Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat
As one of the most important cereals, wheat (Triticum aestivum) has high nutritional value and is widely cultivated in China. However, wheat can cause severe allergic reactions, and a growing number of people are developing allergies to Chinese wheat. Low molecular weight glutenin (LMW-GS), an impor...
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| Language: | English |
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001926 |
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| author | Yanbo Wang Yihang Tong Jinru Zhou Dong Yang Linglin Fu |
| author_facet | Yanbo Wang Yihang Tong Jinru Zhou Dong Yang Linglin Fu |
| author_sort | Yanbo Wang |
| collection | DOAJ |
| description | As one of the most important cereals, wheat (Triticum aestivum) has high nutritional value and is widely cultivated in China. However, wheat can cause severe allergic reactions, and a growing number of people are developing allergies to Chinese wheat. Low molecular weight glutenin (LMW-GS), an important allergen in susceptible populations, is responsible for celiac disease and wheat contacts dermatitis. In this study, LMW-GS was highly purified from Chinese wheat (Xiaoyan 6) and further identified and characterized. In addition, 8 peptides were predicted efficiently by 5 immunological tools, among which five peptides showed significant immunoglobulin E (IgE) binding abilities. Two specific epitopes were found to be in the non-conserved region of the amino acid sequence of LMW-GS, which was speculated to be the specific epitope of Chinese wheat. This systematic research of LMW-GS may provide new insights into the prevention of wheat allergy and development of hypoallergenic wheat products. |
| format | Article |
| id | doaj-art-c1df39c2465041f3883cbe1f2d4c4f72 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c1df39c2465041f3883cbe1f2d4c4f722025-02-03T06:53:35ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123720727Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheatYanbo Wang0Yihang Tong1Jinru Zhou2Dong Yang3Linglin Fu4Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; Corresponding author at: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xue Zheng Street, Hangzhou 310018, China,As one of the most important cereals, wheat (Triticum aestivum) has high nutritional value and is widely cultivated in China. However, wheat can cause severe allergic reactions, and a growing number of people are developing allergies to Chinese wheat. Low molecular weight glutenin (LMW-GS), an important allergen in susceptible populations, is responsible for celiac disease and wheat contacts dermatitis. In this study, LMW-GS was highly purified from Chinese wheat (Xiaoyan 6) and further identified and characterized. In addition, 8 peptides were predicted efficiently by 5 immunological tools, among which five peptides showed significant immunoglobulin E (IgE) binding abilities. Two specific epitopes were found to be in the non-conserved region of the amino acid sequence of LMW-GS, which was speculated to be the specific epitope of Chinese wheat. This systematic research of LMW-GS may provide new insights into the prevention of wheat allergy and development of hypoallergenic wheat products.http://www.sciencedirect.com/science/article/pii/S2213453022001926Triticum aestivumAllergenLow molecular weight glutenin (LMW-GS)Immunological tools |
| spellingShingle | Yanbo Wang Yihang Tong Jinru Zhou Dong Yang Linglin Fu Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat Food Science and Human Wellness Triticum aestivum Allergen Low molecular weight glutenin (LMW-GS) Immunological tools |
| title | Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat |
| title_full | Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat |
| title_fullStr | Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat |
| title_full_unstemmed | Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat |
| title_short | Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat |
| title_sort | purification and immunoglobulin e epitopes identification of low molecular weight glutenin an allergen in chinese wheat |
| topic | Triticum aestivum Allergen Low molecular weight glutenin (LMW-GS) Immunological tools |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001926 |
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