Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly
Abstract Protein fibers are ideal alternatives to synthetic polymers due to their unique mechanical properties, biocompatibility, and sustainability. However, engineering biomimetic protein fibers with high mechanical properties remains challenging, particularly in mimicking the high molecular weigh...
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| Format: | Article |
| Language: | English |
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Wiley
2024-12-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202410199 |
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| author | Ming Li Dawen Qin Jing Chen Bo Jia Zheng Wei Yi Zhang Wenhao Cheng Qianqian Liu Fan Wang Jingjing Li Hongjie Zhang Kai Liu |
| author_facet | Ming Li Dawen Qin Jing Chen Bo Jia Zheng Wei Yi Zhang Wenhao Cheng Qianqian Liu Fan Wang Jingjing Li Hongjie Zhang Kai Liu |
| author_sort | Ming Li |
| collection | DOAJ |
| description | Abstract Protein fibers are ideal alternatives to synthetic polymers due to their unique mechanical properties, biocompatibility, and sustainability. However, engineering biomimetic protein fibers with high mechanical properties remains challenging, particularly in mimicking the high molecular weight of natural proteins and regulating their complex hierarchical structures. Here, a modular design and multi‐scale assembly strategy is developed to manufacture robust protein fibers using low‐ or medium‐molecular‐weight proteins. The distinct functional and structural properties of flexible, rigid, and cross‐linked domains in modular proteins are skillfully harnessed. By regulating the ratio of rigid to flexible domains, the formation of high‐order β‐sheet crystals aligned along the fiber axis is promoted, enhancing both strength and toughness. Furthermore, the dynamic imine cross‐linking network, formed by the aldehyde‐amine condensation reaction of the cross‐linked domains, further reinforces the protein fibers. Remarkably, fibers spun from modular proteins significantly smaller than natural spidroin exhibit outstanding mechanical properties, surpassing those of protein fibers with same or even higher molecular weights. This strategy offers a promising pathway for fabricating protein fibers suitable for diverse applications. |
| format | Article |
| id | doaj-art-c135c58e204b44cea89e5d4105794c39 |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-c135c58e204b44cea89e5d4105794c392025-08-20T02:50:00ZengWileyAdvanced Science2198-38442024-12-011146n/an/a10.1002/advs.202410199Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order AssemblyMing Li0Dawen Qin1Jing Chen2Bo Jia3Zheng Wei4Yi Zhang5Wenhao Cheng6Qianqian Liu7Fan Wang8Jingjing Li9Hongjie Zhang10Kai Liu11State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaFuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100084 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaState Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 ChinaAbstract Protein fibers are ideal alternatives to synthetic polymers due to their unique mechanical properties, biocompatibility, and sustainability. However, engineering biomimetic protein fibers with high mechanical properties remains challenging, particularly in mimicking the high molecular weight of natural proteins and regulating their complex hierarchical structures. Here, a modular design and multi‐scale assembly strategy is developed to manufacture robust protein fibers using low‐ or medium‐molecular‐weight proteins. The distinct functional and structural properties of flexible, rigid, and cross‐linked domains in modular proteins are skillfully harnessed. By regulating the ratio of rigid to flexible domains, the formation of high‐order β‐sheet crystals aligned along the fiber axis is promoted, enhancing both strength and toughness. Furthermore, the dynamic imine cross‐linking network, formed by the aldehyde‐amine condensation reaction of the cross‐linked domains, further reinforces the protein fibers. Remarkably, fibers spun from modular proteins significantly smaller than natural spidroin exhibit outstanding mechanical properties, surpassing those of protein fibers with same or even higher molecular weights. This strategy offers a promising pathway for fabricating protein fibers suitable for diverse applications.https://doi.org/10.1002/advs.202410199β‐sheet assemblybiological fibersprotein engineeringresilinspidroin |
| spellingShingle | Ming Li Dawen Qin Jing Chen Bo Jia Zheng Wei Yi Zhang Wenhao Cheng Qianqian Liu Fan Wang Jingjing Li Hongjie Zhang Kai Liu Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly Advanced Science β‐sheet assembly biological fibers protein engineering resilin spidroin |
| title | Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly |
| title_full | Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly |
| title_fullStr | Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly |
| title_full_unstemmed | Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly |
| title_short | Engineered Protein Fibers with Reinforced Mechanical Properties Via β‐Sheet High‐Order Assembly |
| title_sort | engineered protein fibers with reinforced mechanical properties via β sheet high order assembly |
| topic | β‐sheet assembly biological fibers protein engineering resilin spidroin |
| url | https://doi.org/10.1002/advs.202410199 |
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