miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema
Smoking is an important factor in the pathogenesis of chronic obstructive pulmonary disease (COPD), which is commonly characterised by cellular senescence and inflammation. Recently, miR-200b has emerged as an important target to cure lung disease; however, the function of miR-200b in reducing cellu...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1725029 |
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| author | Zhiming Shen Wenting Xuan Huanhuan Wang Fei Sun Chengxin Zhang Qian Gong Shenglin Ge |
| author_facet | Zhiming Shen Wenting Xuan Huanhuan Wang Fei Sun Chengxin Zhang Qian Gong Shenglin Ge |
| author_sort | Zhiming Shen |
| collection | DOAJ |
| description | Smoking is an important factor in the pathogenesis of chronic obstructive pulmonary disease (COPD), which is commonly characterised by cellular senescence and inflammation. Recently, miR-200b has emerged as an important target to cure lung disease; however, the function of miR-200b in reducing cellular senescence and inflammatory responses has not been reported. In this study, we found that miR-200b was downregulated in the lungs of COPD model mice, and its expression is correlated with cellular senescence and inflammatory responses. We hypothesised that miR-200b may be a potential novel therapy for treating COPD. We performed senescence-Associated-β-galactosidase (SA-β-GAL) staining, western blot, qRT-PCR and ELISA; our data suggested that miR-200b is an anti-aging factor in the lungs that is involved in inflammatory responses. We also confirmed that ZEB2 (Zinc finger E-box binding homeobox 2) is a target gene of miR-200b using luciferase reporter assay. In addition, we verified the function of ZEB2 in cellular senescence and inflammatory responses through transfection experiments. Moreover, we found that the protective effects of miR-200b are inhibited when cells overexpress the ZEB2 protein. In conclusion, our results suggest that miR-200b may attenuate cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema. |
| format | Article |
| id | doaj-art-c21cfc20ef334cb2a9e65affe2d8b44f |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-c21cfc20ef334cb2a9e65affe2d8b44f2025-08-20T04:02:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148165666310.1080/21691401.2020.1725029miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysemaZhiming Shen0Wenting Xuan1Huanhuan Wang2Fei Sun3Chengxin Zhang4Qian Gong5Shenglin Ge6Department of Cardiovascular Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Anesthesiology, Drum Tower Hospital, Medical College of Nanjing University, Nanjing, ChinaDepartment of Radiation Oncology, First Hospital of Jilin University, Changchun, ChinaDepartment of Cardiovascular Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Cardiovascular Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Cardiac Intensive Care Unit, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Cardiovascular Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaSmoking is an important factor in the pathogenesis of chronic obstructive pulmonary disease (COPD), which is commonly characterised by cellular senescence and inflammation. Recently, miR-200b has emerged as an important target to cure lung disease; however, the function of miR-200b in reducing cellular senescence and inflammatory responses has not been reported. In this study, we found that miR-200b was downregulated in the lungs of COPD model mice, and its expression is correlated with cellular senescence and inflammatory responses. We hypothesised that miR-200b may be a potential novel therapy for treating COPD. We performed senescence-Associated-β-galactosidase (SA-β-GAL) staining, western blot, qRT-PCR and ELISA; our data suggested that miR-200b is an anti-aging factor in the lungs that is involved in inflammatory responses. We also confirmed that ZEB2 (Zinc finger E-box binding homeobox 2) is a target gene of miR-200b using luciferase reporter assay. In addition, we verified the function of ZEB2 in cellular senescence and inflammatory responses through transfection experiments. Moreover, we found that the protective effects of miR-200b are inhibited when cells overexpress the ZEB2 protein. In conclusion, our results suggest that miR-200b may attenuate cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema.https://www.tandfonline.com/doi/10.1080/21691401.2020.1725029miR-200bZEB2pulmonary emphysemacellular senescenceinflammation |
| spellingShingle | Zhiming Shen Wenting Xuan Huanhuan Wang Fei Sun Chengxin Zhang Qian Gong Shenglin Ge miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema Artificial Cells, Nanomedicine, and Biotechnology miR-200b ZEB2 pulmonary emphysema cellular senescence inflammation |
| title | miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema |
| title_full | miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema |
| title_fullStr | miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema |
| title_full_unstemmed | miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema |
| title_short | miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema |
| title_sort | mir 200b regulates cellular senescence and inflammatory responses by targeting zeb2 in pulmonary emphysema |
| topic | miR-200b ZEB2 pulmonary emphysema cellular senescence inflammation |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1725029 |
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