) requires early diagnosis. Aim of the study is to compare fecal lactoferrin (FL) as biomarker of intestinal inflammation to CRP in pediatric patients with new-onset IBD. Methods. FL was measured by ELISA in stool specimens collected prior to endoscopy for IBD (IBD-SCAN; TechLab, Blacksburg; normal < 7.3 µg/g feces). CRP was detected in serum (normal < 5 mg/L). Three patient groups were determined: n=21 (mean age 13.2) with Crohn’s disease (CD), n=15 (mean age 10.9) with ulcerative colitis (UC), and n=20 (mean age 11.9) in whom IBD was ruled out. In CD patients the endoscopic severity score SES-CD was correlated with the FL levels. Results. (Mean ± SEM). CRP levels were 27.18 ± 4.2 for CD-cases, 20.8 ± 9.5 for UC, and 0.24 ± 0.06 for non-IBD patients. FL levels were 313.6 ± 46.4 in CD, 370.7 ± 46.9 in UC, and 1.3 ± 0.5 in non-IBD patients. Sensitivity of CRP to detect IBD was 75% with specificity of 100%, positive predictive value of 100%, and negative predictive value of 69%. Sensitivity of FL was 100% with specificity of 95%, positive predictive value of 97.3%, and negative predictive value of 100%. In CD, FL levels correlated positively (R2=0.42) with disease severity as judged by the SES-CD. Conclusions. Elevated FL corresponds to intestinal inflammation, even in patients with normal CRP. With high probability, normal FL excludes intestinal inflammation. |
| format | Article |
| id | doaj-art-c303c23402e042719f479a11495ad24c |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | Gastroenterology Research and Practice |
| spelling | doaj-art-c303c23402e042719f479a11495ad24c2025-02-03T01:24:07ZengWileyGastroenterology Research and Practice1687-61211687-630X2015-01-01201510.1155/2015/578527578527Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBDStephan Buderus0James H. Boone1Michael J. Lentze2Department of Pediatrics, St. Marien-Hospital, GFO-Kliniken Bonn, Robert-Koch-Straße 1, 53115 Bonn, GermanyTechLab Inc., Blacksburg, VA 24060, USADepartment of Pediatrics, University Children’s Hospital, Adenauerallee 119, 53113 Bonn, GermanyBackground. Optimal management of pediatric patients with inflammatory bowel disease (IBD) requires early diagnosis. Aim of the study is to compare fecal lactoferrin (FL) as biomarker of intestinal inflammation to CRP in pediatric patients with new-onset IBD. Methods. FL was measured by ELISA in stool specimens collected prior to endoscopy for IBD (IBD-SCAN; TechLab, Blacksburg; normal < 7.3 µg/g feces). CRP was detected in serum (normal < 5 mg/L). Three patient groups were determined: n=21 (mean age 13.2) with Crohn’s disease (CD), n=15 (mean age 10.9) with ulcerative colitis (UC), and n=20 (mean age 11.9) in whom IBD was ruled out. In CD patients the endoscopic severity score SES-CD was correlated with the FL levels. Results. (Mean ± SEM). CRP levels were 27.18 ± 4.2 for CD-cases, 20.8 ± 9.5 for UC, and 0.24 ± 0.06 for non-IBD patients. FL levels were 313.6 ± 46.4 in CD, 370.7 ± 46.9 in UC, and 1.3 ± 0.5 in non-IBD patients. Sensitivity of CRP to detect IBD was 75% with specificity of 100%, positive predictive value of 100%, and negative predictive value of 69%. Sensitivity of FL was 100% with specificity of 95%, positive predictive value of 97.3%, and negative predictive value of 100%. In CD, FL levels correlated positively (R2=0.42) with disease severity as judged by the SES-CD. Conclusions. Elevated FL corresponds to intestinal inflammation, even in patients with normal CRP. With high probability, normal FL excludes intestinal inflammation.http://dx.doi.org/10.1155/2015/578527 |
| spellingShingle | Stephan Buderus James H. Boone Michael J. Lentze Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD Gastroenterology Research and Practice |
| title | Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD |
| title_full | Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD |
| title_fullStr | Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD |
| title_full_unstemmed | Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD |
| title_short | Fecal Lactoferrin: Reliable Biomarker for Intestinal Inflammation in Pediatric IBD |
| title_sort | fecal lactoferrin reliable biomarker for intestinal inflammation in pediatric ibd |
| url | http://dx.doi.org/10.1155/2015/578527 |
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