Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum
One hundred and eighty-six endophytic actinomycetes strains isolated from nine kinds of plants were screened for chitinase production by showing the transparent zone on the selective media containing chitin, and a high-yield strain GKSHJA of chitinase isolated from the stem of Polygonatum sibirium w...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2010-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.06.004 |
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| author | GAO Xiao-ning Gulpiye WANG Mei-ying HUANG Li-li TU Xuan KANG Zhen-sheng |
| author_facet | GAO Xiao-ning Gulpiye WANG Mei-ying HUANG Li-li TU Xuan KANG Zhen-sheng |
| author_sort | GAO Xiao-ning |
| collection | DOAJ |
| description | One hundred and eighty-six endophytic actinomycetes strains isolated from nine kinds of plants were screened for chitinase production by showing the transparent zone on the selective media containing chitin, and a high-yield strain GKSHJA of chitinase isolated from the stem of Polygonatum sibirium was obtained, whose antifungal activity on some common plant pathogens was evaluated by using liquid shake culture. The results showed that strain GKSHJA had high inhibition efficiency not only on Sclerotinia sclerotiorum with 85.4% inhibition rate to mycelial growth, but also on other 10 kinds of phytopathogens. The strain GKSHJA was identified as Streptomyces longisporoflavus based on morphological, physiological and biochemical, and cytochemical characteristics. The medium with initial pH 6, containing 1% floury chitin as carbon source and 0.30% beef extract as nitrogen source, incubated at 32℃ for 6 d with 150 r·min<sup>-1</sup>, was recommended for chitinase production of the strain GKSHJA. |
| format | Article |
| id | doaj-art-c2bb98b807f04599b7a5cc1978a89b51 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2010-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c2bb98b807f04599b7a5cc1978a89b512025-08-20T02:47:44ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552010-11-013661562210.3785/j.issn.1008-9209.2010.06.00410089209Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorumGAO Xiao-ningGulpiyeWANG Mei-yingHUANG Li-liTU XuanKANG Zhen-shengOne hundred and eighty-six endophytic actinomycetes strains isolated from nine kinds of plants were screened for chitinase production by showing the transparent zone on the selective media containing chitin, and a high-yield strain GKSHJA of chitinase isolated from the stem of Polygonatum sibirium was obtained, whose antifungal activity on some common plant pathogens was evaluated by using liquid shake culture. The results showed that strain GKSHJA had high inhibition efficiency not only on Sclerotinia sclerotiorum with 85.4% inhibition rate to mycelial growth, but also on other 10 kinds of phytopathogens. The strain GKSHJA was identified as Streptomyces longisporoflavus based on morphological, physiological and biochemical, and cytochemical characteristics. The medium with initial pH 6, containing 1% floury chitin as carbon source and 0.30% beef extract as nitrogen source, incubated at 32℃ for 6 d with 150 r·min<sup>-1</sup>, was recommended for chitinase production of the strain GKSHJA.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.06.004endophytic actinomycetechitinase<italic>Sclerotinia sclerotiorum</italic>antifungal activityfermentation conditions |
| spellingShingle | GAO Xiao-ning Gulpiye WANG Mei-ying HUANG Li-li TU Xuan KANG Zhen-sheng Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum 浙江大学学报. 农业与生命科学版 endophytic actinomycete chitinase <italic>Sclerotinia sclerotiorum</italic> antifungal activity fermentation conditions |
| title | Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum |
| title_full | Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum |
| title_fullStr | Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum |
| title_full_unstemmed | Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum |
| title_short | Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum |
| title_sort | screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against sclerotinia sclerotiorum |
| topic | endophytic actinomycete chitinase <italic>Sclerotinia sclerotiorum</italic> antifungal activity fermentation conditions |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.06.004 |
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