Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits
Sustainable agro-waste revaluation is critical to enhance the profitability and environmental footprint of the olive oil industry. Herein, the valorization of olive leaf pruning waste from five cultivars (‘Caiazzana’, ‘Carolea’, ‘Itrana’, ‘Leccino’, and ‘Frantoio’) employed green extraction methods...
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2025-01-01
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| author | Hamid Mushtaq Simona Piccolella Jose A. Mendiola Lidia Montero Elena Ibáñez Severina Pacifico |
| author_facet | Hamid Mushtaq Simona Piccolella Jose A. Mendiola Lidia Montero Elena Ibáñez Severina Pacifico |
| author_sort | Hamid Mushtaq |
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| description | Sustainable agro-waste revaluation is critical to enhance the profitability and environmental footprint of the olive oil industry. Herein, the valorization of olive leaf pruning waste from five cultivars (‘Caiazzana’, ‘Carolea’, ‘Itrana’, ‘Leccino’, and ‘Frantoio’) employed green extraction methods to recover compounds with potential health benefits. Sequential ultrasound-assisted maceration (UAM) in <i>n</i>-hexane and ethanol was compared with a compressed fluid extraction strategy consisting of supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) for their efficiency in recovering distinct classes of bioactives. Chemical profiling by UHPLC-HR-MS/MS (ultra-high-performance liquid chromatography high-resolution tandem mass spectrometry) and GC-MS (gas chromatography mass spectrometry) showed that UAM-EtOH effectively extracted polyphenols (especially luteolin derivatives) and triterpenes (notably maslinic acid), while PLE yielded the highest amount of secoiridoids (e.g., secologanoside). PLE extracts showed better antiradical activities, putatively due to a higher content of flavonoids, secoiridoids, and HCA derivatives than UAM-EtOH ones, as these latter also contained 20–40% (cultivar-dependent) of triterpenes. SFE extracts with a higher concentration of fatty acids and triterpenes showed moderate antioxidant activities but very high AChE inhibition. This study highlights the importance of selecting appropriate extraction methodologies based on the target bioactive compounds and underscores the potential of olive leaf extracts for sustainable bio-products. |
| format | Article |
| id | doaj-art-c1ed5d3cd25f430fb30c0426cec39ee7 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-c1ed5d3cd25f430fb30c0426cec39ee72025-01-24T13:33:11ZengMDPI AGFoods2304-81582025-01-0114229710.3390/foods14020297Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health BenefitsHamid Mushtaq0Simona Piccolella1Jose A. Mendiola2Lidia Montero3Elena Ibáñez4Severina Pacifico5Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyFoodomics Laboratory, Institute of Food Science Research CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, SpainFoodomics Laboratory, Institute of Food Science Research CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, SpainFoodomics Laboratory, Institute of Food Science Research CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, SpainDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalySustainable agro-waste revaluation is critical to enhance the profitability and environmental footprint of the olive oil industry. Herein, the valorization of olive leaf pruning waste from five cultivars (‘Caiazzana’, ‘Carolea’, ‘Itrana’, ‘Leccino’, and ‘Frantoio’) employed green extraction methods to recover compounds with potential health benefits. Sequential ultrasound-assisted maceration (UAM) in <i>n</i>-hexane and ethanol was compared with a compressed fluid extraction strategy consisting of supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) for their efficiency in recovering distinct classes of bioactives. Chemical profiling by UHPLC-HR-MS/MS (ultra-high-performance liquid chromatography high-resolution tandem mass spectrometry) and GC-MS (gas chromatography mass spectrometry) showed that UAM-EtOH effectively extracted polyphenols (especially luteolin derivatives) and triterpenes (notably maslinic acid), while PLE yielded the highest amount of secoiridoids (e.g., secologanoside). PLE extracts showed better antiradical activities, putatively due to a higher content of flavonoids, secoiridoids, and HCA derivatives than UAM-EtOH ones, as these latter also contained 20–40% (cultivar-dependent) of triterpenes. SFE extracts with a higher concentration of fatty acids and triterpenes showed moderate antioxidant activities but very high AChE inhibition. This study highlights the importance of selecting appropriate extraction methodologies based on the target bioactive compounds and underscores the potential of olive leaf extracts for sustainable bio-products.https://www.mdpi.com/2304-8158/14/2/297<i>Olea europaea</i> L.waste valorizationUHPLC-HR MS/MS analysisUAMSFEPLE |
| spellingShingle | Hamid Mushtaq Simona Piccolella Jose A. Mendiola Lidia Montero Elena Ibáñez Severina Pacifico Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits Foods <i>Olea europaea</i> L. waste valorization UHPLC-HR MS/MS analysis UAM SFE PLE |
| title | Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits |
| title_full | Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits |
| title_fullStr | Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits |
| title_full_unstemmed | Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits |
| title_short | Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits |
| title_sort | recovery of bioactive constituents from olive leaf pruning waste of five different cultivars a comparison of green extraction techniques to maximize health benefits |
| topic | <i>Olea europaea</i> L. waste valorization UHPLC-HR MS/MS analysis UAM SFE PLE |
| url | https://www.mdpi.com/2304-8158/14/2/297 |
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