Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago
The study was conducted to determine the frequency of isolation of Salmonella, Campylobacter and E. coli O157 in the faecal samples of rats trapped across the regional corporations in Trinidad and to assess their resistance to antimicrobial agents. A total of 204 rats were trapped for the detection...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2011/686923 |
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| author | Comfort Nkogwe Juliah Raletobana Alva Stewart-Johnson Sharianne Suepaul Abiodun Adesiyun |
| author_facet | Comfort Nkogwe Juliah Raletobana Alva Stewart-Johnson Sharianne Suepaul Abiodun Adesiyun |
| author_sort | Comfort Nkogwe |
| collection | DOAJ |
| description | The study was conducted to determine the frequency of isolation of Salmonella, Campylobacter and E. coli O157 in the faecal samples of rats trapped across the regional corporations in Trinidad and to assess their resistance to antimicrobial agents. A total of 204 rats were trapped for the detection of selected bacteria. Standard methods were used to isolate Salmonella, Campylobacter and E. coli O157. Characterization of E. coli was done on sorbitol MacConkey agar to determine non-sorbitol fermentation, blood agar to determine haemolytic and mucoid colonies and by using E. coli O157 antiserum to determine O157 strain. The disc diffusion method was used to determine resistance to nine antimicrobial agents. Of the 204 rats, 4 (2.0%), 7 (3.4%) and 171 (83.8%) were positive for Salmonella spp., Campylobacter spp. and E. coli, respectively. Of the 171 isolates of E. coli tested 0 (0.0%), 25 (14.6%) and 19 (11.1%) were haemolytic, mucoid and non-sorbitol fermenters, respectively. All isolates were negative for the O157 strain. The frequency of resistance to the 9 antimicrobial agents tested was 75% (3 of 4) for Salmonella, 85.7% (6 of 7) of Campylobacter spp. and 36.3% (62 of 171) for E. coli (𝑃<.05; χ2). |
| format | Article |
| id | doaj-art-c1c7285432d54ff3ac17b791b4d0be00 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-c1c7285432d54ff3ac17b791b4d0be002025-02-03T01:32:05ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/686923686923Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and TobagoComfort Nkogwe0Juliah Raletobana1Alva Stewart-Johnson2Sharianne Suepaul3Abiodun Adesiyun4School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoSchool of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoSchool of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoSchool of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoSchool of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoThe study was conducted to determine the frequency of isolation of Salmonella, Campylobacter and E. coli O157 in the faecal samples of rats trapped across the regional corporations in Trinidad and to assess their resistance to antimicrobial agents. A total of 204 rats were trapped for the detection of selected bacteria. Standard methods were used to isolate Salmonella, Campylobacter and E. coli O157. Characterization of E. coli was done on sorbitol MacConkey agar to determine non-sorbitol fermentation, blood agar to determine haemolytic and mucoid colonies and by using E. coli O157 antiserum to determine O157 strain. The disc diffusion method was used to determine resistance to nine antimicrobial agents. Of the 204 rats, 4 (2.0%), 7 (3.4%) and 171 (83.8%) were positive for Salmonella spp., Campylobacter spp. and E. coli, respectively. Of the 171 isolates of E. coli tested 0 (0.0%), 25 (14.6%) and 19 (11.1%) were haemolytic, mucoid and non-sorbitol fermenters, respectively. All isolates were negative for the O157 strain. The frequency of resistance to the 9 antimicrobial agents tested was 75% (3 of 4) for Salmonella, 85.7% (6 of 7) of Campylobacter spp. and 36.3% (62 of 171) for E. coli (𝑃<.05; χ2).http://dx.doi.org/10.4061/2011/686923 |
| spellingShingle | Comfort Nkogwe Juliah Raletobana Alva Stewart-Johnson Sharianne Suepaul Abiodun Adesiyun Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago Veterinary Medicine International |
| title | Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago |
| title_full | Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago |
| title_fullStr | Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago |
| title_full_unstemmed | Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago |
| title_short | Frequency of Detection of Escherichia coli, Salmonella spp., and Campylobacter spp. in the Faeces of Wild Rats (Rattus spp.) in Trinidad and Tobago |
| title_sort | frequency of detection of escherichia coli salmonella spp and campylobacter spp in the faeces of wild rats rattus spp in trinidad and tobago |
| url | http://dx.doi.org/10.4061/2011/686923 |
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