The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury
Purpose. To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI). Methods. OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control (n =...
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| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/5565178 |
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| author | Xiao Zhao Han Han Yinting Song Mei Du Mengyu Liao Xue Dong Xiaohong Wang Ferenc Kuhn Annette Hoskin Heping Xu Hua Yan |
| author_facet | Xiao Zhao Han Han Yinting Song Mei Du Mengyu Liao Xue Dong Xiaohong Wang Ferenc Kuhn Annette Hoskin Heping Xu Hua Yan |
| author_sort | Xiao Zhao |
| collection | DOAJ |
| description | Purpose. To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI). Methods. OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control (n = 5), OGI group (n = 40), and intravitreal Ranibizumab and Conbercept (n = 15 each). Ranibizumab or Conbercept was injected into the vitreous at 0.5 hours, 3 days, or 7 days. Vitreous fluid was collected, and levels of growth factors and cytokines were measured by enzyme-linked immunosorbent assay (ELISA). On day 28 after OGI, B scan examination and histological examination were performed to evaluate intravitreal proliferation and formation of epiretinal fibrosis. Results. Vitreous levels of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-β), and plasminogen activator inhibitor-1 (PAI-1) were significantly increased in rabbit eyes after OGI. Compared to eyes in OGI group, anti-VEGF treatments significantly reduced these growth factors and cytokines. Among the 7 eyes examined from each group for intravitreal proliferative changes, they were found in 7 of 7 (100%) in OGI group and were decreased by Ranibizumab and Conbercept to 5 of 7 (71.4%) and 4 of 7 (57.1%), respectively. Both Ranibizumab and Conbercept inhibited epiretinal scar formation at the wound site, with Conbercept showing the greatest effect (maximal length of scar (L), LOGI = 503 ± 82.44 μm, LRanibizumab = 355 ± 43.66 μm, and LConbercept = 250.33 ± 36.02 μm). Conclusion. Anti-VEGF treatments after OGI significantly attenuated the upregulation of growth factors and cytokines in the vitreous and prevented intravitreal proliferation and epiretinal scar formation and thus may protect against the development of posttraumatic complications such as proliferative vitreoretinopathy (PVR). |
| format | Article |
| id | doaj-art-c1ace0ec17694840b854751d9715449b |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-c1ace0ec17694840b854751d9715449b2025-02-03T05:52:38ZengWileyJournal of Ophthalmology2090-004X2090-00582021-01-01202110.1155/2021/55651785565178The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe InjuryXiao Zhao0Han Han1Yinting Song2Mei Du3Mengyu Liao4Xue Dong5Xiaohong Wang6Ferenc Kuhn7Annette Hoskin8Heping Xu9Hua Yan10Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaLaboratory of Molecular Ophthalmology, Department of Pharmacology and Tianjin Key Laboratory of Inflammation Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaLaboratory of Molecular Ophthalmology, Department of Pharmacology and Tianjin Key Laboratory of Inflammation Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaHelen Keller Foundation for Research and Education, Birmingham, AL, USASave Sight Institute, The University of Sydney, Sydney, NSW, AustraliaCentre for Experimental Medicine, School of Medicine, Dentistry & Biomedical Sciences, Queen’s University Belfast, Belfast, UKDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaPurpose. To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI). Methods. OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control (n = 5), OGI group (n = 40), and intravitreal Ranibizumab and Conbercept (n = 15 each). Ranibizumab or Conbercept was injected into the vitreous at 0.5 hours, 3 days, or 7 days. Vitreous fluid was collected, and levels of growth factors and cytokines were measured by enzyme-linked immunosorbent assay (ELISA). On day 28 after OGI, B scan examination and histological examination were performed to evaluate intravitreal proliferation and formation of epiretinal fibrosis. Results. Vitreous levels of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-β), and plasminogen activator inhibitor-1 (PAI-1) were significantly increased in rabbit eyes after OGI. Compared to eyes in OGI group, anti-VEGF treatments significantly reduced these growth factors and cytokines. Among the 7 eyes examined from each group for intravitreal proliferative changes, they were found in 7 of 7 (100%) in OGI group and were decreased by Ranibizumab and Conbercept to 5 of 7 (71.4%) and 4 of 7 (57.1%), respectively. Both Ranibizumab and Conbercept inhibited epiretinal scar formation at the wound site, with Conbercept showing the greatest effect (maximal length of scar (L), LOGI = 503 ± 82.44 μm, LRanibizumab = 355 ± 43.66 μm, and LConbercept = 250.33 ± 36.02 μm). Conclusion. Anti-VEGF treatments after OGI significantly attenuated the upregulation of growth factors and cytokines in the vitreous and prevented intravitreal proliferation and epiretinal scar formation and thus may protect against the development of posttraumatic complications such as proliferative vitreoretinopathy (PVR).http://dx.doi.org/10.1155/2021/5565178 |
| spellingShingle | Xiao Zhao Han Han Yinting Song Mei Du Mengyu Liao Xue Dong Xiaohong Wang Ferenc Kuhn Annette Hoskin Heping Xu Hua Yan The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury Journal of Ophthalmology |
| title | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
| title_full | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
| title_fullStr | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
| title_full_unstemmed | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
| title_short | The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury |
| title_sort | role of intravitreal anti vegf agents in rabbit eye model of open globe injury |
| url | http://dx.doi.org/10.1155/2021/5565178 |
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