Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates
This study explores the optimisation of rearing substrates for black soldier fly larvae (BSFL). First, the ideal dry matter content of substrates was determined, comparing the standard 30% dry matter (DM) with substrates hydrated to their maximum water holding capacity (WHC). Substrates at maximal W...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/16/1/77 |
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| author | Laurens Broeckx Lotte Frooninckx Siebe Berrens Sarah Goossens Carmen ter Heide Ann Wuyts Mariève Dallaire-Lamontagne Sabine Van Miert |
| author_facet | Laurens Broeckx Lotte Frooninckx Siebe Berrens Sarah Goossens Carmen ter Heide Ann Wuyts Mariève Dallaire-Lamontagne Sabine Van Miert |
| author_sort | Laurens Broeckx |
| collection | DOAJ |
| description | This study explores the optimisation of rearing substrates for black soldier fly larvae (BSFL). First, the ideal dry matter content of substrates was determined, comparing the standard 30% dry matter (DM) with substrates hydrated to their maximum water holding capacity (WHC). Substrates at maximal WHC yielded significantly higher larval survival rates (<i>p</i> = 0.0006). Consequently, the WHC approach was adopted for further experiments. Using these hydrated artificial substrates, fractional factorial designs based on central composite and Box–Behnken designs were employed to assess the impact of macronutrient composition on bioconversion efficiency. The results demonstrated significant main, interaction, and quadratic effects on bioconversion efficiency. Validation with real-life substrates of varied protein content, including indigestible feather meal, affirmed the predictive model’s accuracy after accounting for protein source digestibility. This research underscores the importance of optimal hydration and macronutrient composition in enhancing BSFL growth and bioconversion efficiency. |
| format | Article |
| id | doaj-art-c17b02a9f89e45499331dd9af33af5f2 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-c17b02a9f89e45499331dd9af33af5f22025-01-24T13:35:48ZengMDPI AGInsects2075-44502025-01-011617710.3390/insects16010077Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial SubstratesLaurens Broeckx0Lotte Frooninckx1Siebe Berrens2Sarah Goossens3Carmen ter Heide4Ann Wuyts5Mariève Dallaire-Lamontagne6Sabine Van Miert7Centre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumCentre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumCentre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumCentre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumCentre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumCentre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumDépartement des Sciences Animales, Faculté des Sciences de L’Agriculture et de L’Alimentation, Université Laval, 2425 rue de l’Agriculture, Québec, QC G1V 0A6, CanadaCentre of Expertise Sustainable Biomass and Chemistry, Campus Geel, Thomas More University of Applied Sciences, Kleinhoefstraat 4, 2440 Geel, BelgiumThis study explores the optimisation of rearing substrates for black soldier fly larvae (BSFL). First, the ideal dry matter content of substrates was determined, comparing the standard 30% dry matter (DM) with substrates hydrated to their maximum water holding capacity (WHC). Substrates at maximal WHC yielded significantly higher larval survival rates (<i>p</i> = 0.0006). Consequently, the WHC approach was adopted for further experiments. Using these hydrated artificial substrates, fractional factorial designs based on central composite and Box–Behnken designs were employed to assess the impact of macronutrient composition on bioconversion efficiency. The results demonstrated significant main, interaction, and quadratic effects on bioconversion efficiency. Validation with real-life substrates of varied protein content, including indigestible feather meal, affirmed the predictive model’s accuracy after accounting for protein source digestibility. This research underscores the importance of optimal hydration and macronutrient composition in enhancing BSFL growth and bioconversion efficiency.https://www.mdpi.com/2075-4450/16/1/77black soldier flymacronutrientsmodellingbioconversionartificial substrates |
| spellingShingle | Laurens Broeckx Lotte Frooninckx Siebe Berrens Sarah Goossens Carmen ter Heide Ann Wuyts Mariève Dallaire-Lamontagne Sabine Van Miert Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates Insects black soldier fly macronutrients modelling bioconversion artificial substrates |
| title | Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates |
| title_full | Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates |
| title_fullStr | Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates |
| title_full_unstemmed | Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates |
| title_short | Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Through Artificial Substrates |
| title_sort | macronutrient based predictive modelling of bioconversion efficiency in black soldier fly larvae i hermetia illucens i through artificial substrates |
| topic | black soldier fly macronutrients modelling bioconversion artificial substrates |
| url | https://www.mdpi.com/2075-4450/16/1/77 |
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