Effect of gallic acid on ram semen spermatological parameters at +4 ⁰c storage
<b>Aim:</b> The purpose of this study was to investigate the effect of different rate of gallic acid on ram semen liquid storage in non-breeding season.<p> <b>Materials and Methods: </b>In this study, four Merino rams (2-3 ages) were used and belonging to the special sh...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1247 |
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| Summary: | <b>Aim:</b> The purpose of this study was to investigate the effect of different
rate of gallic acid on ram semen liquid storage in non-breeding
season.<p>
<b>Materials and Methods: </b>In this study, four Merino rams (2-3 ages)
were used and belonging to the special sheep farm in Callica, Burdur/
Turkey. Ejaculates were collected from the rams using a electroejaculator,
the ejaculates containing spermatozoa with >80% motility and
concentrations higher than 1.5x10⁹ spermatozoa/ml were mixed and
used in the study. The mixed ejaculates were divided into four equal
aliquots and samples were extended with tris containing 0 mM (control),
2 mM, 5 mM, 10 mM gallic acid and they were stored at 4°C. After
the groups were created, sperm motility (%), morphologic integrity
(%), membrane integrity subjective (Hipo Osmotic Swelling Test, HOS
test, %) were examined 24 hour intervals until 96 hour.<p>
<b>Results:</b> The highest motility at 48. hour was seen gallic acid 2 mM
(72.7±5.3%) and 5 mM (69.4±4.8%) groups compared the control
(51.0±2.7%) group (P<0.05). At the end of the storage time to (96.
hour) gallic acid 2mM and gallic acid 5 mM was protected to sperm
motilities compared the control group (P<0.05). Also, gallic acid 10
mM were decreased to the membrane integrity compared to the control
group (P < 0.001). There were no significant differences among
groups on abnormal spermatozoa rate in every storage period (P >
0.05) but, there was a statistically significant increase in abnormal
spermatozoa rate at the depending on storage time (P < 0.001).<p>
<b>Conclusion:</b> In conclusion, gallic acid 2 mM and gallic acid 5 mM should
be supplement to ram semen extender at short term storage period
on non-breeding season for improvement of sperm motility. |
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| ISSN: | 1309-6958 2146-1953 |