The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression
Cellular immunosuppression appears to be involved in sepsis and sepsis-induced multiple organ dysfunction syndrome (MODS). Recent evidence showed that parenteral vitamin C (Vit C) had the ability to attenuate sepsis and sepsis-induced MODS. Herein, we investigated the impact of parenteral Vit C on c...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/4024672 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552095962628096 |
|---|---|
| author | Yu-Lei Gao Bin Lu Jian-Hua Zhai Yan-Cun Liu Hai-Xia Qi Ying Yao Yan-Fen Chai Song-Tao Shou |
| author_facet | Yu-Lei Gao Bin Lu Jian-Hua Zhai Yan-Cun Liu Hai-Xia Qi Ying Yao Yan-Fen Chai Song-Tao Shou |
| author_sort | Yu-Lei Gao |
| collection | DOAJ |
| description | Cellular immunosuppression appears to be involved in sepsis and sepsis-induced multiple organ dysfunction syndrome (MODS). Recent evidence showed that parenteral vitamin C (Vit C) had the ability to attenuate sepsis and sepsis-induced MODS. Herein, we investigated the impact of parenteral Vit C on cellular immunosuppression and the therapeutic value in sepsis. Using cecal ligation and puncture (CLP), sepsis was induced in WT and Gulo−/− mice followed with 200 mg/Kg parenteral Vit C administration. The immunologic functions of CD4+CD25+ regulatory T cells (Tregs) and CD4+CD25− T cells, as well as the organ functions, were determined. Administration of parenteral Vit C per se markedly improved the outcome of sepsis and sepsis-induced MODS of WT and Gulo−/− mice. The negative immunoregulation of Tregs was inhibited, mainly including inhibiting the expression of forkhead helix transcription factor- (Foxp-) 3, cytotoxic T lymphocyte associated antigen- (CTLA-) 4, membrane associated transforming growth factor-β (TGF-βm+), and the secretion of inhibitory cytokines [including TGF-β and interleukin- (IL-) 10], as well as CD4+ T cells-mediated cellular immunosuppression which was improved by parenteral Vit C in WT and Gulo−/− septic mice. These results suggested that parenteral Vit C has the ability to improve the outcome of sepsis and sepsis-induced MODS and is associated with improvement in cellular immunosuppression. |
| format | Article |
| id | doaj-art-c180210dde1f4a3bb5be9e9f8fca2896 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c180210dde1f4a3bb5be9e9f8fca28962025-02-03T05:59:33ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/40246724024672The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular ImmunosuppressionYu-Lei Gao0Bin Lu1Jian-Hua Zhai2Yan-Cun Liu3Hai-Xia Qi4Ying Yao5Yan-Fen Chai6Song-Tao Shou7Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, ChinaCellular immunosuppression appears to be involved in sepsis and sepsis-induced multiple organ dysfunction syndrome (MODS). Recent evidence showed that parenteral vitamin C (Vit C) had the ability to attenuate sepsis and sepsis-induced MODS. Herein, we investigated the impact of parenteral Vit C on cellular immunosuppression and the therapeutic value in sepsis. Using cecal ligation and puncture (CLP), sepsis was induced in WT and Gulo−/− mice followed with 200 mg/Kg parenteral Vit C administration. The immunologic functions of CD4+CD25+ regulatory T cells (Tregs) and CD4+CD25− T cells, as well as the organ functions, were determined. Administration of parenteral Vit C per se markedly improved the outcome of sepsis and sepsis-induced MODS of WT and Gulo−/− mice. The negative immunoregulation of Tregs was inhibited, mainly including inhibiting the expression of forkhead helix transcription factor- (Foxp-) 3, cytotoxic T lymphocyte associated antigen- (CTLA-) 4, membrane associated transforming growth factor-β (TGF-βm+), and the secretion of inhibitory cytokines [including TGF-β and interleukin- (IL-) 10], as well as CD4+ T cells-mediated cellular immunosuppression which was improved by parenteral Vit C in WT and Gulo−/− septic mice. These results suggested that parenteral Vit C has the ability to improve the outcome of sepsis and sepsis-induced MODS and is associated with improvement in cellular immunosuppression.http://dx.doi.org/10.1155/2017/4024672 |
| spellingShingle | Yu-Lei Gao Bin Lu Jian-Hua Zhai Yan-Cun Liu Hai-Xia Qi Ying Yao Yan-Fen Chai Song-Tao Shou The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression Mediators of Inflammation |
| title | The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression |
| title_full | The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression |
| title_fullStr | The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression |
| title_full_unstemmed | The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression |
| title_short | The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression |
| title_sort | parenteral vitamin c improves sepsis and sepsis induced multiple organ dysfunction syndrome via preventing cellular immunosuppression |
| url | http://dx.doi.org/10.1155/2017/4024672 |
| work_keys_str_mv | AT yuleigao theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT binlu theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT jianhuazhai theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yancunliu theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT haixiaqi theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yingyao theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yanfenchai theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT songtaoshou theparenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yuleigao parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT binlu parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT jianhuazhai parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yancunliu parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT haixiaqi parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yingyao parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT yanfenchai parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression AT songtaoshou parenteralvitamincimprovessepsisandsepsisinducedmultipleorgandysfunctionsyndromeviapreventingcellularimmunosuppression |