Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma
N6-methyladenosine (m6A) is the most common epigenetic modification existing in eukaryocyte transcripts. However, genes related to m6A modification in oral squamous cell carcinoma (OSCC) are still unclear. Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A l...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/7277583 |
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| author | Xi Wang Jie Wu Leyu Zhang Wei Zhao Jiayin Deng |
| author_facet | Xi Wang Jie Wu Leyu Zhang Wei Zhao Jiayin Deng |
| author_sort | Xi Wang |
| collection | DOAJ |
| description | N6-methyladenosine (m6A) is the most common epigenetic modification existing in eukaryocyte transcripts. However, genes related to m6A modification in oral squamous cell carcinoma (OSCC) are still unclear. Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A landscape in OSCC and corresponding controls. The m6A peaks are always distributed in the junction of the 3′-untranslated regions (3′-UTRs) and the coding sequences (CDS) of mRNAs, as well as the entire genome of long noncoding RNA (lncRNA). Furthermore, enrichment analysis showed that differentially methylated genes were significantly enriched in NF-kappa B signaling pathway, Hedgehog signaling pathway, etc. In summary, our findings reveal the landscape of m6A modification on mRNAs and lncRNAs in OSCC, which may provide key clues for the precision-targeted therapy of OSCC. |
| format | Article |
| id | doaj-art-c204c8df949f4dc591248b61602fa6fc |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-c204c8df949f4dc591248b61602fa6fc2025-02-03T01:32:27ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/7277583Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell CarcinomaXi Wang0Jie Wu1Leyu Zhang2Wei Zhao3Jiayin Deng4The School and Hospital of StomatologyThe School and Hospital of StomatologyThe School and Hospital of StomatologyThe School and Hospital of StomatologyThe School and Hospital of StomatologyN6-methyladenosine (m6A) is the most common epigenetic modification existing in eukaryocyte transcripts. However, genes related to m6A modification in oral squamous cell carcinoma (OSCC) are still unclear. Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A landscape in OSCC and corresponding controls. The m6A peaks are always distributed in the junction of the 3′-untranslated regions (3′-UTRs) and the coding sequences (CDS) of mRNAs, as well as the entire genome of long noncoding RNA (lncRNA). Furthermore, enrichment analysis showed that differentially methylated genes were significantly enriched in NF-kappa B signaling pathway, Hedgehog signaling pathway, etc. In summary, our findings reveal the landscape of m6A modification on mRNAs and lncRNAs in OSCC, which may provide key clues for the precision-targeted therapy of OSCC.http://dx.doi.org/10.1155/2022/7277583 |
| spellingShingle | Xi Wang Jie Wu Leyu Zhang Wei Zhao Jiayin Deng Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma Journal of Immunology Research |
| title | Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma |
| title_full | Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma |
| title_fullStr | Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma |
| title_short | Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma |
| title_sort | methylated rna immunoprecipitation sequencing reveals the m6a landscape in oral squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2022/7277583 |
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