CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy
Objective. To better understand attributes of ART-associated HIV-induced T-cell responses that might be therapeutically harnessed. Methods. CD8+ T-cell responses were evaluated in some HIV-1 chronically infected participants of the fixed duration STI substudy of the DART trial. Magnitudes, breadths,...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | AIDS Research and Treatment |
| Online Access: | http://dx.doi.org/10.1155/2011/875028 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565672495808512 |
|---|---|
| author | Jennifer Serwanga Susan Mugaba Auma Betty Edward Pimego Sarah Walker Paula Munderi Charles Gilks Frances Gotch Heiner Grosskurth Pontiano Kaleebu |
| author_facet | Jennifer Serwanga Susan Mugaba Auma Betty Edward Pimego Sarah Walker Paula Munderi Charles Gilks Frances Gotch Heiner Grosskurth Pontiano Kaleebu |
| author_sort | Jennifer Serwanga |
| collection | DOAJ |
| description | Objective. To better understand attributes of ART-associated HIV-induced T-cell responses that might be therapeutically harnessed.
Methods. CD8+ T-cell responses were evaluated in some HIV-1 chronically infected participants of the fixed duration STI substudy of the DART trial. Magnitudes, breadths, and functionality of IFN-γ and Perforin responses were compared in STI (n=42) and continuous treatment (CT) (n=46) before and after a single STI cycle when the DART STI trial was stopped early due to inferior clinical outcome in STI participants.
Results. STI and CT had comparable magnitudes and breadths of monofunctional CD8+IFNγ+ and CD8+Perforin+ responses. However, STI was associated with significant decline in breadth of bi-functional (CD8+IFNγ+Perforin+) responses; P=.02, Mann-Whitney test.
Conclusions. STI in individuals initiated onto ART at <200 CD4+ T-cell counts/μl significantly reduced occurrence of bifunctional CD8+IFNγ+/Perforin+ responses. These data add to others that found no evidence to support STI as a strategy to improve HIV-specific immunity during ART. |
| format | Article |
| id | doaj-art-c1a6d59df54e466aba9361f334649418 |
| institution | Kabale University |
| issn | 2090-1240 2090-1259 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | AIDS Research and Treatment |
| spelling | doaj-art-c1a6d59df54e466aba9361f3346494182025-02-03T01:07:00ZengWileyAIDS Research and Treatment2090-12402090-12592011-01-01201110.1155/2011/875028875028CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI SubstudyJennifer Serwanga0Susan Mugaba1Auma Betty2Edward Pimego3Sarah Walker4Paula Munderi5Charles Gilks6Frances Gotch7Heiner Grosskurth8Pontiano Kaleebu9MRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaMRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaMRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaMRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaMRC Clinical Trials Unit, 222 Euston Road, London NW1 2DA, UKMRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaImperial College London, South Kensington Campus, London SW7 2AZ, UKDepartment of Immunology, Imperial College, Chelsea and Westminster Hospital, London SW10 9NH, UKMRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaMRC/UVRI Uganda Research Unit on AIDS, 51-59 Nakiwogo Road, Entebbe, UgandaObjective. To better understand attributes of ART-associated HIV-induced T-cell responses that might be therapeutically harnessed. Methods. CD8+ T-cell responses were evaluated in some HIV-1 chronically infected participants of the fixed duration STI substudy of the DART trial. Magnitudes, breadths, and functionality of IFN-γ and Perforin responses were compared in STI (n=42) and continuous treatment (CT) (n=46) before and after a single STI cycle when the DART STI trial was stopped early due to inferior clinical outcome in STI participants. Results. STI and CT had comparable magnitudes and breadths of monofunctional CD8+IFNγ+ and CD8+Perforin+ responses. However, STI was associated with significant decline in breadth of bi-functional (CD8+IFNγ+Perforin+) responses; P=.02, Mann-Whitney test. Conclusions. STI in individuals initiated onto ART at <200 CD4+ T-cell counts/μl significantly reduced occurrence of bifunctional CD8+IFNγ+/Perforin+ responses. These data add to others that found no evidence to support STI as a strategy to improve HIV-specific immunity during ART.http://dx.doi.org/10.1155/2011/875028 |
| spellingShingle | Jennifer Serwanga Susan Mugaba Auma Betty Edward Pimego Sarah Walker Paula Munderi Charles Gilks Frances Gotch Heiner Grosskurth Pontiano Kaleebu CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy AIDS Research and Treatment |
| title | CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy |
| title_full | CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy |
| title_fullStr | CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy |
| title_full_unstemmed | CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy |
| title_short | CD8+ T-Cell Responses before and after Structured Treatment Interruption in Ugandan Adults Who Initiated ART with CD4+ T Cells <200 Cell/μL: The DART Trial STI Substudy |
| title_sort | cd8 t cell responses before and after structured treatment interruption in ugandan adults who initiated art with cd4 t cells 200 cell μl the dart trial sti substudy |
| url | http://dx.doi.org/10.1155/2011/875028 |
| work_keys_str_mv | AT jenniferserwanga cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT susanmugaba cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT aumabetty cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT edwardpimego cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT sarahwalker cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT paulamunderi cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT charlesgilks cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT francesgotch cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT heinergrosskurth cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy AT pontianokaleebu cd8tcellresponsesbeforeandafterstructuredtreatmentinterruptioninugandanadultswhoinitiatedartwithcd4tcells200cellmlthedarttrialstisubstudy |