Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function
Renal transplant candidates present immune dysregulation, caused by chronic uremia. The aim of the study was to investigate whether pretransplant peripheral blood gene expression of immune factors affects clinical outcome of renal allograft recipients. Methods. In a prospective study, we analyzed pr...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/8970291 |
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| author | Dorota Kamińska Katarzyna Kościelska-Kasprzak Paweł Chudoba Oktawia Mazanowska Mirosław Banasik Marcelina Żabinska Maria Boratyńska Agnieszka Lepiesza Agnieszka Gomółkiewicz Piotr Dzięgiel Marian Klinger |
| author_facet | Dorota Kamińska Katarzyna Kościelska-Kasprzak Paweł Chudoba Oktawia Mazanowska Mirosław Banasik Marcelina Żabinska Maria Boratyńska Agnieszka Lepiesza Agnieszka Gomółkiewicz Piotr Dzięgiel Marian Klinger |
| author_sort | Dorota Kamińska |
| collection | DOAJ |
| description | Renal transplant candidates present immune dysregulation, caused by chronic uremia. The aim of the study was to investigate whether pretransplant peripheral blood gene expression of immune factors affects clinical outcome of renal allograft recipients. Methods. In a prospective study, we analyzed pretransplant peripheral blood gene expression in87 renal transplant candidates with real-time PCR on custom-designed low density arrays (TaqMan). Results. Immediate posttransplant graft function (14-day GFR) was influenced negatively by TGFB1 (P=0.039) and positively by IL-2 gene expression (P=0.040). Pretransplant blood mRNA expression of apoptosis-related genes (CASP3, FAS, and IL-18) and Th1-derived cytokine gene IFNG correlated positively with short- (6-month GFR CASP3: P=0.027, FAS: P=0.021, and IFNG: P=0.029) and long-term graft function (24-month GFR CASP3: P=0.003, FAS: P=0.033, IL-18: P=0.044, and IFNG: P=0.04). Conclusion. Lowered pretransplant Th1-derived cytokine and apoptosis-related gene expressions were a hallmark of subsequent worse kidney function but not of acute rejection rate. The pretransplant IFNG and CASP3 and FAS and IL-18 genes’ expression in the recipients’ peripheral blood is the possible candidate for novel biomarker of short- and long-term allograft function. |
| format | Article |
| id | doaj-art-c1e30925d4a04093a2c26586f3906ea0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c1e30925d4a04093a2c26586f3906ea02025-02-03T01:21:21ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/89702918970291Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft FunctionDorota Kamińska0Katarzyna Kościelska-Kasprzak1Paweł Chudoba2Oktawia Mazanowska3Mirosław Banasik4Marcelina Żabinska5Maria Boratyńska6Agnieszka Lepiesza7Agnieszka Gomółkiewicz8Piotr Dzięgiel9Marian Klinger10Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Vascular, General and Transplant Surgery, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Vascular, General and Transplant Surgery, Wroclaw Medical University, 52-129 Wrocław, PolandDepartment of Histology and Embryology, Wroclaw Medical University, 50-368 Wrocław, PolandDepartment of Histology and Embryology, Wroclaw Medical University, 50-368 Wrocław, PolandDepartment of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, PolandRenal transplant candidates present immune dysregulation, caused by chronic uremia. The aim of the study was to investigate whether pretransplant peripheral blood gene expression of immune factors affects clinical outcome of renal allograft recipients. Methods. In a prospective study, we analyzed pretransplant peripheral blood gene expression in87 renal transplant candidates with real-time PCR on custom-designed low density arrays (TaqMan). Results. Immediate posttransplant graft function (14-day GFR) was influenced negatively by TGFB1 (P=0.039) and positively by IL-2 gene expression (P=0.040). Pretransplant blood mRNA expression of apoptosis-related genes (CASP3, FAS, and IL-18) and Th1-derived cytokine gene IFNG correlated positively with short- (6-month GFR CASP3: P=0.027, FAS: P=0.021, and IFNG: P=0.029) and long-term graft function (24-month GFR CASP3: P=0.003, FAS: P=0.033, IL-18: P=0.044, and IFNG: P=0.04). Conclusion. Lowered pretransplant Th1-derived cytokine and apoptosis-related gene expressions were a hallmark of subsequent worse kidney function but not of acute rejection rate. The pretransplant IFNG and CASP3 and FAS and IL-18 genes’ expression in the recipients’ peripheral blood is the possible candidate for novel biomarker of short- and long-term allograft function.http://dx.doi.org/10.1155/2016/8970291 |
| spellingShingle | Dorota Kamińska Katarzyna Kościelska-Kasprzak Paweł Chudoba Oktawia Mazanowska Mirosław Banasik Marcelina Żabinska Maria Boratyńska Agnieszka Lepiesza Agnieszka Gomółkiewicz Piotr Dzięgiel Marian Klinger Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function Mediators of Inflammation |
| title | Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function |
| title_full | Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function |
| title_fullStr | Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function |
| title_full_unstemmed | Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function |
| title_short | Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function |
| title_sort | pretransplant immune and apoptosis related gene expression is associated with kidney allograft function |
| url | http://dx.doi.org/10.1155/2016/8970291 |
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