Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury
Purpose. Onset of transfusion-related acute lung injury (TRALI) is suggested to be a threshold-event. Data is lacking on the relation between titer of antibodies infused and onset of TRALI. We determined whether onset of TRALI is dependent on the titer of MHC-I antibodies infused in a combined model...
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| Format: | Article |
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Wiley
2012-01-01
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| Series: | Critical Care Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/720950 |
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| author | A. P. J. Vlaar M. T. Kuipers J. J. Hofstra E. K. Wolthuis C. W. Wieland J. J. T. H. Roelofs L. Boon M. J. Schultz R. Lutter N. P. Juffermans |
| author_facet | A. P. J. Vlaar M. T. Kuipers J. J. Hofstra E. K. Wolthuis C. W. Wieland J. J. T. H. Roelofs L. Boon M. J. Schultz R. Lutter N. P. Juffermans |
| author_sort | A. P. J. Vlaar |
| collection | DOAJ |
| description | Purpose. Onset of transfusion-related acute lung injury (TRALI) is suggested to be a threshold-event. Data is lacking on the relation between titer of antibodies infused and onset of TRALI. We determined whether onset of TRALI is dependent on the titer of MHC-I antibodies infused in a combined model of ventilator-induced lung injury and antibody-induced TRALl. Methods. BALB/c mice were ventilated for five hours with low (7.5 ml/kg) or high (15 ml/kg) tidal volume. After three hours of MV, TRALI was induced by infusion of 0.5 mg/kg, 2.0 mg/kg or 4.5 mg/kg MHC-I antibodies. Control animals received vehicle. After five hours of MV, animals were sacrificed. Results. MV with high tidal volumes resulted in increased levels of all markers of lung injury compared to animals ventilated with low tidal MV. In ventilator-induced lung injury, infusion of 4.5 mg/kg of antibodies further increased pulmonary wet-to-dry ratio, pulmonary neutrophil influx and pulmonary KC levels, whereas infusion of lower dose of antibodies did not augment lung injury. In contrast, mice ventilated with low tidal volumes did not develop lung injury, irrespective of the dose of antibody used. Conclusions. In the presence of injurious MV, onset of TRALI depends on the titer of antibodies infused. |
| format | Article |
| id | doaj-art-c2baf41922c14bb485e35e586c4c435d |
| institution | Kabale University |
| issn | 2090-1305 2090-1313 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Critical Care Research and Practice |
| spelling | doaj-art-c2baf41922c14bb485e35e586c4c435d2025-02-03T01:31:52ZengWileyCritical Care Research and Practice2090-13052090-13132012-01-01201210.1155/2012/720950720950Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung InjuryA. P. J. Vlaar0M. T. Kuipers1J. J. Hofstra2E. K. Wolthuis3C. W. Wieland4J. J. T. H. Roelofs5L. Boon6M. J. Schultz7R. Lutter8N. P. Juffermans9Department of Intensive Care Medicine, Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsDepartment of Intensive Care Medicine, Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsLaboratory of Experimental Intensive Care and Anesthesiology (LEICA), Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsLaboratory of Experimental Intensive Care and Anesthesiology (LEICA), Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsLaboratory of Experimental Intensive Care and Anesthesiology (LEICA), Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsDepartment of Pathology, Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsBioceros B.V., 3584 CM Utrecht, The NetherlandsDepartment of Intensive Care Medicine, Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsDepartments of Respiratory Medicine and Experimental Immunology, Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsDepartment of Intensive Care Medicine, Academic Medical Center, 1105 AZ Amsterdam, The NetherlandsPurpose. Onset of transfusion-related acute lung injury (TRALI) is suggested to be a threshold-event. Data is lacking on the relation between titer of antibodies infused and onset of TRALI. We determined whether onset of TRALI is dependent on the titer of MHC-I antibodies infused in a combined model of ventilator-induced lung injury and antibody-induced TRALl. Methods. BALB/c mice were ventilated for five hours with low (7.5 ml/kg) or high (15 ml/kg) tidal volume. After three hours of MV, TRALI was induced by infusion of 0.5 mg/kg, 2.0 mg/kg or 4.5 mg/kg MHC-I antibodies. Control animals received vehicle. After five hours of MV, animals were sacrificed. Results. MV with high tidal volumes resulted in increased levels of all markers of lung injury compared to animals ventilated with low tidal MV. In ventilator-induced lung injury, infusion of 4.5 mg/kg of antibodies further increased pulmonary wet-to-dry ratio, pulmonary neutrophil influx and pulmonary KC levels, whereas infusion of lower dose of antibodies did not augment lung injury. In contrast, mice ventilated with low tidal volumes did not develop lung injury, irrespective of the dose of antibody used. Conclusions. In the presence of injurious MV, onset of TRALI depends on the titer of antibodies infused.http://dx.doi.org/10.1155/2012/720950 |
| spellingShingle | A. P. J. Vlaar M. T. Kuipers J. J. Hofstra E. K. Wolthuis C. W. Wieland J. J. T. H. Roelofs L. Boon M. J. Schultz R. Lutter N. P. Juffermans Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury Critical Care Research and Practice |
| title | Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury |
| title_full | Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury |
| title_fullStr | Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury |
| title_full_unstemmed | Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury |
| title_short | Mechanical Ventilation and the Titer of Antibodies as Risk Factors for the Development of Transfusion-Related Lung Injury |
| title_sort | mechanical ventilation and the titer of antibodies as risk factors for the development of transfusion related lung injury |
| url | http://dx.doi.org/10.1155/2012/720950 |
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