G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates
Abstract Background Chromoblastomycosis is the World Health Organization (WHO)-recognized fungal implantation disease that eventually leads to severe mutilation. Cladophialophora carrionii (C. carrionii) is one of the agents. However, the pathogenesis of C. carrionii is not fully investigated yet. M...
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BMC
2024-03-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-023-00134-5 |
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| author | Dongmei Shi Zhiya Yang Wanqing Liao Chen Liu Liang Zhao Huilin Su Xiaodong Wang Huan Mei Min Chen Yinggai Song Sybren de Hoog Shuwen Deng |
| author_facet | Dongmei Shi Zhiya Yang Wanqing Liao Chen Liu Liang Zhao Huilin Su Xiaodong Wang Huan Mei Min Chen Yinggai Song Sybren de Hoog Shuwen Deng |
| author_sort | Dongmei Shi |
| collection | DOAJ |
| description | Abstract Background Chromoblastomycosis is the World Health Organization (WHO)-recognized fungal implantation disease that eventually leads to severe mutilation. Cladophialophora carrionii (C. carrionii) is one of the agents. However, the pathogenesis of C. carrionii is not fully investigated yet. Methods We investigated the pathogenic potential of the fungus in a Galleria mellonella (G. mellonella) larvae infection model. Six strains of C. carrionii, and three of its environmental relative C. yegresii were tested. The G. mellonella model was also applied to determine antifungal efficacy of amphotericin B, itraconazole, voriconazole, posaconazole, and terbinafine. Results All strains were able to infect the larvae, but virulence potentials were strain-specific and showed no correlation with clinical background of the respective isolate. Survival of larvae also varied with infection dose, and with growth speed and melanization of the fungus. Posaconazole and voriconazole exhibited best activity against Cladophialophora, followed by itraconazole and terbinafine, while limited efficacy was seen for amphotericin B. Conclusion Infection behavior deviates significantly between strains. In vitro antifungal susceptibility of tested strains only partly explained the limited treatment efficacy in vivo. |
| format | Article |
| id | doaj-art-c171124b90704b0f9e41f28fc520598d |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-c171124b90704b0f9e41f28fc520598d2025-02-03T09:04:30ZengBMCIMA Fungus2210-63592024-03-0115111010.1186/s43008-023-00134-5G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolatesDongmei Shi0Zhiya Yang1Wanqing Liao2Chen Liu3Liang Zhao4Huilin Su5Xiaodong Wang6Huan Mei7Min Chen8Yinggai Song9Sybren de Hoog10Shuwen Deng11Department of Dermatology & Laboratory of Medical Mycology, Jining No. 1 People’s HospitalThe Laboratory of Medical Mycology, Jining No. 1 People’s HospitalDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng HospitalThe Laboratory of Medical Mycology, Jining No. 1 People’s HospitalKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou & Guizhou Talent Base for Microbiology and Human Health, School of Basic Medical Sciences, Guizhou Medical UniversityDepartment of Dermatology, The First Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Dermatology, the First Affiliated Hospital of Xinjiang Medical UniversityInstitute of Dermatology, Chinese Academy of Medical ScienceDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng HospitalDepartment of Dermatology and Venereology, First Hospital in Peking UniversityCenter of Expertise in Mycology of Radboud University Medical Center, Canisius Wilhelmina HospitalDepartment of Medical Microbiology, The People’s Hospital of Suzhou New DistrictAbstract Background Chromoblastomycosis is the World Health Organization (WHO)-recognized fungal implantation disease that eventually leads to severe mutilation. Cladophialophora carrionii (C. carrionii) is one of the agents. However, the pathogenesis of C. carrionii is not fully investigated yet. Methods We investigated the pathogenic potential of the fungus in a Galleria mellonella (G. mellonella) larvae infection model. Six strains of C. carrionii, and three of its environmental relative C. yegresii were tested. The G. mellonella model was also applied to determine antifungal efficacy of amphotericin B, itraconazole, voriconazole, posaconazole, and terbinafine. Results All strains were able to infect the larvae, but virulence potentials were strain-specific and showed no correlation with clinical background of the respective isolate. Survival of larvae also varied with infection dose, and with growth speed and melanization of the fungus. Posaconazole and voriconazole exhibited best activity against Cladophialophora, followed by itraconazole and terbinafine, while limited efficacy was seen for amphotericin B. Conclusion Infection behavior deviates significantly between strains. In vitro antifungal susceptibility of tested strains only partly explained the limited treatment efficacy in vivo.https://doi.org/10.1186/s43008-023-00134-5Galleria mellonellaChromoblastomycosisCladophialophora carrioniiVirulenceAntifungal efficacy in vivo |
| spellingShingle | Dongmei Shi Zhiya Yang Wanqing Liao Chen Liu Liang Zhao Huilin Su Xiaodong Wang Huan Mei Min Chen Yinggai Song Sybren de Hoog Shuwen Deng G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates IMA Fungus Galleria mellonella Chromoblastomycosis Cladophialophora carrionii Virulence Antifungal efficacy in vivo |
| title | G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates |
| title_full | G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates |
| title_fullStr | G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates |
| title_full_unstemmed | G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates |
| title_short | G alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates |
| title_sort | g alleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates |
| topic | Galleria mellonella Chromoblastomycosis Cladophialophora carrionii Virulence Antifungal efficacy in vivo |
| url | https://doi.org/10.1186/s43008-023-00134-5 |
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