Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L.
Heavy metal pollution is a significant environmental problem with negative potential impacts on agriculture and human health. In this study, calluses were obtained by using in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from these calluses. The effect o...
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| Format: | Article |
| Language: | English |
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Hasan Eleroğlu
2017-12-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1497 |
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| author | Sinan Aydın Cemil İşlek Bengü Türkyılmaz Ünal |
| author_facet | Sinan Aydın Cemil İşlek Bengü Türkyılmaz Ünal |
| author_sort | Sinan Aydın |
| collection | DOAJ |
| description | Heavy metal pollution is a significant environmental problem with negative potential impacts on agriculture and human health. In this study, calluses were obtained by using in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from these calluses. The effect of silver nitrate (AgNO3) solution added in different concentrations and times to cell suspension cultures of pepper on total protein and total phenolic compound amounts, superoxide dismutase and peroxidase enzyme activities were analysed. Total protein and total phenolic amounts, superoxide dismutase and peroxidase enzyme activities were detected by spectrophotometric methods. When the effects of silver metal on pepper plant were examined, it was determined that silver metal reduced the total protein and phenolic content in the pepper plant cells and especially at higher concentration, in the first 24 hour period, antioxidant enzyme activities increased. |
| format | Article |
| id | doaj-art-c20c16e8c9b94a1d8a6fedb26e8d0fe3 |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-c20c16e8c9b94a1d8a6fedb26e8d0fe32025-08-20T02:02:33ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2017-12-015121544154910.24925/turjaf.v5i12.1544-1549.1497683Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L.Sinan Aydın0Cemil İşlek1Bengü Türkyılmaz Ünal2Niğde Ömer Halisdemir Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, 51240 NiğdeNiğde Ömer Halisdemir Üniversitesi, Fen Edebiyat Fakültesi, Biyoteknoloji Bölümü, 51240 NiğdeNiğde Ömer Halisdemir Üniversitesi, Fen Edebiyat Fakültesi, Biyoteknoloji Bölümü, 51240 NiğdeHeavy metal pollution is a significant environmental problem with negative potential impacts on agriculture and human health. In this study, calluses were obtained by using in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from these calluses. The effect of silver nitrate (AgNO3) solution added in different concentrations and times to cell suspension cultures of pepper on total protein and total phenolic compound amounts, superoxide dismutase and peroxidase enzyme activities were analysed. Total protein and total phenolic amounts, superoxide dismutase and peroxidase enzyme activities were detected by spectrophotometric methods. When the effects of silver metal on pepper plant were examined, it was determined that silver metal reduced the total protein and phenolic content in the pepper plant cells and especially at higher concentration, in the first 24 hour period, antioxidant enzyme activities increased.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1497AgNO3Heavy metalPepperPeroxidaseSuperoxide dismutase |
| spellingShingle | Sinan Aydın Cemil İşlek Bengü Türkyılmaz Ünal Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L. Turkish Journal of Agriculture: Food Science and Technology AgNO3 Heavy metal Pepper Peroxidase Superoxide dismutase |
| title | Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L. |
| title_full | Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L. |
| title_fullStr | Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L. |
| title_full_unstemmed | Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L. |
| title_short | Effect of Silver Nitrate Solvent on Total Protein, Total Phenolic and Some Antioxidant Enzyme Activities in Cell Suspension Culture of Capsicum annuum L. |
| title_sort | effect of silver nitrate solvent on total protein total phenolic and some antioxidant enzyme activities in cell suspension culture of capsicum annuum l |
| topic | AgNO3 Heavy metal Pepper Peroxidase Superoxide dismutase |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1497 |
| work_keys_str_mv | AT sinanaydın effectofsilvernitratesolventontotalproteintotalphenolicandsomeantioxidantenzymeactivitiesincellsuspensioncultureofcapsicumannuuml AT cemilislek effectofsilvernitratesolventontotalproteintotalphenolicandsomeantioxidantenzymeactivitiesincellsuspensioncultureofcapsicumannuuml AT benguturkyılmazunal effectofsilvernitratesolventontotalproteintotalphenolicandsomeantioxidantenzymeactivitiesincellsuspensioncultureofcapsicumannuuml |