Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles
Alzheimer’s disease is a challenge in modern healthcare due to its complex etiology and increasing prevalence. Despite advances, further understanding of Alzheimer’s disease pathophysiology is needed, particularly the role of Aβ neurotoxic peptide. Fourier transform infrared spectroscopy (FTIR) has...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/1420-3049/30/2/258 |
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| author | Margarida Vaz Tânia Soares Martins Kevin Leandro Luís Pereira de Almeida Odete A. B. da Cruz e Silva Alexandra Nunes Ana Gabriela Henriques |
| author_facet | Margarida Vaz Tânia Soares Martins Kevin Leandro Luís Pereira de Almeida Odete A. B. da Cruz e Silva Alexandra Nunes Ana Gabriela Henriques |
| author_sort | Margarida Vaz |
| collection | DOAJ |
| description | Alzheimer’s disease is a challenge in modern healthcare due to its complex etiology and increasing prevalence. Despite advances, further understanding of Alzheimer’s disease pathophysiology is needed, particularly the role of Aβ neurotoxic peptide. Fourier transform infrared spectroscopy (FTIR) has shown potential as a screening tool for several pathologies, including Alzheimer’s disease. Nonetheless, limited research has explored Aβ direct effects on neurons and extracellular vesicles metabolic profiles. Hence, this study aims to investigate Aβ impact on the spectroscopic profiles of neuronal-like cells (N2a) and N2a-derived extracellular vesicles, employing FTIR spectroscopy and focusing on the 1280–900 cm<sup>−1</sup> region. A comprehensive analysis of spectral data was carried out, including multivariate partial least squares (PLS) analysis and peak intensities analysis. PLS analysis revealed moderate to strong correlations within this spectral region for both N2a and N2a-derived extracellular vesicles. The peak intensity analysis revealed additional peaks with significant differences in EVs’ spectra relative to N2a, following Aβ treatment. Specifically, Aβ seems to cause alterations in protein phosphorylation and in the nucleic acids content of extracellular vesicles. These findings support that Aβ’s role in Alzheimer’s disease pathology may be mediated by extracellular vesicles and highlight FTIR’s potential for advancing Alzheimer’s disease research and clinical applications. |
| format | Article |
| id | doaj-art-c291834a484b4589a3b8692456fdb2a0 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-c291834a484b4589a3b8692456fdb2a02025-01-24T13:43:17ZengMDPI AGMolecules1420-30492025-01-0130225810.3390/molecules30020258Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular VesiclesMargarida Vaz0Tânia Soares Martins1Kevin Leandro2Luís Pereira de Almeida3Odete A. B. da Cruz e Silva4Alexandra Nunes5Ana Gabriela Henriques6Neuroscience and Signalling Group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugalNeuroscience and Signalling Group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugalCenter for Neuroscience and Cell Biology, Faculty of Pharmacy, University of Coimbra, 3004-504 Coimbra, PortugalCenter for Neuroscience and Cell Biology, Faculty of Pharmacy, University of Coimbra, 3004-504 Coimbra, PortugalNeuroscience and Signalling Group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugalInstitute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugalNeuroscience and Signalling Group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugalAlzheimer’s disease is a challenge in modern healthcare due to its complex etiology and increasing prevalence. Despite advances, further understanding of Alzheimer’s disease pathophysiology is needed, particularly the role of Aβ neurotoxic peptide. Fourier transform infrared spectroscopy (FTIR) has shown potential as a screening tool for several pathologies, including Alzheimer’s disease. Nonetheless, limited research has explored Aβ direct effects on neurons and extracellular vesicles metabolic profiles. Hence, this study aims to investigate Aβ impact on the spectroscopic profiles of neuronal-like cells (N2a) and N2a-derived extracellular vesicles, employing FTIR spectroscopy and focusing on the 1280–900 cm<sup>−1</sup> region. A comprehensive analysis of spectral data was carried out, including multivariate partial least squares (PLS) analysis and peak intensities analysis. PLS analysis revealed moderate to strong correlations within this spectral region for both N2a and N2a-derived extracellular vesicles. The peak intensity analysis revealed additional peaks with significant differences in EVs’ spectra relative to N2a, following Aβ treatment. Specifically, Aβ seems to cause alterations in protein phosphorylation and in the nucleic acids content of extracellular vesicles. These findings support that Aβ’s role in Alzheimer’s disease pathology may be mediated by extracellular vesicles and highlight FTIR’s potential for advancing Alzheimer’s disease research and clinical applications.https://www.mdpi.com/1420-3049/30/2/258FTIRAβAlzheimer’s diseaseextracellular vesiclesNeuro-2a cells |
| spellingShingle | Margarida Vaz Tânia Soares Martins Kevin Leandro Luís Pereira de Almeida Odete A. B. da Cruz e Silva Alexandra Nunes Ana Gabriela Henriques Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles Molecules FTIR Aβ Alzheimer’s disease extracellular vesicles Neuro-2a cells |
| title | Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles |
| title_full | Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles |
| title_fullStr | Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles |
| title_full_unstemmed | Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles |
| title_short | Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles |
| title_sort | fourier transform infrared spectroscopy analysis as a tool to address aβ impact on extracellular vesicles |
| topic | FTIR Aβ Alzheimer’s disease extracellular vesicles Neuro-2a cells |
| url | https://www.mdpi.com/1420-3049/30/2/258 |
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