IL-1 and its role in rat carrageenan pleurisy
The carrageenan pleurisy model, which is characterized by cellular influx and oedema, has been used to examine the effects of anti-inflammatory compounds such as naproxen. Interleukin-1α and β (IL-1) are known to be pro-inflammatory mediators, and their roles in this model are unknown. Intrapleural...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1993-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000043 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556294717833216 |
|---|---|
| author | R. Goodman L. R. Mantegna C. L. McAIlister E. Bruin R. L. Dowling H. George W. Feeser B. Freimark M. Lischwe S. Pick R. R. Harris J. S. Kerr |
| author_facet | R. Goodman L. R. Mantegna C. L. McAIlister E. Bruin R. L. Dowling H. George W. Feeser B. Freimark M. Lischwe S. Pick R. R. Harris J. S. Kerr |
| author_sort | R. Goodman |
| collection | DOAJ |
| description | The carrageenan pleurisy model, which is characterized by cellular influx and oedema, has been used to examine the effects of anti-inflammatory compounds such as naproxen. Interleukin-1α and β (IL-1) are known to be pro-inflammatory mediators, and their roles in this model are unknown. Intrapleural injection of 1% viscarin carrageenan or saline was administered to male Lewis rats. Four to 24 h later, cell counts, fluid volumes and IL-1β levels (measured by ELISA) were determined in the pleural cavity. Serum corticosterone levels were measured only at 4 h. Significant increases in IL-1β levels precede cell influx suggesting IL-1β plays a role in the maintenance of cell accumulation in the pleural cavity. None of the drugs tested, including the IL-1 receptor antagonist, maintained pleural cell influx and IL-1β levels at control levels. When human IL-1α or β or rat IL-1β were injected individually into the pleural cavity, none of these cytokines were pro-inflammatory, as measured by increased cell influx and fluid extravasation. These results suggest that although IL-1β levels increase in the pleural cavity in response to carrageenan, IL-1 per se is not the initiator of the pro-inflammatory events of cell influx and oedema in this model. |
| format | Article |
| id | doaj-art-c0fa3ac0cbd14908a8ab91e60fb102ca |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c0fa3ac0cbd14908a8ab91e60fb102ca2025-02-03T05:45:47ZengWileyMediators of Inflammation0962-93511466-18611993-01-0121333910.1155/S0962935193000043IL-1 and its role in rat carrageenan pleurisyR. Goodman0L. R. Mantegna1C. L. McAIlister2E. Bruin3R. L. Dowling4H. George5W. Feeser6B. Freimark7M. Lischwe8S. Pick9R. R. Harris10J. S. Kerr11The DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe DuPont Merck Pharmaceutical Company, Inflammatory Diseases Research, Experimental Station E400/4223, Wilmington, DE 19898-0400, USAThe carrageenan pleurisy model, which is characterized by cellular influx and oedema, has been used to examine the effects of anti-inflammatory compounds such as naproxen. Interleukin-1α and β (IL-1) are known to be pro-inflammatory mediators, and their roles in this model are unknown. Intrapleural injection of 1% viscarin carrageenan or saline was administered to male Lewis rats. Four to 24 h later, cell counts, fluid volumes and IL-1β levels (measured by ELISA) were determined in the pleural cavity. Serum corticosterone levels were measured only at 4 h. Significant increases in IL-1β levels precede cell influx suggesting IL-1β plays a role in the maintenance of cell accumulation in the pleural cavity. None of the drugs tested, including the IL-1 receptor antagonist, maintained pleural cell influx and IL-1β levels at control levels. When human IL-1α or β or rat IL-1β were injected individually into the pleural cavity, none of these cytokines were pro-inflammatory, as measured by increased cell influx and fluid extravasation. These results suggest that although IL-1β levels increase in the pleural cavity in response to carrageenan, IL-1 per se is not the initiator of the pro-inflammatory events of cell influx and oedema in this model.http://dx.doi.org/10.1155/S0962935193000043 |
| spellingShingle | R. Goodman L. R. Mantegna C. L. McAIlister E. Bruin R. L. Dowling H. George W. Feeser B. Freimark M. Lischwe S. Pick R. R. Harris J. S. Kerr IL-1 and its role in rat carrageenan pleurisy Mediators of Inflammation |
| title | IL-1 and its role in rat carrageenan pleurisy |
| title_full | IL-1 and its role in rat carrageenan pleurisy |
| title_fullStr | IL-1 and its role in rat carrageenan pleurisy |
| title_full_unstemmed | IL-1 and its role in rat carrageenan pleurisy |
| title_short | IL-1 and its role in rat carrageenan pleurisy |
| title_sort | il 1 and its role in rat carrageenan pleurisy |
| url | http://dx.doi.org/10.1155/S0962935193000043 |
| work_keys_str_mv | AT rgoodman il1anditsroleinratcarrageenanpleurisy AT lrmantegna il1anditsroleinratcarrageenanpleurisy AT clmcailister il1anditsroleinratcarrageenanpleurisy AT ebruin il1anditsroleinratcarrageenanpleurisy AT rldowling il1anditsroleinratcarrageenanpleurisy AT hgeorge il1anditsroleinratcarrageenanpleurisy AT wfeeser il1anditsroleinratcarrageenanpleurisy AT bfreimark il1anditsroleinratcarrageenanpleurisy AT mlischwe il1anditsroleinratcarrageenanpleurisy AT spick il1anditsroleinratcarrageenanpleurisy AT rrharris il1anditsroleinratcarrageenanpleurisy AT jskerr il1anditsroleinratcarrageenanpleurisy |