Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone
In this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial ce...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000300 |
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| author | M. M. Verheggen H. I. M. de Bont P. W. C. Adriaansen-Soeting B. J. A. Goense C. J. A. M. Tak H. C. Hoogsteden P. Th. W. van Hal M. A. Versnel |
| author_facet | M. M. Verheggen H. I. M. de Bont P. W. C. Adriaansen-Soeting B. J. A. Goense C. J. A. M. Tak H. C. Hoogsteden P. Th. W. van Hal M. A. Versnel |
| author_sort | M. M. Verheggen |
| collection | DOAJ |
| description | In this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-1β, TNF-α or LPS for 24 h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGF1α production was significantly increased. This IL-1β- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction. |
| format | Article |
| id | doaj-art-c134a8a701c743f0bc19a196498f1cb2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c134a8a701c743f0bc19a196498f1cb22025-02-03T01:27:52ZengWileyMediators of Inflammation0962-93511466-18611996-01-015321021710.1155/S0962935196000300Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasoneM. M. Verheggen0H. I. M. de Bont1P. W. C. Adriaansen-Soeting2B. J. A. Goense3C. J. A. M. Tak4H. C. Hoogsteden5P. Th. W. van Hal6M. A. Versnel7Department of Immunology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of pharmacology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of pulmonary Medicine , Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University and Academic Hospital Dijkzigt, Rotterdam, The NetherlandsIn this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-1β, TNF-α or LPS for 24 h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGF1α production was significantly increased. This IL-1β- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.http://dx.doi.org/10.1155/S0962935196000300 |
| spellingShingle | M. M. Verheggen H. I. M. de Bont P. W. C. Adriaansen-Soeting B. J. A. Goense C. J. A. M. Tak H. C. Hoogsteden P. Th. W. van Hal M. A. Versnel Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone Mediators of Inflammation |
| title | Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone |
| title_full | Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone |
| title_fullStr | Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone |
| title_full_unstemmed | Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone |
| title_short | Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone |
| title_sort | expression of lipocortins in human bronchial epithelial cells effects of il 1β tnf α lps and dexamethasone |
| url | http://dx.doi.org/10.1155/S0962935196000300 |
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