Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat
Abstract Peripheral nerve injury (PNI) is a common condition that leads to the partial loss of function in the sensory, motor, and autonomic nervous systems. The peripheral nervous system has an inherent capacity to regenerate after injury and re-innervate its target organs, but full functional reco...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
|
| Series: | BMC Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12893-025-02869-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850201517030637568 |
|---|---|
| author | Wei Zhao Kun-Xiu Song Bing-Dong Ma Yong-Tao Liu Guang-Chao Sun Yong Chai |
| author_facet | Wei Zhao Kun-Xiu Song Bing-Dong Ma Yong-Tao Liu Guang-Chao Sun Yong Chai |
| author_sort | Wei Zhao |
| collection | DOAJ |
| description | Abstract Peripheral nerve injury (PNI) is a common condition that leads to the partial loss of function in the sensory, motor, and autonomic nervous systems. The peripheral nervous system has an inherent capacity to regenerate after injury and re-innervate its target organs, but full functional recovery is rare. In recent years, there has been growing interest in identifying drugs that can promote axonal regeneration and outgrowth following PNI. Epothilone B (EpoB) is an FDA-approved antineoplastic agent that promotes tubulin polymerization and enhances the stability of microtubules. Recently, the regenerative effects of EpoB in the central nervous system have garnered attention, but its potential therapeutic effects on peripheral nerve regeneration remain underexplored. This study utilized a sciatic nerve transection and anastomosis model in rats to evaluate the effects of EpoB on neuroprotein expression following nerve injury. Behavioral analysis, Masson’s trichrome staining, and immunofluorescence staining were conducted to assess the impact of EpoB on sciatic nerve regeneration. Over time, motor recovery and muscle reinnervation were observed, with Sciatic Functional Index (SFI) scores higher in the EpoB-treated group compared to the vehicle group. The expression of fibronectin (FN) was significantly lower in the EpoB group, while the expression of Tau, neurofilament-M (NF-M), and growth-associated protein-43 (GAP-43) was significantly higher. In conclusion, EpoB treatment significantly increases the expression of Tau, NF-M, and GAP-43, suggesting a positive effect on axonal regeneration and repair. |
| format | Article |
| id | doaj-art-c1a2e2fdbef24df19dfd22879854d771 |
| institution | OA Journals |
| issn | 1471-2482 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Surgery |
| spelling | doaj-art-c1a2e2fdbef24df19dfd22879854d7712025-08-20T02:11:59ZengBMCBMC Surgery1471-24822025-04-012511910.1186/s12893-025-02869-xEffect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the ratWei Zhao0Kun-Xiu Song1Bing-Dong Ma2Yong-Tao Liu3Guang-Chao Sun4Yong Chai5Department of Hand (micro) Surgery, Binzhou Medical University HospitalDepartment of Hand (micro) Surgery, Binzhou Medical University HospitalDepartment of Hand (micro) Surgery, Binzhou Medical University HospitalDepartment of Hand (micro) Surgery, Binzhou Medical University HospitalDepartment of Foot and ankle Surgery, Binzhou Medical University HospitalDepartment of Anatomy, Binzhou Medical UniversityAbstract Peripheral nerve injury (PNI) is a common condition that leads to the partial loss of function in the sensory, motor, and autonomic nervous systems. The peripheral nervous system has an inherent capacity to regenerate after injury and re-innervate its target organs, but full functional recovery is rare. In recent years, there has been growing interest in identifying drugs that can promote axonal regeneration and outgrowth following PNI. Epothilone B (EpoB) is an FDA-approved antineoplastic agent that promotes tubulin polymerization and enhances the stability of microtubules. Recently, the regenerative effects of EpoB in the central nervous system have garnered attention, but its potential therapeutic effects on peripheral nerve regeneration remain underexplored. This study utilized a sciatic nerve transection and anastomosis model in rats to evaluate the effects of EpoB on neuroprotein expression following nerve injury. Behavioral analysis, Masson’s trichrome staining, and immunofluorescence staining were conducted to assess the impact of EpoB on sciatic nerve regeneration. Over time, motor recovery and muscle reinnervation were observed, with Sciatic Functional Index (SFI) scores higher in the EpoB-treated group compared to the vehicle group. The expression of fibronectin (FN) was significantly lower in the EpoB group, while the expression of Tau, neurofilament-M (NF-M), and growth-associated protein-43 (GAP-43) was significantly higher. In conclusion, EpoB treatment significantly increases the expression of Tau, NF-M, and GAP-43, suggesting a positive effect on axonal regeneration and repair.https://doi.org/10.1186/s12893-025-02869-xSciatic nerve transection anastomosisPeripheral nerve injuryEpothilone BFibronectinTauneurofilament-M |
| spellingShingle | Wei Zhao Kun-Xiu Song Bing-Dong Ma Yong-Tao Liu Guang-Chao Sun Yong Chai Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat BMC Surgery Sciatic nerve transection anastomosis Peripheral nerve injury Epothilone B Fibronectin Tau neurofilament-M |
| title | Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat |
| title_full | Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat |
| title_fullStr | Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat |
| title_full_unstemmed | Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat |
| title_short | Effect of epothilone B on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat |
| title_sort | effect of epothilone b on the expression of neuroproteins after anastomosis of the sciatic nerve transection in the rat |
| topic | Sciatic nerve transection anastomosis Peripheral nerve injury Epothilone B Fibronectin Tau neurofilament-M |
| url | https://doi.org/10.1186/s12893-025-02869-x |
| work_keys_str_mv | AT weizhao effectofepothilonebontheexpressionofneuroproteinsafteranastomosisofthesciaticnervetransectionintherat AT kunxiusong effectofepothilonebontheexpressionofneuroproteinsafteranastomosisofthesciaticnervetransectionintherat AT bingdongma effectofepothilonebontheexpressionofneuroproteinsafteranastomosisofthesciaticnervetransectionintherat AT yongtaoliu effectofepothilonebontheexpressionofneuroproteinsafteranastomosisofthesciaticnervetransectionintherat AT guangchaosun effectofepothilonebontheexpressionofneuroproteinsafteranastomosisofthesciaticnervetransectionintherat AT yongchai effectofepothilonebontheexpressionofneuroproteinsafteranastomosisofthesciaticnervetransectionintherat |