Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂)
Triploid breeding is vital in aquaculture production. Here, based on the formation of all-female gynogenetic homologous diploid crucian carp (G, 2 n = 100) derived from gynogenesis of the autotetraploid crucian carp (4nRR, 4 n = 200) eggs and without chromosome duplication treatment, a novel triploi...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000067 |
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| author | Chongqing Wang Yan Tang Xiang Luo Yuxin Zhang Yue Zhou Qingwen Xiao Zhengkun Liu Xinyi Deng Xu Huang Xidan Xu Xiaowei Xu Kun Zhang Qinbo Qin Shaojun Liu |
| author_facet | Chongqing Wang Yan Tang Xiang Luo Yuxin Zhang Yue Zhou Qingwen Xiao Zhengkun Liu Xinyi Deng Xu Huang Xidan Xu Xiaowei Xu Kun Zhang Qinbo Qin Shaojun Liu |
| author_sort | Chongqing Wang |
| collection | DOAJ |
| description | Triploid breeding is vital in aquaculture production. Here, based on the formation of all-female gynogenetic homologous diploid crucian carp (G, 2 n = 100) derived from gynogenesis of the autotetraploid crucian carp (4nRR, 4 n = 200) eggs and without chromosome duplication treatment, a novel triploid fish (3nRR, 3 n = 150) were produced by crossing G and males of red crucian carp (RCC, 2 n = 100). All 3nRR possessed 150 chromosomes and were triploid hybrids. The genetic characteristics of 5S rDNA in 3nRR individuals and their parental counterparts, as well as the measurable and countable traits, were explored. The 3nRR males exhibited improper sperm development, whereas 3nRR females produced mature eggs by two years old. Females of 3nRR produced various-sized eggs that fertilized with haploid sperm from RCC, producing viable diploids, triploids and tetraploids. Analysis of gonadal transcriptome between 3nRR and RCC revealed that BMP2 was linked to fertility of female 3nRR, and SOX2 plays a significant role in the male sterility of 3nRR. The findings of this study suggested that the stable production of triploid fish could be achieved via the hybridization of diploid fish. The triploids have important application values in protecting germplasm resources and preparing polyploid fish. |
| format | Article |
| id | doaj-art-c1148f2d85954300aac2db0722199177 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-c1148f2d85954300aac2db07221991772025-02-06T05:12:20ZengElsevierAquaculture Reports2352-51342025-03-0140102620Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂)Chongqing Wang0Yan Tang1Xiang Luo2Yuxin Zhang3Yue Zhou4Qingwen Xiao5Zhengkun Liu6Xinyi Deng7Xu Huang8Xidan Xu9Xiaowei Xu10Kun Zhang11Qinbo Qin12Shaojun Liu13State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR China; Hunan Yuelu Mountain Scienece and Technology Co., Ltd. for Aquatic Breeding, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou, PR China; Corresponding author at: State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR China.State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR ChinaTriploid breeding is vital in aquaculture production. Here, based on the formation of all-female gynogenetic homologous diploid crucian carp (G, 2 n = 100) derived from gynogenesis of the autotetraploid crucian carp (4nRR, 4 n = 200) eggs and without chromosome duplication treatment, a novel triploid fish (3nRR, 3 n = 150) were produced by crossing G and males of red crucian carp (RCC, 2 n = 100). All 3nRR possessed 150 chromosomes and were triploid hybrids. The genetic characteristics of 5S rDNA in 3nRR individuals and their parental counterparts, as well as the measurable and countable traits, were explored. The 3nRR males exhibited improper sperm development, whereas 3nRR females produced mature eggs by two years old. Females of 3nRR produced various-sized eggs that fertilized with haploid sperm from RCC, producing viable diploids, triploids and tetraploids. Analysis of gonadal transcriptome between 3nRR and RCC revealed that BMP2 was linked to fertility of female 3nRR, and SOX2 plays a significant role in the male sterility of 3nRR. The findings of this study suggested that the stable production of triploid fish could be achieved via the hybridization of diploid fish. The triploids have important application values in protecting germplasm resources and preparing polyploid fish.http://www.sciencedirect.com/science/article/pii/S2352513425000067Novel triploid fishDiploid crossesAbnormal gonadal developmentTranscriptomic analysis |
| spellingShingle | Chongqing Wang Yan Tang Xiang Luo Yuxin Zhang Yue Zhou Qingwen Xiao Zhengkun Liu Xinyi Deng Xu Huang Xidan Xu Xiaowei Xu Kun Zhang Qinbo Qin Shaojun Liu Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) Aquaculture Reports Novel triploid fish Diploid crosses Abnormal gonadal development Transcriptomic analysis |
| title | Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) |
| title_full | Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) |
| title_fullStr | Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) |
| title_full_unstemmed | Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) |
| title_short | Production of all-triploid derived from gynogenetic homologous diploid crucian carp (♀) × diploid red crucian carp (♂) |
| title_sort | production of all triploid derived from gynogenetic homologous diploid crucian carp ♀ diploid red crucian carp ♂ |
| topic | Novel triploid fish Diploid crosses Abnormal gonadal development Transcriptomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000067 |
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