The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus
Vibrio parahaemolyticus is a zoonotic pathogen that poses a serious threat to shrimp aquaculture and public health worldwide. Copper (Cu) serves as a catalytic cofactor and structural element in bacteria but is toxic in excess. Herein, we report that the CueR-regulated transporters CopA and CusFABC...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2545557 |
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| author | Chengkun Zheng Yimeng Zhai Mengxian Wang Zhengzhong Xu Yunzeng Zhang Xiaohui Zhou Xiang Chen Xinan Jiao |
| author_facet | Chengkun Zheng Yimeng Zhai Mengxian Wang Zhengzhong Xu Yunzeng Zhang Xiaohui Zhou Xiang Chen Xinan Jiao |
| author_sort | Chengkun Zheng |
| collection | DOAJ |
| description | Vibrio parahaemolyticus is a zoonotic pathogen that poses a serious threat to shrimp aquaculture and public health worldwide. Copper (Cu) serves as a catalytic cofactor and structural element in bacteria but is toxic in excess. Herein, we report that the CueR-regulated transporters CopA and CusFABC coordinate Cu detoxification in V. parahaemolyticus. Through RNA sequencing, we show that most genes in the CueR and Fur regulons are significantly upregulated during V. parahaemolyticus response to external Cu. Quantitative real-time PCR (qRT-PCR) showed that copA and cusA are strongly induced by Cu. Growth curve analyses and spot dilution assay revealed that CopA and CusFABC protect V. parahaemolyticus against Cu-induced bactericidal effects. Inductively coupled plasma-mass spectrometry revealed that CopA and CusFABC are involved in maintaining Cu and Fe homoeostasis. Additionally, CopA promotes V. parahaemolyticus resistance to pyrithione. In a competitive-infection assay, the copA and cusFABC double mutant, but not the single mutants, was significantly attenuated in colonization of the zebrafish intestine. Lastly, qRT-PCR, RNA sequencing, β-galactosidase activity, and AIDmut-Seq analyses revealed that CueR can bind to the promoters of copA and cusFABC, and positively regulate their expression. Collectively, V. parahaemolyticus utilizes the CueR-regulated transporters CopA and CusFABC to coordinate Cu detoxification and colonization of the zebrafish intestine. |
| format | Article |
| id | doaj-art-c1e172ce218e43fca63473e9c88bf44c |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-c1e172ce218e43fca63473e9c88bf44c2025-08-20T04:02:28ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2545557The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticusChengkun Zheng0Yimeng Zhai1Mengxian Wang2Zhengzhong Xu3Yunzeng Zhang4Xiaohui Zhou5Xiang Chen6Xinan Jiao7Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaSchool of Public Health and Emergency Management, Southern University of Science and Technology, Shenzhen, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, the Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou, ChinaVibrio parahaemolyticus is a zoonotic pathogen that poses a serious threat to shrimp aquaculture and public health worldwide. Copper (Cu) serves as a catalytic cofactor and structural element in bacteria but is toxic in excess. Herein, we report that the CueR-regulated transporters CopA and CusFABC coordinate Cu detoxification in V. parahaemolyticus. Through RNA sequencing, we show that most genes in the CueR and Fur regulons are significantly upregulated during V. parahaemolyticus response to external Cu. Quantitative real-time PCR (qRT-PCR) showed that copA and cusA are strongly induced by Cu. Growth curve analyses and spot dilution assay revealed that CopA and CusFABC protect V. parahaemolyticus against Cu-induced bactericidal effects. Inductively coupled plasma-mass spectrometry revealed that CopA and CusFABC are involved in maintaining Cu and Fe homoeostasis. Additionally, CopA promotes V. parahaemolyticus resistance to pyrithione. In a competitive-infection assay, the copA and cusFABC double mutant, but not the single mutants, was significantly attenuated in colonization of the zebrafish intestine. Lastly, qRT-PCR, RNA sequencing, β-galactosidase activity, and AIDmut-Seq analyses revealed that CueR can bind to the promoters of copA and cusFABC, and positively regulate their expression. Collectively, V. parahaemolyticus utilizes the CueR-regulated transporters CopA and CusFABC to coordinate Cu detoxification and colonization of the zebrafish intestine.https://www.tandfonline.com/doi/10.1080/21505594.2025.2545557Vibrio parahaemolyticuscopper detoxificationCueRCopACusFABC |
| spellingShingle | Chengkun Zheng Yimeng Zhai Mengxian Wang Zhengzhong Xu Yunzeng Zhang Xiaohui Zhou Xiang Chen Xinan Jiao The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus Virulence Vibrio parahaemolyticus copper detoxification CueR CopA CusFABC |
| title | The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus |
| title_full | The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus |
| title_fullStr | The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus |
| title_full_unstemmed | The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus |
| title_short | The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification in Vibrio parahaemolyticus |
| title_sort | cuer regulated transporters copa and cusfabc coordinate copper detoxification in vibrio parahaemolyticus |
| topic | Vibrio parahaemolyticus copper detoxification CueR CopA CusFABC |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2545557 |
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