Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats
Acute lung injury (ALI) induced by intestinal ischemia/reperfusion (II/R) has high incidence and mortality, in which IL-1β was essential for the full development of ALI. However, the detailed regulating mechanism for this phenomenon remains to be unclear. The purpose of this study was to investigate...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9348037 |
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| author | De-Yi Zheng Min Zhou Jiao Jin Mu He Yi Wang Jiao Du Xiang-Yang Xiao Ping-Yang Li Ai-Zhu Ye Jia Liu Ting-Hua Wang |
| author_facet | De-Yi Zheng Min Zhou Jiao Jin Mu He Yi Wang Jiao Du Xiang-Yang Xiao Ping-Yang Li Ai-Zhu Ye Jia Liu Ting-Hua Wang |
| author_sort | De-Yi Zheng |
| collection | DOAJ |
| description | Acute lung injury (ALI) induced by intestinal ischemia/reperfusion (II/R) has high incidence and mortality, in which IL-1β was essential for the full development of ALI. However, the detailed regulating mechanism for this phenomenon remains to be unclear. The purpose of this study was to investigate whether inhibition of P38 MAPK could downregulate the expression of IL-1β to protect lung from acute injury in II/R rats. Here, we found that the level of pulmonary edema at 16 hours after operation (hpo) was obviously enhanced compared to that in 8hpo and sham groups. Immunofluorescent staining demonstrated that IL-1β and P38 MAPK were detected in lung tissues. And rats with II/R have the highest translation level for IL-1β and phosphorylation of P38 MAPK in lung tissues at 16hpo compared with 8hpo and sham groups. Moreover, administration of SB239063, an inhibitor of P38 α and β, could effectively downregulate the expressions of IL-1β and protects lung tissues from injury in II/R rats. Our findings indicate that the inhibition of P38 α and β may downregulate the expression of IL-1β to protect lung from acute injury in II/R, which could be used as a potential target for reducing ALI induced by II/R in the future clinical trial. |
| format | Article |
| id | doaj-art-c2fc8691e7c445cd9859343fb106f491 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c2fc8691e7c445cd9859343fb106f4912025-02-03T01:02:46ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/93480379348037Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion RatsDe-Yi Zheng0Min Zhou1Jiao Jin2Mu He3Yi Wang4Jiao Du5Xiang-Yang Xiao6Ping-Yang Li7Ai-Zhu Ye8Jia Liu9Ting-Hua Wang10Department of Burn and Plastic Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, ChinaInstitute of Neuroscience, Kunming Medical University, Kunming 650031, ChinaDepartment of Pediatrics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Burn and Plastic Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, ChinaDepartment of Burn and Plastic Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, ChinaDepartment of Burn and Plastic Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, ChinaDepartment of Burn and Plastic Surgery, Guizhou Provincial People’s Hospital, Guiyang 550002, ChinaDepartment of Pediatrics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, ChinaInstitute of Neuroscience, Kunming Medical University, Kunming 650031, ChinaInstitute of Neuroscience, Kunming Medical University, Kunming 650031, ChinaAcute lung injury (ALI) induced by intestinal ischemia/reperfusion (II/R) has high incidence and mortality, in which IL-1β was essential for the full development of ALI. However, the detailed regulating mechanism for this phenomenon remains to be unclear. The purpose of this study was to investigate whether inhibition of P38 MAPK could downregulate the expression of IL-1β to protect lung from acute injury in II/R rats. Here, we found that the level of pulmonary edema at 16 hours after operation (hpo) was obviously enhanced compared to that in 8hpo and sham groups. Immunofluorescent staining demonstrated that IL-1β and P38 MAPK were detected in lung tissues. And rats with II/R have the highest translation level for IL-1β and phosphorylation of P38 MAPK in lung tissues at 16hpo compared with 8hpo and sham groups. Moreover, administration of SB239063, an inhibitor of P38 α and β, could effectively downregulate the expressions of IL-1β and protects lung tissues from injury in II/R rats. Our findings indicate that the inhibition of P38 α and β may downregulate the expression of IL-1β to protect lung from acute injury in II/R, which could be used as a potential target for reducing ALI induced by II/R in the future clinical trial.http://dx.doi.org/10.1155/2016/9348037 |
| spellingShingle | De-Yi Zheng Min Zhou Jiao Jin Mu He Yi Wang Jiao Du Xiang-Yang Xiao Ping-Yang Li Ai-Zhu Ye Jia Liu Ting-Hua Wang Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats Mediators of Inflammation |
| title | Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats |
| title_full | Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats |
| title_fullStr | Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats |
| title_full_unstemmed | Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats |
| title_short | Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats |
| title_sort | inhibition of p38 mapk downregulates the expression of il 1β to protect lung from acute injury in intestinal ischemia reperfusion rats |
| url | http://dx.doi.org/10.1155/2016/9348037 |
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