Differential regulation of mTOR activity in retinal ganglion cells underlies their distinct susceptibility to ischemia/reperfusion

Abstract Retinal ischemia/reperfusion (I/R) injury drives progressive retinal ganglion cell (RGC) loss, yet mechanisms underlying neuronal type-specific vulnerability remain unclear. Using a mouse model of bilateral common carotid artery ligation, we observe decreased vessel density in the inner ret...

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Bibliographic Details
Main Authors: Meizhen Zhu, Yuqing Wu, Hang Gao, Fujian Qi, Xiaoyu Zhang, Yanli Ran
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08314-2
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Summary:Abstract Retinal ischemia/reperfusion (I/R) injury drives progressive retinal ganglion cell (RGC) loss, yet mechanisms underlying neuronal type-specific vulnerability remain unclear. Using a mouse model of bilateral common carotid artery ligation, we observe decreased vessel density in the inner retina following I/R. Here, the total RGCs exhibit much more cell loss than the intrinsic photosensitive RGCs (ipRGCs). This disparity is parallel with their different mTOR activity: for ipRGCs, the mTOR activity is much higher than the total RGCs both before and after I/R. Pharmacological experiments reveal that, mTOR activation in total RGCs and high mTOR activity maintenance in ipRGCs promote rapamycin to protect the total RGCs and ipRGCs against I/R injury, respectively. Notably, the protective effects of rapamycin on total RGCs and ipRGCs manifest under different light conditions. Our findings bridge the type-specific mTOR regulation in RGCs and their distinct susceptibility to I/R injury, which offers new insights into further targeted neuroprotection.
ISSN:2399-3642