Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage
<b>Aim:</b> The purpose of this study was to investigate the effect of different rate of gallic acid on ram semen liquid storage in non-breeding season.<p> <b>Materials and Methods: </b>In this study, four Merino rams (2-3 ages) were used and belonging to the special sh...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1247 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832543873165950976 |
|---|---|
| author | Şükrü Güngör Muhammed Enes İnanç Ayhan Ata |
| author_facet | Şükrü Güngör Muhammed Enes İnanç Ayhan Ata |
| author_sort | Şükrü Güngör |
| collection | DOAJ |
| description | <b>Aim:</b> The purpose of this study was to investigate the effect of different
rate of gallic acid on ram semen liquid storage in non-breeding
season.<p>
<b>Materials and Methods: </b>In this study, four Merino rams (2-3 ages)
were used and belonging to the special sheep farm in Callica, Burdur/
Turkey. Ejaculates were collected from the rams using a electroejaculator,
the ejaculates containing spermatozoa with >80% motility and
concentrations higher than 1.5x10⁹ spermatozoa/ml were mixed and
used in the study. The mixed ejaculates were divided into four equal
aliquots and samples were extended with tris containing 0 mM (control),
2 mM, 5 mM, 10 mM gallic acid and they were stored at 4°C. After
the groups were created, sperm motility (%), morphologic integrity
(%), membrane integrity subjective (Hipo Osmotic Swelling Test, HOS
test, %) were examined 24 hour intervals until 96 hour.<p>
<b>Results:</b> The highest motility at 48. hour was seen gallic acid 2 mM
(72.7±5.3%) and 5 mM (69.4±4.8%) groups compared the control
(51.0±2.7%) group (P<0.05). At the end of the storage time to (96.
hour) gallic acid 2mM and gallic acid 5 mM was protected to sperm
motilities compared the control group (P<0.05). Also, gallic acid 10
mM were decreased to the membrane integrity compared to the control
group (P < 0.001). There were no significant differences among
groups on abnormal spermatozoa rate in every storage period (P >
0.05) but, there was a statistically significant increase in abnormal
spermatozoa rate at the depending on storage time (P < 0.001).<p>
<b>Conclusion:</b> In conclusion, gallic acid 2 mM and gallic acid 5 mM should
be supplement to ram semen extender at short term storage period
on non-breeding season for improvement of sperm motility. |
| format | Article |
| id | doaj-art-c21c69500ad74aa9914885361155fc7c |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-c21c69500ad74aa9914885361155fc7c2025-02-03T11:25:39ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-195335287921247Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storageŞükrü GüngörMuhammed Enes İnançAyhan Ata<b>Aim:</b> The purpose of this study was to investigate the effect of different rate of gallic acid on ram semen liquid storage in non-breeding season.<p> <b>Materials and Methods: </b>In this study, four Merino rams (2-3 ages) were used and belonging to the special sheep farm in Callica, Burdur/ Turkey. Ejaculates were collected from the rams using a electroejaculator, the ejaculates containing spermatozoa with >80% motility and concentrations higher than 1.5x10⁹ spermatozoa/ml were mixed and used in the study. The mixed ejaculates were divided into four equal aliquots and samples were extended with tris containing 0 mM (control), 2 mM, 5 mM, 10 mM gallic acid and they were stored at 4°C. After the groups were created, sperm motility (%), morphologic integrity (%), membrane integrity subjective (Hipo Osmotic Swelling Test, HOS test, %) were examined 24 hour intervals until 96 hour.<p> <b>Results:</b> The highest motility at 48. hour was seen gallic acid 2 mM (72.7±5.3%) and 5 mM (69.4±4.8%) groups compared the control (51.0±2.7%) group (P<0.05). At the end of the storage time to (96. hour) gallic acid 2mM and gallic acid 5 mM was protected to sperm motilities compared the control group (P<0.05). Also, gallic acid 10 mM were decreased to the membrane integrity compared to the control group (P < 0.001). There were no significant differences among groups on abnormal spermatozoa rate in every storage period (P > 0.05) but, there was a statistically significant increase in abnormal spermatozoa rate at the depending on storage time (P < 0.001).<p> <b>Conclusion:</b> In conclusion, gallic acid 2 mM and gallic acid 5 mM should be supplement to ram semen extender at short term storage period on non-breeding season for improvement of sperm motility.http://eurasianjvetsci.org/pdf.php3?id=1247gallic acidram semenmotilitymembrane integrity |
| spellingShingle | Şükrü Güngör Muhammed Enes İnanç Ayhan Ata Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage Eurasian Journal of Veterinary Sciences gallic acid ram semen motility membrane integrity |
| title | Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage |
| title_full | Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage |
| title_fullStr | Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage |
| title_full_unstemmed | Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage |
| title_short | Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage |
| title_sort | effect of gallic acid on ram semen spermatological parameters at 4 8304 c storage |
| topic | gallic acid ram semen motility membrane integrity |
| url | http://eurasianjvetsci.org/pdf.php3?id=1247 |
| work_keys_str_mv | AT sukrugungor effectofgallicacidonramsemenspermatologicalparametersat48304cstorage AT muhammedenesinanc effectofgallicacidonramsemenspermatologicalparametersat48304cstorage AT ayhanata effectofgallicacidonramsemenspermatologicalparametersat48304cstorage |