Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China
Objective This study aimed to evaluate the efficiency of nanopore sequencing for the early diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid and compared it with acid-fast bacilli (AFB) smear, mycobacterial growth indicator tube culture and Xpert Mycobacterium tuberculosis (MTB)/ri...
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BMJ Publishing Group
2024-06-01
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| Series: | BMJ Open |
| Online Access: | https://bmjopen.bmj.com/content/14/6/e080904.full |
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| author | Lihong Zhou Xingwu Zou Yan Yong Qin Hu |
| author_facet | Lihong Zhou Xingwu Zou Yan Yong Qin Hu |
| author_sort | Lihong Zhou |
| collection | DOAJ |
| description | Objective This study aimed to evaluate the efficiency of nanopore sequencing for the early diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid and compared it with acid-fast bacilli (AFB) smear, mycobacterial growth indicator tube culture and Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF).Design Single-centre retrospective study.Setting The Tuberculosis Diagnosis and Treatment Center of Zhejiang Chinese and Western Medicine Integrated Hospital.Participants We enrolled 64 adult patients with presumptive TBM admitted to our hospital from August 2021 to August 2023.Methods We calculated the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of AFB smear, culture, Xpert MTB/RIF and nanopore sequencing to evaluate their diagnostic efficacy compared with a composite reference standard for TBM.Results Among these 64 patients, all tested negative for TBM by AFB smear. The sensitivity, specificity, PPV and NPV were 11.11%, 100%, 100% and 32.2% for culture, 13.33%, 100%, 100% and 2.76% for Xpert MTB/RIF, and 77.78%, 100%, 100% and 65.52% for nanopore sequencing, respectively.Conclusion The diagnostic accuracy of the nanopore sequencing test was significantly higher than that of conventional testing methods used to detect TBM. |
| format | Article |
| id | doaj-art-c18d18b9c02d429b9df3e8cada924f95 |
| institution | DOAJ |
| issn | 2044-6055 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open |
| spelling | doaj-art-c18d18b9c02d429b9df3e8cada924f952025-08-20T02:40:30ZengBMJ Publishing GroupBMJ Open2044-60552024-06-0114610.1136/bmjopen-2023-080904Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in ChinaLihong Zhou0Xingwu Zou1Yan Yong2Qin Hu3Tuberculosis Diagnosis and Treatment Center, Integrated Traditional Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou, ChinaTuberculosis Diagnosis and Treatment Center, Integrated Traditional Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou, ChinaTuberculosis Diagnosis and Treatment Center, Integrated Traditional Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou, ChinaTuberculosis Diagnosis and Treatment Center, Integrated Traditional Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou, ChinaObjective This study aimed to evaluate the efficiency of nanopore sequencing for the early diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid and compared it with acid-fast bacilli (AFB) smear, mycobacterial growth indicator tube culture and Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF).Design Single-centre retrospective study.Setting The Tuberculosis Diagnosis and Treatment Center of Zhejiang Chinese and Western Medicine Integrated Hospital.Participants We enrolled 64 adult patients with presumptive TBM admitted to our hospital from August 2021 to August 2023.Methods We calculated the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of AFB smear, culture, Xpert MTB/RIF and nanopore sequencing to evaluate their diagnostic efficacy compared with a composite reference standard for TBM.Results Among these 64 patients, all tested negative for TBM by AFB smear. The sensitivity, specificity, PPV and NPV were 11.11%, 100%, 100% and 32.2% for culture, 13.33%, 100%, 100% and 2.76% for Xpert MTB/RIF, and 77.78%, 100%, 100% and 65.52% for nanopore sequencing, respectively.Conclusion The diagnostic accuracy of the nanopore sequencing test was significantly higher than that of conventional testing methods used to detect TBM.https://bmjopen.bmj.com/content/14/6/e080904.full |
| spellingShingle | Lihong Zhou Xingwu Zou Yan Yong Qin Hu Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China BMJ Open |
| title | Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China |
| title_full | Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China |
| title_fullStr | Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China |
| title_full_unstemmed | Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China |
| title_short | Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China |
| title_sort | using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis a retrospective cohort study in china |
| url | https://bmjopen.bmj.com/content/14/6/e080904.full |
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