Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation
BackgroundPeritoneal macrophages, comprising large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), play a vital role in maintaining immune defenses during inflammation. However, the molecular mechanisms governing their responses, particularly the impact of retinoic acid (RA),...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1545720/full |
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| author | Yujuan Qin Yujuan Qin Xi Wang Xi Wang Xiamin Zhang Xiamin Zhang Lianting Nong Lianting Nong Qiyan Hou Yuhong Chen Yuhong Chen Yuting Li Yuting Li Wenxian Lin Xiuli Mao Kezhao Wu Wenqian Nong Tonghua Wang Lingzhang Meng Lingzhang Meng Jian Song Jian Song Jian Song |
| author_facet | Yujuan Qin Yujuan Qin Xi Wang Xi Wang Xiamin Zhang Xiamin Zhang Lianting Nong Lianting Nong Qiyan Hou Yuhong Chen Yuhong Chen Yuting Li Yuting Li Wenxian Lin Xiuli Mao Kezhao Wu Wenqian Nong Tonghua Wang Lingzhang Meng Lingzhang Meng Jian Song Jian Song Jian Song |
| author_sort | Yujuan Qin |
| collection | DOAJ |
| description | BackgroundPeritoneal macrophages, comprising large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), play a vital role in maintaining immune defenses during inflammation. However, the molecular mechanisms governing their responses, particularly the impact of retinoic acid (RA), remain poorly understood. This study aims to elucidate the role of RA in modulating macrophage function, distribution, and immune responses during bacterial infections.MethodsA murine model of peritonitis was established using Escherichia coli expressing a tdTomato fluorescence marker. The effects of RA on macrophage phagocytic capacity, population dynamics, and transcriptomic profiles were assessed using immunofluorescence, flow cytometry, RNA sequencing, and quantitative PCR. Additionally, RA-loaded ZIF-8 nanoparticles were employed to investigate the sustained effects of RA delivery.ResultsRA significantly enhanced macrophage phagocytic activity, delayed functional decline, and promoted the recruitment of SPMs in the peritoneal cavity. Transcriptomic analysis revealed upregulation of leukocyte migration and cell adhesion pathways in RA-treated SPMs. RA treatment also induced distinct gene expression profiles in macrophage subpopulations, reflecting its role in immune modulation. Notably, RA-loaded ZIF-8 nanoparticles prolonged RA retention within macrophages, sustaining its effects.ConclusionRA enhances antibacterial defense by modulating macrophage activity, providing new insights into immune regulation. These findings underscore the therapeutic potential of RA and its nanoparticle formulations in managing bacterial infections and inflammation. |
| format | Article |
| id | doaj-art-c0f755be3c6044c2bc172dd5e2a8e202 |
| institution | OA Journals |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-c0f755be3c6044c2bc172dd5e2a8e2022025-08-20T01:48:20ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-04-011210.3389/fnut.2025.15457201545720Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammationYujuan Qin0Yujuan Qin1Xi Wang2Xi Wang3Xiamin Zhang4Xiamin Zhang5Lianting Nong6Lianting Nong7Qiyan Hou8Yuhong Chen9Yuhong Chen10Yuting Li11Yuting Li12Wenxian Lin13Xiuli Mao14Kezhao Wu15Wenqian Nong16Tonghua Wang17Lingzhang Meng18Lingzhang Meng19Jian Song20Jian Song21Jian Song22Graduate School, Youjiang Medical University for Nationalities, Baise, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaSchool of Medicine, Guangxi University, Nanning, ChinaGraduate School, Youjiang Medical University for Nationalities, Baise, ChinaDepartment of Digestive Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaGraduate School, Youjiang Medical University for Nationalities, Baise, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaGraduate School, Youjiang Medical University for Nationalities, Baise, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaGraduate School, Youjiang Medical University for Nationalities, Baise, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Digestive Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaGraduate School, Youjiang Medical University for Nationalities, Baise, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaGraduate School, Youjiang Medical University for Nationalities, Baise, ChinaInstitute of Cardiovascular Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, ChinaInstitute of Physiological Chemistry and Pathobiochemistry, University of Münster, Münster, GermanyBackgroundPeritoneal macrophages, comprising large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), play a vital role in maintaining immune defenses during inflammation. However, the molecular mechanisms governing their responses, particularly the impact of retinoic acid (RA), remain poorly understood. This study aims to elucidate the role of RA in modulating macrophage function, distribution, and immune responses during bacterial infections.MethodsA murine model of peritonitis was established using Escherichia coli expressing a tdTomato fluorescence marker. The effects of RA on macrophage phagocytic capacity, population dynamics, and transcriptomic profiles were assessed using immunofluorescence, flow cytometry, RNA sequencing, and quantitative PCR. Additionally, RA-loaded ZIF-8 nanoparticles were employed to investigate the sustained effects of RA delivery.ResultsRA significantly enhanced macrophage phagocytic activity, delayed functional decline, and promoted the recruitment of SPMs in the peritoneal cavity. Transcriptomic analysis revealed upregulation of leukocyte migration and cell adhesion pathways in RA-treated SPMs. RA treatment also induced distinct gene expression profiles in macrophage subpopulations, reflecting its role in immune modulation. Notably, RA-loaded ZIF-8 nanoparticles prolonged RA retention within macrophages, sustaining its effects.ConclusionRA enhances antibacterial defense by modulating macrophage activity, providing new insights into immune regulation. These findings underscore the therapeutic potential of RA and its nanoparticle formulations in managing bacterial infections and inflammation.https://www.frontiersin.org/articles/10.3389/fnut.2025.1545720/fullperitonitislarge peritoneal macrophagessmall peritoneal macrophagesretinoic acidnanoparticles |
| spellingShingle | Yujuan Qin Yujuan Qin Xi Wang Xi Wang Xiamin Zhang Xiamin Zhang Lianting Nong Lianting Nong Qiyan Hou Yuhong Chen Yuhong Chen Yuting Li Yuting Li Wenxian Lin Xiuli Mao Kezhao Wu Wenqian Nong Tonghua Wang Lingzhang Meng Lingzhang Meng Jian Song Jian Song Jian Song Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation Frontiers in Nutrition peritonitis large peritoneal macrophages small peritoneal macrophages retinoic acid nanoparticles |
| title | Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation |
| title_full | Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation |
| title_fullStr | Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation |
| title_full_unstemmed | Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation |
| title_short | Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation |
| title_sort | retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation |
| topic | peritonitis large peritoneal macrophages small peritoneal macrophages retinoic acid nanoparticles |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1545720/full |
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