Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
Background. Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/1943980 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567057990811648 |
|---|---|
| author | Andrzej Eljaszewicz Lukasz Bolkun Kamil Grubczak Malgorzata Rusak Tomasz Wasiluk Milena Dabrowska Piotr Radziwon Wojciech Marlicz Karol Kamiński Janusz Kloczko Marcin Moniuszko |
| author_facet | Andrzej Eljaszewicz Lukasz Bolkun Kamil Grubczak Malgorzata Rusak Tomasz Wasiluk Milena Dabrowska Piotr Radziwon Wojciech Marlicz Karol Kamiński Janusz Kloczko Marcin Moniuszko |
| author_sort | Andrzej Eljaszewicz |
| collection | DOAJ |
| description | Background. Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated. Therefore, in this study we aimed to assess the effect of administration of G-CSF to ALL patients on mobilization of other than hematopoietic stem cell (HSCs) subsets, namely, very small embryonic-like stem cells (VSELs), endothelial progenitor cells (EPCs), and different monocyte subsets. Methods. We used multicolor flow cytometry to quantitate numbers of CD34+ cells, hematopoietic stem cells (HSCs), VSELs, EPCs, and different monocyte subsets in the peripheral blood of ALL patients and normal age-matched blood donors. Results. We showed that ALL patients following chemotherapy, when compared to healthy donors, presented with significantly lower numbers of CD34+ cells, HSCs, VSELs, and CD14+ monocytes, but not EPCs. Moreover, we found that G-CSF administration induced effective mobilization of all the abovementioned progenitor and stem cell subsets with high regenerative and proangiogenic potential. Conclusion. These findings contribute to better understanding the beneficial clinical effect of G-CSF administration in ALL patients following successful chemotherapy. |
| format | Article |
| id | doaj-art-c10f77794fe44076ba469d8da55ab81e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c10f77794fe44076ba469d8da55ab81e2025-02-03T01:02:28ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/19439801943980Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF TreatmentAndrzej Eljaszewicz0Lukasz Bolkun1Kamil Grubczak2Malgorzata Rusak3Tomasz Wasiluk4Milena Dabrowska5Piotr Radziwon6Wojciech Marlicz7Karol Kamiński8Janusz Kloczko9Marcin Moniuszko10Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Ul. Waszyngtona 13, 15-269 Bialystok, PolandDepartment of Haematology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Ul. Waszyngtona 13, 15-269 Bialystok, PolandDepartment of Hematological Diagnostics, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Haematology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Hematological Diagnostics, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Haematology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Gastroenterology, Pomeranian Medical University, Ul. Unii Lubelskiej 1, 71-252 Szczecin, PolandDepartment of Cardiology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Haematology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, PolandDepartment of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Ul. Waszyngtona 13, 15-269 Bialystok, PolandBackground. Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated. Therefore, in this study we aimed to assess the effect of administration of G-CSF to ALL patients on mobilization of other than hematopoietic stem cell (HSCs) subsets, namely, very small embryonic-like stem cells (VSELs), endothelial progenitor cells (EPCs), and different monocyte subsets. Methods. We used multicolor flow cytometry to quantitate numbers of CD34+ cells, hematopoietic stem cells (HSCs), VSELs, EPCs, and different monocyte subsets in the peripheral blood of ALL patients and normal age-matched blood donors. Results. We showed that ALL patients following chemotherapy, when compared to healthy donors, presented with significantly lower numbers of CD34+ cells, HSCs, VSELs, and CD14+ monocytes, but not EPCs. Moreover, we found that G-CSF administration induced effective mobilization of all the abovementioned progenitor and stem cell subsets with high regenerative and proangiogenic potential. Conclusion. These findings contribute to better understanding the beneficial clinical effect of G-CSF administration in ALL patients following successful chemotherapy.http://dx.doi.org/10.1155/2018/1943980 |
| spellingShingle | Andrzej Eljaszewicz Lukasz Bolkun Kamil Grubczak Malgorzata Rusak Tomasz Wasiluk Milena Dabrowska Piotr Radziwon Wojciech Marlicz Karol Kamiński Janusz Kloczko Marcin Moniuszko Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment Stem Cells International |
| title | Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment |
| title_full | Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment |
| title_fullStr | Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment |
| title_full_unstemmed | Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment |
| title_short | Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment |
| title_sort | very small embryonic like stem cells endothelial progenitor cells and different monocyte subsets are effectively mobilized in acute lymphoblastic leukemia patients after g csf treatment |
| url | http://dx.doi.org/10.1155/2018/1943980 |
| work_keys_str_mv | AT andrzejeljaszewicz verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT lukaszbolkun verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT kamilgrubczak verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT malgorzatarusak verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT tomaszwasiluk verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT milenadabrowska verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT piotrradziwon verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT wojciechmarlicz verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT karolkaminski verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT januszkloczko verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment AT marcinmoniuszko verysmallembryoniclikestemcellsendothelialprogenitorcellsanddifferentmonocytesubsetsareeffectivelymobilizedinacutelymphoblasticleukemiapatientsaftergcsftreatment |