Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion
Polycaprolactone/polyurethane (PCL/PU) fibrous scaffold was loaded with conjugated linoleic acid (CLA) by electrospinning method to improve the hemocompatibility of the polymeric surface. Fourier Transform Infrared Spectroscopy (FT-IR) analysis and Scanning Electron Microscopy (SEM) observation were...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2017/5690625 |
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| author | Ho Hieu Minh Nguyen Thi Hiep Nguyen Dai Hai Vo Van Toi |
| author_facet | Ho Hieu Minh Nguyen Thi Hiep Nguyen Dai Hai Vo Van Toi |
| author_sort | Ho Hieu Minh |
| collection | DOAJ |
| description | Polycaprolactone/polyurethane (PCL/PU) fibrous scaffold was loaded with conjugated linoleic acid (CLA) by electrospinning method to improve the hemocompatibility of the polymeric surface. Fourier Transform Infrared Spectroscopy (FT-IR) analysis and Scanning Electron Microscopy (SEM) observation were employed to characterize the chemical structure and the changing morphology of electrospun PCL/PU and PCL/PU loaded with CLA (PCL/PU-CLA) scaffolds. Platelet adhesion and whole blood clot formation tests were used to evaluate the effect of CLA on antithrombotic property of PCL/PU-CLA scaffold. Endothelial cells (EC) were also seeded on the scaffold to examine the difference in the morphology of EC layer and platelet attachment with and without the presence of CLA. SEM results showed that CLA supported the spreading and proliferation of EC and PCL/PU-CLA surface induced lower platelet adhesion as well as attachment of other blood cells compared to the PCL/PU one. These results suggest that electrospinning method can successfully combine the antiplatelet effects of CLA to improve hemocompatibility of PCL/PU scaffolds for applications in artificial blood vessels. |
| format | Article |
| id | doaj-art-c2f1dcc50e7447849f8ec98178f33c53 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-c2f1dcc50e7447849f8ec98178f33c532025-02-03T01:09:40ZengWileyInternational Journal of Biomaterials1687-87871687-87952017-01-01201710.1155/2017/56906255690625Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet AdhesionHo Hieu Minh0Nguyen Thi Hiep1Nguyen Dai Hai2Vo Van Toi3Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University of Vietnam National Universities, Ho Chi Minh City 700000, VietnamTissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University of Vietnam National Universities, Ho Chi Minh City 700000, VietnamInstitute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi, District 1, Ho Chi Minh City, VietnamTissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University of Vietnam National Universities, Ho Chi Minh City 700000, VietnamPolycaprolactone/polyurethane (PCL/PU) fibrous scaffold was loaded with conjugated linoleic acid (CLA) by electrospinning method to improve the hemocompatibility of the polymeric surface. Fourier Transform Infrared Spectroscopy (FT-IR) analysis and Scanning Electron Microscopy (SEM) observation were employed to characterize the chemical structure and the changing morphology of electrospun PCL/PU and PCL/PU loaded with CLA (PCL/PU-CLA) scaffolds. Platelet adhesion and whole blood clot formation tests were used to evaluate the effect of CLA on antithrombotic property of PCL/PU-CLA scaffold. Endothelial cells (EC) were also seeded on the scaffold to examine the difference in the morphology of EC layer and platelet attachment with and without the presence of CLA. SEM results showed that CLA supported the spreading and proliferation of EC and PCL/PU-CLA surface induced lower platelet adhesion as well as attachment of other blood cells compared to the PCL/PU one. These results suggest that electrospinning method can successfully combine the antiplatelet effects of CLA to improve hemocompatibility of PCL/PU scaffolds for applications in artificial blood vessels.http://dx.doi.org/10.1155/2017/5690625 |
| spellingShingle | Ho Hieu Minh Nguyen Thi Hiep Nguyen Dai Hai Vo Van Toi Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion International Journal of Biomaterials |
| title | Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion |
| title_full | Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion |
| title_fullStr | Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion |
| title_full_unstemmed | Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion |
| title_short | Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion |
| title_sort | fabrication of polycaprolactone polyurethane loading conjugated linoleic acid and its antiplatelet adhesion |
| url | http://dx.doi.org/10.1155/2017/5690625 |
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