OptiPrep™ Density Gradient Solutions for Mammalian Organelles
Any density gradient for the isolation of mammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic str...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.840 |
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| _version_ | 1832563316322467840 |
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| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | Any density gradient for the isolation of mammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA and DTT) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep™ in order to prepare such solutions for mammalian organelles and membranes. |
| format | Article |
| id | doaj-art-c10933136d2c48ea8cc37d6427fb4ef6 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c10933136d2c48ea8cc37d6427fb4ef62025-02-03T01:20:27ZengWileyThe Scientific World Journal1537-744X2002-01-0121440144310.1100/tsw.2002.840OptiPrep™ Density Gradient Solutions for Mammalian OrganellesJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKAny density gradient for the isolation of mammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA and DTT) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep™ in order to prepare such solutions for mammalian organelles and membranes.http://dx.doi.org/10.1100/tsw.2002.840 |
| spellingShingle | John Graham OptiPrep™ Density Gradient Solutions for Mammalian Organelles The Scientific World Journal |
| title | OptiPrep™ Density Gradient Solutions for Mammalian Organelles |
| title_full | OptiPrep™ Density Gradient Solutions for Mammalian Organelles |
| title_fullStr | OptiPrep™ Density Gradient Solutions for Mammalian Organelles |
| title_full_unstemmed | OptiPrep™ Density Gradient Solutions for Mammalian Organelles |
| title_short | OptiPrep™ Density Gradient Solutions for Mammalian Organelles |
| title_sort | optiprep™ density gradient solutions for mammalian organelles |
| url | http://dx.doi.org/10.1100/tsw.2002.840 |
| work_keys_str_mv | AT johngraham optiprepdensitygradientsolutionsformammalianorganelles |