Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) pati...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/2131787 |
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| author | Roghayeh Nouri Alka Hasani Kourosh Masnadi Shirazi Mohammad Reza Alivand Bita Sepehri Simin Sotoudeh Fatemeh Hemmati Afshin Fattahzadeh Babak Abdinia Mohammad Ahangarzadeh Rezaee |
| author_facet | Roghayeh Nouri Alka Hasani Kourosh Masnadi Shirazi Mohammad Reza Alivand Bita Sepehri Simin Sotoudeh Fatemeh Hemmati Afshin Fattahzadeh Babak Abdinia Mohammad Ahangarzadeh Rezaee |
| author_sort | Roghayeh Nouri |
| collection | DOAJ |
| description | Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) patients and control group. At two referral university-affiliated hospitals in northwest Iran, 100 patients, 50 with CRC and 50 without, were studied over the course of a year. Fresh biopsy specimens were used to identify mucosa-associated E. coli isolates after dithiothreitol mucolysis. To classify the E. coli strains, ten colonies per sample were typed using enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR). The strains were classified into phylogroups using the quadruplex PCR method. The PCR method was used to examine for the presence of cyclomodulin, bfp, stx1, stx2, and eae-encoding genes. The strains were tested for biofilm formation using the microtiter plate assay. CRC patients had more mucosa-associated E. coli than the control group (p<0.05). Enteropathogenic Escherichia coli (EPEC) was also found in 23% of CRC strains and 7.1% of control strains (p<0.05). Phylogroup A was predominant in control group specimens, while E. coli isolates from CRC patients belonged most frequently to phylogroups D and B2. Furthermore, the frequency of cyclomodulin-encoding genes in the CRC patients was significantly higher than the control group. Around 36.9% of E. coli strains from CRC samples were able to form biofilms, compared to 16.6% E. coli strains from the control group (p<0.05). Noticeably, cyclomodulin-positive strains were more likely to form biofilm in comparison to cyclomodulin-negative strains (p<0.05). In conclusion, mucosa-associated E. coli especially cyclomodulin-positive isolates from B2 and D phylogroups possessing biofilm-producing capacity colonize the gut mucosa of CRC patients. |
| format | Article |
| id | doaj-art-c272939ecd014d0b93835723f23d007b |
| institution | Kabale University |
| issn | 1918-1493 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-c272939ecd014d0b93835723f23d007b2025-02-03T01:26:24ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1918-14932021-01-01202110.1155/2021/2131787Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing FeaturesRoghayeh Nouri0Alka Hasani1Kourosh Masnadi Shirazi2Mohammad Reza Alivand3Bita Sepehri4Simin Sotoudeh5Fatemeh Hemmati6Afshin Fattahzadeh7Babak Abdinia8Mohammad Ahangarzadeh Rezaee9Student Research CommitteeInfectious and Tropical Diseases Research CenterLiver and Gastrointestinal Diseases Research CenterDepartment of Medical GeneticsLiver and Gastrointestinal Diseases Research CenterLiver and Gastrointestinal Diseases Research CenterStudent Research CommitteeLiver and Gastrointestinal Diseases Research CenterPediatric Health Research CenterInfectious and Tropical Diseases Research CenterAccumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) patients and control group. At two referral university-affiliated hospitals in northwest Iran, 100 patients, 50 with CRC and 50 without, were studied over the course of a year. Fresh biopsy specimens were used to identify mucosa-associated E. coli isolates after dithiothreitol mucolysis. To classify the E. coli strains, ten colonies per sample were typed using enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR). The strains were classified into phylogroups using the quadruplex PCR method. The PCR method was used to examine for the presence of cyclomodulin, bfp, stx1, stx2, and eae-encoding genes. The strains were tested for biofilm formation using the microtiter plate assay. CRC patients had more mucosa-associated E. coli than the control group (p<0.05). Enteropathogenic Escherichia coli (EPEC) was also found in 23% of CRC strains and 7.1% of control strains (p<0.05). Phylogroup A was predominant in control group specimens, while E. coli isolates from CRC patients belonged most frequently to phylogroups D and B2. Furthermore, the frequency of cyclomodulin-encoding genes in the CRC patients was significantly higher than the control group. Around 36.9% of E. coli strains from CRC samples were able to form biofilms, compared to 16.6% E. coli strains from the control group (p<0.05). Noticeably, cyclomodulin-positive strains were more likely to form biofilm in comparison to cyclomodulin-negative strains (p<0.05). In conclusion, mucosa-associated E. coli especially cyclomodulin-positive isolates from B2 and D phylogroups possessing biofilm-producing capacity colonize the gut mucosa of CRC patients.http://dx.doi.org/10.1155/2021/2131787 |
| spellingShingle | Roghayeh Nouri Alka Hasani Kourosh Masnadi Shirazi Mohammad Reza Alivand Bita Sepehri Simin Sotoudeh Fatemeh Hemmati Afshin Fattahzadeh Babak Abdinia Mohammad Ahangarzadeh Rezaee Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features |
| title_full | Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features |
| title_fullStr | Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features |
| title_full_unstemmed | Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features |
| title_short | Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features |
| title_sort | mucosa associated escherichia coli in colorectal cancer patients and control subjects variations in the prevalence and attributing features |
| url | http://dx.doi.org/10.1155/2021/2131787 |
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