Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids
This study investigates the resistance of 17 apricot hybrids to Potyvirus plumpoxi (PPV) with the use of phenotyping, serological assays [double-antibody-sandwich enzyme-linked immunosorbent assay (DAS-ELISA)], and molecular marker analyses. The expression of symptoms expression was evaluated on fou...
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American Society for Horticultural Science (ASHS)
2025-01-01
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| author | Eliška Zezulová Tomáš Kiss Kateřina Baránková Tomáš Nečas |
| author_facet | Eliška Zezulová Tomáš Kiss Kateřina Baránková Tomáš Nečas |
| author_sort | Eliška Zezulová |
| collection | DOAJ |
| description | This study investigates the resistance of 17 apricot hybrids to Potyvirus plumpoxi (PPV) with the use of phenotyping, serological assays [double-antibody-sandwich enzyme-linked immunosorbent assay (DAS-ELISA)], and molecular marker analyses. The expression of symptoms expression was evaluated on four occasions throughout the course of the experiment, while the presence of the virus in the plant was diagnosed by DAS-ELISA. In addition, the genotyping with simple sequence repeat (SSR) markers (PGS1.21, PGS1.23) and ParPMC2 allele-specific polymerase chain reaction (PCR) for the purpose of evaluating PPV resistance. A total of five new apricot hybrids were evaluated as resistant to PPV, while five hybrids exhibited resistance despite the presence of some PPV symptoms. In addition, three hybrids demonstrated genotype-phenotype incongruences (GPIs). The statistical analysis revealed a significant relation between PPV-positive plants and the absence of resistance-associated alleles, confirming that allelic composition is strongly associated with phenotypic resistance. The average value of symptoms evaluation was 0.3 ± 0.1 for resistant hybrids and 1.7 ± 0.3 and 1.5 ± 0.4 for susceptible hybrids evaluated by PGS1.21 and PGS1.23, respectively. The Tukey’s honestly significant difference (HSD) test showed the significant difference between genotypes evaluated by ParPMC2 marker as “RR” and “RS” (resistant) from “SS” (susceptible). However, discrepancies in hybrid resistance evaluation highlight the complexity of inheritance and the potential errors in genotyping. These results indicate that the most reliable method for evaluating PPV resistance is the time-consuming phenotyping method. Conversely, the use of SSR markers greatly improves the effectiveness of breeding |
| format | Article |
| id | doaj-art-c118d0b91fef490d93ca87447cbdc727 |
| institution | Kabale University |
| issn | 2327-9834 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Horticultural Science (ASHS) |
| record_format | Article |
| series | HortScience |
| spelling | doaj-art-c118d0b91fef490d93ca87447cbdc7272025-01-29T16:47:16ZengAmerican Society for Horticultural Science (ASHS)HortScience2327-98342025-01-01602https://doi.org/10.21273/HORTSCI18348-24Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot HybridsEliška Zezulová0Tomáš Kiss1Kateřina Baránková2Tomáš Nečas3Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicFaculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicFaculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicFaculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicThis study investigates the resistance of 17 apricot hybrids to Potyvirus plumpoxi (PPV) with the use of phenotyping, serological assays [double-antibody-sandwich enzyme-linked immunosorbent assay (DAS-ELISA)], and molecular marker analyses. The expression of symptoms expression was evaluated on four occasions throughout the course of the experiment, while the presence of the virus in the plant was diagnosed by DAS-ELISA. In addition, the genotyping with simple sequence repeat (SSR) markers (PGS1.21, PGS1.23) and ParPMC2 allele-specific polymerase chain reaction (PCR) for the purpose of evaluating PPV resistance. A total of five new apricot hybrids were evaluated as resistant to PPV, while five hybrids exhibited resistance despite the presence of some PPV symptoms. In addition, three hybrids demonstrated genotype-phenotype incongruences (GPIs). The statistical analysis revealed a significant relation between PPV-positive plants and the absence of resistance-associated alleles, confirming that allelic composition is strongly associated with phenotypic resistance. The average value of symptoms evaluation was 0.3 ± 0.1 for resistant hybrids and 1.7 ± 0.3 and 1.5 ± 0.4 for susceptible hybrids evaluated by PGS1.21 and PGS1.23, respectively. The Tukey’s honestly significant difference (HSD) test showed the significant difference between genotypes evaluated by ParPMC2 marker as “RR” and “RS” (resistant) from “SS” (susceptible). However, discrepancies in hybrid resistance evaluation highlight the complexity of inheritance and the potential errors in genotyping. These results indicate that the most reliable method for evaluating PPV resistance is the time-consuming phenotyping method. Conversely, the use of SSR markers greatly improves the effectiveness of breedinghttps://journals.ashs.org/hortsci/view/journals/hortsci/60/2/article-p261.xmlplum pox virusprunus armeniacasharkastone fruit breeding |
| spellingShingle | Eliška Zezulová Tomáš Kiss Kateřina Baránková Tomáš Nečas Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids HortScience plum pox virus prunus armeniaca sharka stone fruit breeding |
| title | Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids |
| title_full | Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids |
| title_fullStr | Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids |
| title_full_unstemmed | Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids |
| title_short | Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids |
| title_sort | study of phenotypic and molecular approaches of potyvirus plumpoxi resistance evaluation in new czech apricot hybrids |
| topic | plum pox virus prunus armeniaca sharka stone fruit breeding |
| url | https://journals.ashs.org/hortsci/view/journals/hortsci/60/2/article-p261.xml |
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