Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy
Xiaoyu Ma, Ni Chen, Peiyuan Zeng, Yuqian He, Tao Zhang, Yu Lu, Ziyu Li, Jin Xu, Jingcan You, Youkun Zheng, Liqun Wang, Mao Luo, Jianbo Wu Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education; Laboratory for Cardiovascular Pharmacology, Department of Pharmacol...
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Dove Medical Press
2025-02-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/hypericum-perforatum-derived-exosomes-like-nanovesicles-a-novel-natura-peer-reviewed-fulltext-article-IJN |
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| author | Ma X Chen N Zeng P He Y Zhang T Lu Y Li Z Xu J You J Zheng Y Wang L Luo M Wu J |
| author_facet | Ma X Chen N Zeng P He Y Zhang T Lu Y Li Z Xu J You J Zheng Y Wang L Luo M Wu J |
| author_sort | Ma X |
| collection | DOAJ |
| description | Xiaoyu Ma, Ni Chen, Peiyuan Zeng, Yuqian He, Tao Zhang, Yu Lu, Ziyu Li, Jin Xu, Jingcan You, Youkun Zheng, Liqun Wang, Mao Luo, Jianbo Wu Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education; Laboratory for Cardiovascular Pharmacology, Department of Pharmacology, School of Pharmacy; Luzhou Municipal Key Laboratory of Thrombosis and Vascular Biology Southwest Medical University, Luzhou, 646000, People’s Republic of ChinaCorrespondence: Jianbo Wu, Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education, Southwest Medical University, Luzhou, Sichuan, 646000, People’s Republic of China, Tel/Fax +0086-830-3161702, Email jbwucn1996@yahoo.comBackground: Natural photosensitizers hold potential for photodynamic therapy (PDT) but are often limited by poor visible light absorption. Plant-derived exosome-like nanovesicles offer an innovative platform for enhancing photosensitizer performance.Methods: Hypericum perforatum-derived nanovesicles (HPDENs) were characterized using electron microscopy, dynamic light scattering, and proteomic and miRNA sequencing. High-performance liquid chromatography confirmed hypericin content. PDT efficacy was assessed in vitro and in vivo.Results: HPDENs exhibited robust photosensitizing properties, generating reactive oxygen species (ROS) through both Type I and Type II pathways upon light activation. In vitro, HPDENs showed light dose-dependent cytotoxicity against human melanoma cells, characterized by elevated ROS production and apoptosis induction. In vivo, HPDEN-mediated PDT significantly suppressed tumor growth and induced extensive tumor necrosis, with no observable toxicity to major organs.Conclusion: HPDENs represent a novel plant-derived photosensitizer with dual ROS generation pathways and significant therapeutic efficacy, providing a promising platform for enhancing photodynamic therapy.Keywords: Hypericum perforatum-derived exosomes-like nanovesicles, photosensitizer, photodynamic therapy, reactive oxygen species, apoptosis |
| format | Article |
| id | doaj-art-c1837cbc99f54daa9e575f73e64f8bb7 |
| institution | Kabale University |
| issn | 1178-2013 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj-art-c1837cbc99f54daa9e575f73e64f8bb72025-02-04T17:15:41ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-02-01Volume 201529154199854Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic TherapyMa XChen NZeng PHe YZhang TLu YLi ZXu JYou JZheng YWang LLuo MWu JXiaoyu Ma, Ni Chen, Peiyuan Zeng, Yuqian He, Tao Zhang, Yu Lu, Ziyu Li, Jin Xu, Jingcan You, Youkun Zheng, Liqun Wang, Mao Luo, Jianbo Wu Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education; Laboratory for Cardiovascular Pharmacology, Department of Pharmacology, School of Pharmacy; Luzhou Municipal Key Laboratory of Thrombosis and Vascular Biology Southwest Medical University, Luzhou, 646000, People’s Republic of ChinaCorrespondence: Jianbo Wu, Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education, Southwest Medical University, Luzhou, Sichuan, 646000, People’s Republic of China, Tel/Fax +0086-830-3161702, Email jbwucn1996@yahoo.comBackground: Natural photosensitizers hold potential for photodynamic therapy (PDT) but are often limited by poor visible light absorption. Plant-derived exosome-like nanovesicles offer an innovative platform for enhancing photosensitizer performance.Methods: Hypericum perforatum-derived nanovesicles (HPDENs) were characterized using electron microscopy, dynamic light scattering, and proteomic and miRNA sequencing. High-performance liquid chromatography confirmed hypericin content. PDT efficacy was assessed in vitro and in vivo.Results: HPDENs exhibited robust photosensitizing properties, generating reactive oxygen species (ROS) through both Type I and Type II pathways upon light activation. In vitro, HPDENs showed light dose-dependent cytotoxicity against human melanoma cells, characterized by elevated ROS production and apoptosis induction. In vivo, HPDEN-mediated PDT significantly suppressed tumor growth and induced extensive tumor necrosis, with no observable toxicity to major organs.Conclusion: HPDENs represent a novel plant-derived photosensitizer with dual ROS generation pathways and significant therapeutic efficacy, providing a promising platform for enhancing photodynamic therapy.Keywords: Hypericum perforatum-derived exosomes-like nanovesicles, photosensitizer, photodynamic therapy, reactive oxygen species, apoptosishttps://www.dovepress.com/hypericum-perforatum-derived-exosomes-like-nanovesicles-a-novel-natura-peer-reviewed-fulltext-article-IJNhypericum perforatum-derived exosomes-like nanovesiclesphotosensitizerphotodynamic therapyreactive oxygen speciesapoptosis |
| spellingShingle | Ma X Chen N Zeng P He Y Zhang T Lu Y Li Z Xu J You J Zheng Y Wang L Luo M Wu J Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy International Journal of Nanomedicine hypericum perforatum-derived exosomes-like nanovesicles photosensitizer photodynamic therapy reactive oxygen species apoptosis |
| title | Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy |
| title_full | Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy |
| title_fullStr | Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy |
| title_full_unstemmed | Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy |
| title_short | Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy |
| title_sort | hypericum perforatum derived exosomes like nanovesicles a novel natural photosensitizer for effective tumor photodynamic therapy |
| topic | hypericum perforatum-derived exosomes-like nanovesicles photosensitizer photodynamic therapy reactive oxygen species apoptosis |
| url | https://www.dovepress.com/hypericum-perforatum-derived-exosomes-like-nanovesicles-a-novel-natura-peer-reviewed-fulltext-article-IJN |
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