Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress
Morchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells f...
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| Format: | Article |
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2019-08-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.journalssystem.com/pjfns/The-cytoprotective-effect-of-Morchella-esculenta-protein-hydrolysate-and-it-s-derivative,110134,0,2.html |
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| author | Qiang Zhang Caie Wu Yujun Sun Tingting Li Gongjian Fan |
| author_facet | Qiang Zhang Caie Wu Yujun Sun Tingting Li Gongjian Fan |
| author_sort | Qiang Zhang |
| collection | DOAJ |
| description | Morchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells from H2O2-induced oxidative injury and explored the potential mechanisms of protection. The results showed that, under H2O2 stress, both MPH and G-MPH significantly increased cell viability, suppressed intracellular ROS and MDA production, increased cellular antioxidant capacity, and activated Nrf2 signaling pathway. More importantly, MPH and G-MPH significantly inhibited the H2O2-induced apoptosis via restoring the loss of mitochondrial membrane potential and regulating the protein expressions of Bax, Bcl-2, and caspase-3. These data indicate that MPH and G-MPH can protect Caco-2 cells against oxidative injury by improving cellular antioxidant responses and inhibiting apoptosis. Therefore, MPH and G-MPH can have a broad application potential as promising ingredients of nutraceutical products or functional foods. |
| format | Article |
| id | doaj-art-c2bbc52c32df4bafab0c10c9a5c7e2f1 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-c2bbc52c32df4bafab0c10c9a5c7e2f12025-02-02T07:32:37ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072019-08-0169325526510.31883/pjfns/110134110134Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative StressQiang Zhang0Caie Wu1Yujun Sun2Tingting Li3Gongjian Fan4College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Life and Health Sciences, Anhui Science and Technology University, Fengyang 233100, ChinaCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaMorchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells from H2O2-induced oxidative injury and explored the potential mechanisms of protection. The results showed that, under H2O2 stress, both MPH and G-MPH significantly increased cell viability, suppressed intracellular ROS and MDA production, increased cellular antioxidant capacity, and activated Nrf2 signaling pathway. More importantly, MPH and G-MPH significantly inhibited the H2O2-induced apoptosis via restoring the loss of mitochondrial membrane potential and regulating the protein expressions of Bax, Bcl-2, and caspase-3. These data indicate that MPH and G-MPH can protect Caco-2 cells against oxidative injury by improving cellular antioxidant responses and inhibiting apoptosis. Therefore, MPH and G-MPH can have a broad application potential as promising ingredients of nutraceutical products or functional foods.http://www.journalssystem.com/pjfns/The-cytoprotective-effect-of-Morchella-esculenta-protein-hydrolysate-and-it-s-derivative,110134,0,2.htmlmorchella esculentaprotein hydrolysatemaillard reactionoxidative stresscytoprotective effect |
| spellingShingle | Qiang Zhang Caie Wu Yujun Sun Tingting Li Gongjian Fan Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress Polish Journal of Food and Nutrition Sciences morchella esculenta protein hydrolysate maillard reaction oxidative stress cytoprotective effect |
| title | Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress |
| title_full | Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress |
| title_fullStr | Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress |
| title_full_unstemmed | Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress |
| title_short | Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress |
| title_sort | cytoprotective effect of morchella esculenta protein hydrolysate and its derivative against h2o2 induced oxidative stress |
| topic | morchella esculenta protein hydrolysate maillard reaction oxidative stress cytoprotective effect |
| url | http://www.journalssystem.com/pjfns/The-cytoprotective-effect-of-Morchella-esculenta-protein-hydrolysate-and-it-s-derivative,110134,0,2.html |
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