<i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro
<i>Pseudomonas aeruginosa</i> and <i>Mycobacterium abscessus</i> are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential imp...
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2025-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/1/116 |
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| author | Yun Long Zhi Li Menglu Li Peiyi Lu Yujia Deng Pengyao Wu Xue Li Gangjian Qin Jiamin Huang Wenying Gao Guobao Li Tianyuan Jia Liang Yang |
| author_facet | Yun Long Zhi Li Menglu Li Peiyi Lu Yujia Deng Pengyao Wu Xue Li Gangjian Qin Jiamin Huang Wenying Gao Guobao Li Tianyuan Jia Liang Yang |
| author_sort | Yun Long |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> and <i>Mycobacterium abscessus</i> are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential impacts on infections are largely unexplored. In this study, we first demonstrated that <i>P. aeruginosa</i> inhibits the growth of <i>M. abscessus</i> by iron chelating via <i>pqs</i> quorum sensing. Next, through proteomic analysis, we discovered that the PQS molecule significantly changed a large amount of protein expression in <i>M. abscessus</i>, including proteins involved in the type VII secretion system and iron homeostasis. Furthermore, we revealed that PQS significantly enhanced the production of bacterial membrane vesicles (MVs) by <i>M. abscessus</i>. Our study suggests that the <i>P. aeruginosa</i> PQS can serve as an interspecies signaling molecule to communicate with <i>Mycobacterium</i> and affect their physiology and virulence. |
| format | Article |
| id | doaj-art-c1cab9929cb746ce84e310c9d227adce |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-c1cab9929cb746ce84e310c9d227adce2025-01-24T13:42:42ZengMDPI AGMicroorganisms2076-26072025-01-0113111610.3390/microorganisms13010116<i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In VitroYun Long0Zhi Li1Menglu Li2Peiyi Lu3Yujia Deng4Pengyao Wu5Xue Li6Gangjian Qin7Jiamin Huang8Wenying Gao9Guobao Li10Tianyuan Jia11Liang Yang12Shenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaDepartment of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, ChinaDepartment of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, ChinaDepartment of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, ChinaDepartment of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaDepartment of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, ChinaShenzhen Third People’s Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China<i>Pseudomonas aeruginosa</i> and <i>Mycobacterium abscessus</i> are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential impacts on infections are largely unexplored. In this study, we first demonstrated that <i>P. aeruginosa</i> inhibits the growth of <i>M. abscessus</i> by iron chelating via <i>pqs</i> quorum sensing. Next, through proteomic analysis, we discovered that the PQS molecule significantly changed a large amount of protein expression in <i>M. abscessus</i>, including proteins involved in the type VII secretion system and iron homeostasis. Furthermore, we revealed that PQS significantly enhanced the production of bacterial membrane vesicles (MVs) by <i>M. abscessus</i>. Our study suggests that the <i>P. aeruginosa</i> PQS can serve as an interspecies signaling molecule to communicate with <i>Mycobacterium</i> and affect their physiology and virulence.https://www.mdpi.com/2076-2607/13/1/116<i>P. aeruginosa</i><i>M. abscessus</i>PQSmembrane vesiclesthe type VII secretion system |
| spellingShingle | Yun Long Zhi Li Menglu Li Peiyi Lu Yujia Deng Pengyao Wu Xue Li Gangjian Qin Jiamin Huang Wenying Gao Guobao Li Tianyuan Jia Liang Yang <i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro Microorganisms <i>P. aeruginosa</i> <i>M. abscessus</i> PQS membrane vesicles the type VII secretion system |
| title | <i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro |
| title_full | <i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro |
| title_fullStr | <i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro |
| title_full_unstemmed | <i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro |
| title_short | <i>Pseudomonas aeruginosa pqs</i> Quorum Sensing Mediates Interaction with <i>Mycobacterium abscessus</i> In Vitro |
| title_sort | i pseudomonas aeruginosa pqs i quorum sensing mediates interaction with i mycobacterium abscessus i in vitro |
| topic | <i>P. aeruginosa</i> <i>M. abscessus</i> PQS membrane vesicles the type VII secretion system |
| url | https://www.mdpi.com/2076-2607/13/1/116 |
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